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SIDRA

GXB

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Description

Similarities and differences between human activated macrophages -GSE49240

Experimental Design

The human AA dataset 5 includes the gene expression profile of monocyte-derived macrophages isolated from blood donor buffy coats and plated on TCP in medium containing 10% autologous serum. Cells were stimulated for 120 hours with 50 ng/ml of IL-4, 50 U/ml of IFN-? plus 12.5 ng/ml of TNF-?, 50 ng/ml of M-CSF or 50 ng/ml of IL-10. RNA samples were isolated using TriPure isolation reagent (Roche) and the RNeasy Mini Kit-Qiagen Clean up method. The transcriptional profile was evaluated in two independent cell preparations, each derived from a different single donor using the HT12 V4 R2 bead chip arrays from Illumina.

Methods

Total RNA obtained from monocyte-derived macrophages exposed to representative polarizing cytokines

Additional Information
  • Martinez FO, Helming L, Milde R, Varin A et al. Genetic programs expressed in resting and IL-4 alternatively activated mouse and human macrophages: similarities and differences. Blood 2013 Feb 28;121(9):e57-69. PMID: 23293084
  • Platform Illumina HumanHT-12 v4
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