Effects of exercise on gene expression level in human monocytes-GSE51835
We tested the hypothesis on the mechanisms responsible for the early control of monocytes function by identifying a discrete set of genes in circulating monocytes that were altered by exercise.Exercise leads to a rapid change in t
Exercise leads to a rapid change in the profile of gene expression in monocytes.
Twelve healthy men (22-30 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 82% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. Monocytes were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Monocyte Isolation Kit II human #130-091-153 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®. For this study we used Affymetrix plus 2 (total of 24 chips).
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Sample ID | Age | Gender | !Sample_title | time point | tissue |
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GSM1253472 | 22-30 yr old | male | Mono_before exercise_01 | before exercise | monocytes isolated from PBMC |
GSM1253473 | 22-30 yr old | male | Mono_after exercise_01 | after 20 min bout of exercise | monocytes isolated from PBMC |
GSM1253474 | 22-30 yr old | male | Mono_before exercise_03 | before exercise | monocytes isolated from PBMC |
GSM1253475 | 22-30 yr old | male | Mono_after exercise_03 | after 20 min bout of exercise | monocytes isolated from PBMC |
GSM1253476 | 22-30 yr old | male | Mono_before exercise_04 | before exercise | monocytes isolated from PBMC |
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Sample ID | Age | Gender | !Sample Title | Time point | Tissue |
---|---|---|---|---|---|
GSM1253472 | 22-30 yr old |
male |
Mono_before exercise_01 |
before exercise |
monocytes isolated from PBMC |
GSM1253473 | 22-30 yr old |
male |
Mono_after exercise_01 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253474 | 22-30 yr old |
male |
Mono_before exercise_03 |
before exercise |
monocytes isolated from PBMC |
GSM1253475 | 22-30 yr old |
male |
Mono_after exercise_03 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253476 | 22-30 yr old |
male |
Mono_before exercise_04 |
before exercise |
monocytes isolated from PBMC |
GSM1253477 | 22-30 yr old |
male |
Mono_after exercise_04 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253478 | 22-30 yr old |
male |
Mono_before exercise_05 |
before exercise |
monocytes isolated from PBMC |
GSM1253479 | 22-30 yr old |
male |
Mono_after exercise_05 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253480 | 22-30 yr old |
male |
Mono_before exercise_06 |
before exercise |
monocytes isolated from PBMC |
GSM1253481 | 22-30 yr old |
male |
Mono_after exercise_06 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253482 | 22-30 yr old |
male |
Mono_before exercise_07 |
before exercise |
monocytes isolated from PBMC |
GSM1253483 | 22-30 yr old |
male |
Mono_after exercise_07 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253484 | 22-30 yr old |
male |
Mono_before exercise_08 |
before exercise |
monocytes isolated from PBMC |
GSM1253485 | 22-30 yr old |
male |
Mono_after exercise_08 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253486 | 22-30 yr old |
male |
Mono_before exercise_09 |
before exercise |
monocytes isolated from PBMC |
GSM1253487 | 22-30 yr old |
male |
Mono_after exercise_09 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253488 | 22-30 yr old |
male |
Mono_before exercise_11 |
before exercise |
monocytes isolated from PBMC |
GSM1253489 | 22-30 yr old |
male |
Mono_after exercise_11 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253490 | 22-30 yr old |
male |
Mono_before exercise_12 |
before exercise |
monocytes isolated from PBMC |
GSM1253491 | 22-30 yr old |
male |
Mono_after exercise_12 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253492 | 22-30 yr old |
male |
Mono_before exercise_13 |
before exercise |
monocytes isolated from PBMC |
GSM1253493 | 22-30 yr old |
male |
Mono_after exercise_13 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
GSM1253494 | 22-30 yr old |
male |
Mono_before exercise_14 |
before exercise |
monocytes isolated from PBMC |
GSM1253495 | 22-30 yr old |
male |
Mono_after exercise_14 |
after 20 min bout of exercise |
monocytes isolated from PBMC |
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