Sample Set Annotation Tool: Effects of exercise on gene expression level in human monocytes-GSE51835

Study Design
Assay
Sample Info
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Samples
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Analysis

Purpose

We tested the hypothesis on the mechanisms responsible for the early control of monocytes function by identifying a discrete set of genes in circulating monocytes that were altered by exercise.Exercise leads to a rapid change in t

Hypothesis

Exercise leads to a rapid change in the profile of gene expression in monocytes.

Experimental Design

Twelve healthy men (22-30 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 82% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. Monocytes were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Monocyte Isolation Kit II human #130-091-153 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®. For this study we used Affymetrix plus 2 (total of 24 chips).

Experimental Variables

Controls

Methods

Additional Information

Radom-Aizik S, Zaldivar FP Jr, Haddad F, Cooper DM. Impact of brief exercise on circulating monocyte gene and microRNA expression: implications for atherosclerotic vascular disease. Brain Behav Immun 2014 Jul;39:121-9. PMID: 24423463

Expected Samples:

24 Samples Loaded: 24

Species

Human (Homo sapiens)

Sample Source

Monocytes

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Sample ID	Age	Gender	!Sample_title	time point	tissue
GSM1253472	22-30 yr old	male	Mono_before exercise_01	before exercise	monocytes isolated from PBMC
GSM1253473	22-30 yr old	male	Mono_after exercise_01	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253474	22-30 yr old	male	Mono_before exercise_03	before exercise	monocytes isolated from PBMC
GSM1253475	22-30 yr old	male	Mono_after exercise_03	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253476	22-30 yr old	male	Mono_before exercise_04	before exercise	monocytes isolated from PBMC

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Sample ID	Age	Gender	!Sample Title	Time point	Tissue
GSM1253472	22-30 yr old	male	Mono_before exercise_01	before exercise	monocytes isolated from PBMC
GSM1253473	22-30 yr old	male	Mono_after exercise_01	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253474	22-30 yr old	male	Mono_before exercise_03	before exercise	monocytes isolated from PBMC
GSM1253475	22-30 yr old	male	Mono_after exercise_03	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253476	22-30 yr old	male	Mono_before exercise_04	before exercise	monocytes isolated from PBMC
GSM1253477	22-30 yr old	male	Mono_after exercise_04	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253478	22-30 yr old	male	Mono_before exercise_05	before exercise	monocytes isolated from PBMC
GSM1253479	22-30 yr old	male	Mono_after exercise_05	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253480	22-30 yr old	male	Mono_before exercise_06	before exercise	monocytes isolated from PBMC
GSM1253481	22-30 yr old	male	Mono_after exercise_06	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253482	22-30 yr old	male	Mono_before exercise_07	before exercise	monocytes isolated from PBMC
GSM1253483	22-30 yr old	male	Mono_after exercise_07	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253484	22-30 yr old	male	Mono_before exercise_08	before exercise	monocytes isolated from PBMC
GSM1253485	22-30 yr old	male	Mono_after exercise_08	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253486	22-30 yr old	male	Mono_before exercise_09	before exercise	monocytes isolated from PBMC
GSM1253487	22-30 yr old	male	Mono_after exercise_09	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253488	22-30 yr old	male	Mono_before exercise_11	before exercise	monocytes isolated from PBMC
GSM1253489	22-30 yr old	male	Mono_after exercise_11	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253490	22-30 yr old	male	Mono_before exercise_12	before exercise	monocytes isolated from PBMC
GSM1253491	22-30 yr old	male	Mono_after exercise_12	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253492	22-30 yr old	male	Mono_before exercise_13	before exercise	monocytes isolated from PBMC
GSM1253493	22-30 yr old	male	Mono_after exercise_13	after 20 min bout of exercise	monocytes isolated from PBMC
GSM1253494	22-30 yr old	male	Mono_before exercise_14	before exercise	monocytes isolated from PBMC
GSM1253495	22-30 yr old	male	Mono_after exercise_14	after 20 min bout of exercise	monocytes isolated from PBMC

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