Sample Set Annotation Tool: Leukotriene D4 induces gene expression in human monocytes through cysteinyl leukotriene type I receptor-GSE7807

Study Design
Assay
Sample Info
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Info
Samples
Group Sets
Analysis

Purpose

To determine the gene expression signature of human monocytes in response to cysLTs and to elucidate the signaling pathways involved in monocyte activation.

Hypothesis

Experimental Design

4 control sample, 4 LTD4 stimulated samples

Experimental Variables

Controls

Methods

Gene expression was analyzed using oligonucleotide microarrays. Responsiveness to cysLTs was assessed by real-time PCR, calcium flux, kinase activation and chemotaxis assays.

Additional Information

Woszczek G, Chen LY, Nagineni S, Kern S et al. Leukotriene D(4) induces gene expression in human monocytes through cysteinyl leukotriene type I receptor.J Allergy Clin Immunol 2008 Jan;121(1):215-221.e1. PMID: 18028998

Expected Samples:

8 Samples Loaded: 8

Species

Human (Homo sapiens)

Sample Source

Monocytes

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Sample ID	!Sample_title	Culture conditions	Monocytes exposed to vehicle control (EtOH)	Treatments
GSM189447	Donor 1 - control	Monocyte	True	Monocytes exposed to vehicle control(EtOH)
GSM189448	Donor 2 - control	Monocyte	True	Monocytes exposed to vehicle control(EtOH)
GSM189449	Donor 3 - control	Monocyte	True	Monocytes exposed to vehicle control(EtOH)
GSM189450	Donor 4 - control	Monocyte	True	Monocytes exposed to vehicle control(EtOH)
GSM189451	Donor 1 - LTD4 stimulated	Monocyte+LTD4		Monocytes stimulated with LTD4 (100nm) for 30 min

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File
sampleset4000083_sampleannotations.csv Sample Set Spreadsheet

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Sample ID	!Sample Title	Culture conditions	Monocytes exposed to vehicle control (EtOH)	Treatments
GSM189447	Donor 1 - control	Monocyte	True	Monocytes exposed to vehicle control(EtOH)
GSM189448	Donor 2 - control	Monocyte	True	Monocytes exposed to vehicle control(EtOH)
GSM189449	Donor 3 - control	Monocyte	True	Monocytes exposed to vehicle control(EtOH)
GSM189450	Donor 4 - control	Monocyte	True	Monocytes exposed to vehicle control(EtOH)
GSM189451	Donor 1 - LTD4 stimulated	Monocyte+LTD4		Monocytes stimulated with LTD4 (100nm) for 30 min
GSM189452	Donor 2 - LTD4 stimulated	Monocyte+LTD4		Monocytes stimulated with LTD4 (100nm) for 30 min
GSM189453	Donor 3 - LTD4 stimulated	Monocyte+LTD4		Monocytes stimulated with LTD4 (100nm) for 30 min
GSM189454	Donor 4 - LTD4 stimulated	Monocyte+LTD4		Monocytes stimulated with LTD4 (100nm) for 30 min

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