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Clinical

Genome-wide analysis of monocytes and T cells' response to interferon beta-GSE34627

Purpose

To characterized the IFN-? transcriptional response in monocytes, a small but influential cell-subset, using gene expression profile and pathway analysis.

Hypothesis

The unique expression profile of monocytes in response to Interferon-beta (IFN-?) might be masked by the response profile of the more abundant T cells.

Experimental Design

Monocytes and T lymphocytes were isolated from 3 healthy volunteers. For each sample the expression profile of untreated cells was compared to the profile of IFN-? treated cells, i.e. 4 samples from each donor (2 cell types, with and without treatment), 12 samples total.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Henig N, Avidan N, Mandel I, Staun-Ram E et al. Interferon-beta induces distinct gene expression response patterns in human monocytes versus T cells.PLoS One 2013;8(4):e62366. PMID: 23626809
  • Microarray
    Illumina HumanWG-6 v3
    12 Samples Loaded: 12
    Human (Homo sapiens)
    T cells , Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title donor cell type Culture conditions treated
    GSM852149 T lymphocytes not treated with IFN-β, individual 1 Individual1 T lymphocytes T cell_control No
    GSM852150 T lymphocytes treated with IFN-β, individual 1 Individual1 T lymphocytes T cell+IFNb Yes
    GSM852151 Monocytes not treated with IFN-β, individual 1 Individual1 Monocytes Mono_control No
    GSM852152 Monocytes treated with IFN-β, individual 1 Individual1 Monocytes Mono+IFNb Yes
    GSM852153 T lymphocytes not treated with IFN-β, individual 2 Individual2 T lymphocytes T cell_control No
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    File
    sampleset4000093_sampleannotations.csv
    Sample Set Spreadsheet
    Log2 Transformed
    Raw Signal
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    Sample ID !Sample Title Donor Cell type Culture conditions Treated
    GSM852149
    T lymphocytes not treated with IFN-β, individual 1
    Individual1
    T lymphocytes
    T cell_control
    No
    GSM852150
    T lymphocytes treated with IFN-β, individual 1
    Individual1
    T lymphocytes
    T cell+IFNb
    Yes
    GSM852151
    Monocytes not treated with IFN-β, individual 1
    Individual1
    Monocytes
    Mono_control
    No
    GSM852152
    Monocytes treated with IFN-β, individual 1
    Individual1
    Monocytes
    Mono+IFNb
    Yes
    GSM852153
    T lymphocytes not treated with IFN-β, individual 2
    Individual2
    T lymphocytes
    T cell_control
    No
    GSM852154
    T lymphocytes treated with IFN-β, individual 2
    Individual2
    T lymphocytes
    T cell+IFNb
    Yes
    GSM852155
    Monocytes not treated with IFN-β, individual 2
    Individual2
    Monocytes
    Mono_control
    No
    GSM852156
    Monocytes treated with IFN-β, individual 2
    Individual2
    Monocytes
    Mono+IFNb
    Yes
    GSM852157
    T lymphocytes not treated with IFN-β, individual 3
    Individual3
    T lymphocytes
    T cell_control
    No
    GSM852158
    T lymphocytes treated with IFN-β, individual 3
    Individual3
    T lymphocytes
    T cell+IFNb
    Yes
    GSM852159
    Monocytes not treated with IFN-β, individual 3
    Individual3
    Monocytes
    Mono_control
    No
    GSM852160
    Monocytes treated with IFN-β, individual 3
    Individual3
    Monocytes
    Mono+IFNb
    Yes

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