Similarities and differences between human activated macrophages -GSE49240
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The human AA dataset 5 includes the gene expression profile of monocyte-derived macrophages isolated from blood donor buffy coats and plated on TCP in medium containing 10% autologous serum. Cells were stimulated for 120 hours with 50 ng/ml of IL-4, 50 U/ml of IFN-? plus 12.5 ng/ml of TNF-?, 50 ng/ml of M-CSF or 50 ng/ml of IL-10. RNA samples were isolated using TriPure isolation reagent (Roche) and the RNeasy Mini Kit-Qiagen Clean up method. The transcriptional profile was evaluated in two independent cell preparations, each derived from a different single donor using the HT12 V4 R2 bead chip arrays from Illumina.
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Total RNA obtained from monocyte-derived macrophages exposed to representative polarizing cytokines
| Sample ID | !Sample_title | cell type | culture condition |
|---|---|---|---|
| GSM1195728 | Monocytes (SAMPLE 1) | monocytes | Monocytes directly after isolation |
| GSM1195729 | Monocytes (SAMPLE 2) | monocytes | Monocytes directly after isolation |
| GSM1195730 | Medium (SAMPLE 3) | monocyte-derived macrophages | 10% Human serum |
| GSM1195731 | Medium (SAMPLE 4) | monocyte-derived macrophages | 10% Human serum |
| GSM1195732 | M-CSF (SAMPLE 5) | monocyte-derived macrophages | 10% Human serum+50 ng/ml of M-CSF |
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| Sample ID | !Sample Title | Cell type | Culture condition |
|---|---|---|---|
| GSM1195728 | Monocytes (SAMPLE 1) |
monocytes |
Monocytes directly after isolation |
| GSM1195729 | Monocytes (SAMPLE 2) |
monocytes |
Monocytes directly after isolation |
| GSM1195730 | Medium (SAMPLE 3) |
monocyte-derived macrophages |
10% Human serum |
| GSM1195731 | Medium (SAMPLE 4) |
monocyte-derived macrophages |
10% Human serum |
| GSM1195732 | M-CSF (SAMPLE 5) |
monocyte-derived macrophages |
10% Human serum+50 ng/ml of M-CSF |
| GSM1195733 | M-CSF (SAMPLE 6) |
monocyte-derived macrophages |
10% Human serum+50 ng/ml of M-CSF |
| GSM1195734 | IL-10 (SAMPLE 7) |
monocyte-derived macrophages |
10% Human serum+50 ng/ml of IL-4 |
| GSM1195735 | IL-10 (SAMPLE 8) |
monocyte-derived macrophages |
10% Human serum+50 ng/ml of IL-4 |
| GSM1195736 | IL-4 (SAMPLE 9) |
monocyte-derived macrophages |
10% Human serum+50 ng/ml of IL-10 |
| GSM1195737 | IL-4 (SAMPLE 10) |
monocyte-derived macrophages |
10% Human serum+50 ng/ml of IL-10 |
| GSM1195738 | IFN-y (SAMPLE 11) |
monocyte-derived macrophages |
10% Human serum+50 U/ml of IFN-γ plus 12.5 ng/ml of TNF-α |
| GSM1195739 | IFN-y (SAMPLE 12) |
monocyte-derived macrophages |
10% Human serum+50 U/ml of IFN-γ plus 12.5 ng/ml of TNF-α |
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