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Restricted Dendritic Cell and Monocyte Progenitors in Human Cord Blood and Bone Marrow-GSE65128

In Progress
Clinical

Restricted Dendritic Cell and Monocyte Progenitors in Human Cord Blood and Bone Marrow-GSE65128

Purpose

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Hypothesis

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Experimental Design

We performed whole transcriptome expression analysis on monocytes and subsets of dendritic cells i.e. CD1c+ DCs, CD141+ DCs and CD303+ pDCs isolated from blood or differentiated in culture from cord blood CD34+ cells in presence of MS5 stromal cells and Flt3l, GM-CSF and SCF cytokines.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Lee J, Breton G, Oliveira TY, Zhou YJ et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med 2015 Mar 9;212(3):385-99. PMID: 25687283
    • Microarray
    Illumina HumanHT-12 v4
    36 Samples Loaded: 36
    Human (Homo sapiens)
    pDC , Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title donor treatment sampleID cell subset
    GSM1587749 CD1c+ cDCs_blood_2 [8363877084_D] 2 none S20 CD1c+ cDC
    GSM1587750 CD1c+ cDCs_blood_4 [8363877084_E] 4 none S22 CD1c+ cDC
    GSM1587751 pDCs_blood_4 [8363877100_B] 4 none S10 pDC
    GSM1587752 CD141+ cDCs_blood_2 [8363877100_I] 2 none S14 CD141+ cDC
    GSM1587753 CD141+ cDCs_blood_5 [8363877100_K] 5 none S17 CD141+ cDC

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    Browse Files

    File
    sampleset4000058_sampleannotations.csv
    Sample Set Spreadsheet
    Log2 Transformed
    Raw Signal
    •   |  View in GXB »
    Clinical Datasource
    Links
    http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE65128
    http://www.ncbi.nlm.nih.gov/pubmed/25687283

