Description | Effects of exercise on gene expression level in human monocytes-GSE51835 |
Purpose | We tested the hypothesis on the mechanisms responsible for the early control of monocytes function by identifying a discrete set of genes in circulating monocytes that were altered by exercise.Exercise leads to a rapid change in t |
Hypothesis | Exercise leads to a rapid change in the profile of gene expression in monocytes. |
Experimental Design | Twelve healthy men (22-30 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 82% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. Monocytes were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Monocyte Isolation Kit II human #130-091-153 and the autoMACSĀ® Pro Separator). Total RNA was extracted using TRIzolĀ®. For this study we used Affymetrix plus 2 (total of 24 chips). |
Additional Information | |
Platform | Affymetrix HG-U133_Plus_2 |
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