    Samples Viewer / Editor

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    Sample ID !Sample Title Donor Treatment SampleID Cell subset Treatment conditions
    GSM1587749
    CD1c+ cDCs_blood_2 [8363877084_D]
    2
    none
    S20
    CD1c+ cDC
    CD1c+ cDC
    GSM1587750
    CD1c+ cDCs_blood_4 [8363877084_E]
    4
    none
    S22
    CD1c+ cDC
    CD1c+ cDC
    GSM1587751
    pDCs_blood_4 [8363877100_B]
    4
    none
    S10
    pDC
    pDC
    GSM1587752
    CD141+ cDCs_blood_2 [8363877100_I]
    2
    none
    S14
    CD141+ cDC
    CD141+ cDC
    GSM1587753
    CD141+ cDCs_blood_5 [8363877100_K]
    5
    none
    S17
    CD141+ cDC
    CD141+ cDC
    GSM1587754
    Monocytes_blood_6 [8363885001_K]
    6
    none
    S6
    monocytes
    monocytes
    GSM1587755
    CD1c+ cDCs_blood_3 [8363885004_F]
    3
    none
    S21
    CD1c+ cDC
    CD1c+ cDC
    GSM1587756
    pDCs_blood_1 [8363885008_A]
    1
    none
    S7
    pDC
    pDC
    GSM1587757
    CD1c+ cDCs_blood_6 [8363885008_C]
    6
    none
    S24
    CD1c+ cDC
    CD1c+ cDC
    GSM1587758
    CD141+ cDCs_blood_3 [8363885008_G]
    3
    none
    S15
    CD141+ cDC
    CD141+ cDC
    GSM1587759
    Monocytes_blood_1 [8363885011_E]
    1
    none
    S1
    monocytes
    monocytes
    GSM1587760
    CD141+ cDCs_blood_4 [8363885011_J ]
    4
    none
    S16
    CD141+ cDC
    CD141+ cDC
    GSM1587761
    pDCs_blood_6 [8363885011_L]
    6
    none
    S12
    pDC
    pDC
    GSM1587762
    CD141+ cDCs_blood_1 [8363885012_E]
    1
    none
    S13
    CD141+ cDC
    CD141+ cDC
    GSM1587763
    Monocytes_blood_2 [8363885012_H]
    2
    none
    S2
    monocytes
    monocytes
    GSM1587764
    Monocytes_blood_5 [8363885016_A]
    5
    none
    S5
    monocytes
    monocytes
    GSM1587765
    pDCs_blood_2 [8363885018_C]
    2
    none
    S8
    pDC
    pDC
    GSM1587766
    pDCs_blood_5 [8363885021_A]
    5
    none
    S11
    pDC
    pDC
    GSM1587767
    CD1c+ cDCs_blood_1 [8363885022_F]
    1
    none
    S19
    CD1c+ cDC
    CD1c+ cDC
    GSM1587768
    pDCs_blood_3 [8363885022_H]
    3
    none
    S9
    pDC
    pDC
    GSM1587769
    CD141+ cDCs_blood_6 [8363885026_A]
    6
    none
    S18
    CD141+ cDC
    CD141+ cDC
    GSM1587770
    CD1c+ cDCs_culture_8 [9406915091_C]
    8
    MS5 + Flt3l + SCF + GM-CSF
    S38
    CD1c+ cDC
    CD1c+ cDC_Treat
    GSM1587771
    CD1c+ cDCs_culture_10 [9406915091_D]
    10
    MS5 + Flt3l + SCF + GM-CSF
    S40
    CD1c+ cDC
    CD1c+ cDC_Treat
    GSM1587772
    Monocytes_culture_7 [9406915091_E]
    7
    MS5 + Flt3l + SCF + GM-CSF
    S25
    monocytes
    monocytes_Treat
    GSM1587773
    Monocytes_culture_8 [9406915091_G]
    8
    MS5 + Flt3l + SCF + GM-CSF
    S26
    monocytes
    monocytes_Treat
    GSM1587774
    pDCs_culture_9 [9406915091_H]
    9
    MS5 + Flt3l + SCF + GM-CSF
    S31
    pDC
    pDC_Treat
    GSM1587775
    CD141+ cDCs_culture_9 [9406915091_K]
    9
    MS5 + Flt3l + SCF + GM-CSF
    S35
    CD141+ cDC
    CD141+ cDC_Treat
    GSM1587776
    CD1c+ cDCs_culture_9 [9406915091_L]
    9
    MS5 + Flt3l + SCF + GM-CSF
    S39
    CD1c+ cDC
    CD1c+ cDC_Treat
    GSM1587777
    Monocytes_culture_9 [9407205014_B]
    9
    MS5 + Flt3l + SCF + GM-CSF
    S27
    monocytes
    monocytes_Treat
    GSM1587778
    pDCs_culture_8 [9407205014_C]
    8
    MS5 + Flt3l + SCF + GM-CSF
    S30
    pDC
    pDC_Treat
    GSM1587779
    CD1c+ cDCs_culture_7 [9407205014_E]
    7
    MS5 + Flt3l + SCF + GM-CSF
    S37
    CD1c+ cDC
    CD1c+ cDC_Treat
    GSM1587780
    pDCs_culture_7 [9407205014_F]
    7
    MS5 + Flt3l + SCF + GM-CSF
    S29
    pDC
    pDC_Treat
    GSM1587781
    pDCs_culture_10 [9407205014_G]
    10
    MS5 + Flt3l + SCF + GM-CSF
    S32
    pDC
    pDC_Treat
    GSM1587782
    CD141+ cDCs_culture_7 [9407205014_H]
    7
    MS5 + Flt3l + SCF + GM-CSF
    S33
    CD141+ cDC
    CD141+ cDC_Treat
    GSM1587783
    CD141+ cDCs_culture_8 [9407205014_I]
    8
    MS5 + Flt3l + SCF + GM-CSF
    S34
    CD141+ cDC
    CD141+ cDC_Treat
    GSM1587784
    CD141+ cDCs_culture_10 [9407205014_J]
    10
    MS5 + Flt3l + SCF + GM-CSF
    S36
    CD141+ cDC
    CD141+ cDC_Treat

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