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Clinical

Similarities and differences between human activated macrophages -GSE49240

Purpose

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Hypothesis

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Experimental Design

The human AA dataset 5 includes the gene expression profile of monocyte-derived macrophages isolated from blood donor buffy coats and plated on TCP in medium containing 10% autologous serum. Cells were stimulated for 120 hours with 50 ng/ml of IL-4, 50 U/ml of IFN-? plus 12.5 ng/ml of TNF-?, 50 ng/ml of M-CSF or 50 ng/ml of IL-10. RNA samples were isolated using TriPure isolation reagent (Roche) and the RNeasy Mini Kit-Qiagen Clean up method. The transcriptional profile was evaluated in two independent cell preparations, each derived from a different single donor using the HT12 V4 R2 bead chip arrays from Illumina.

Experimental Variables

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Controls

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Methods

Total RNA obtained from monocyte-derived macrophages exposed to representative polarizing cytokines

Additional Information
  • Martinez FO, Helming L, Milde R, Varin A et al. Genetic programs expressed in resting and IL-4 alternatively activated mouse and human macrophages: similarities and differences. Blood 2013 Feb 28;121(9):e57-69. PMID: 23293084
  • Microarray
    Illumina HumanHT-12 v4
    12 Samples Loaded: 12
    Human (Homo sapiens)
    Monocyte-derived macrophages (MDMs) , Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title cell type culture condition
    GSM1195728 Monocytes (SAMPLE 1) monocytes Monocytes directly after isolation
    GSM1195729 Monocytes (SAMPLE 2) monocytes Monocytes directly after isolation
    GSM1195730 Medium (SAMPLE 3) monocyte-derived macrophages 10% Human serum
    GSM1195731 Medium (SAMPLE 4) monocyte-derived macrophages 10% Human serum
    GSM1195732 M-CSF (SAMPLE 5) monocyte-derived macrophages 10% Human serum+50 ng/ml of M-CSF
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    Log2 Transformed
    Raw Signal
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    Clinical Datasource
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    All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

    Sample ID !Sample Title Cell type Culture condition
    GSM1195728
    Monocytes (SAMPLE 1)
    monocytes
    Monocytes directly after isolation
    GSM1195729
    Monocytes (SAMPLE 2)
    monocytes
    Monocytes directly after isolation
    GSM1195730
    Medium (SAMPLE 3)
    monocyte-derived macrophages
    10% Human serum
    GSM1195731
    Medium (SAMPLE 4)
    monocyte-derived macrophages
    10% Human serum
    GSM1195732
    M-CSF (SAMPLE 5)
    monocyte-derived macrophages
    10% Human serum+50 ng/ml of M-CSF
    GSM1195733
    M-CSF (SAMPLE 6)
    monocyte-derived macrophages
    10% Human serum+50 ng/ml of M-CSF
    GSM1195734
    IL-10 (SAMPLE 7)
    monocyte-derived macrophages
    10% Human serum+50 ng/ml of IL-4
    GSM1195735
    IL-10 (SAMPLE 8)
    monocyte-derived macrophages
    10% Human serum+50 ng/ml of IL-4
    GSM1195736
    IL-4 (SAMPLE 9)
    monocyte-derived macrophages
    10% Human serum+50 ng/ml of IL-10
    GSM1195737
    IL-4 (SAMPLE 10)
    monocyte-derived macrophages
    10% Human serum+50 ng/ml of IL-10
    GSM1195738
    IFN-y (SAMPLE 11)
    monocyte-derived macrophages
    10% Human serum+50 U/ml of IFN-γ plus 12.5 ng/ml of TNF-α
    GSM1195739
    IFN-y (SAMPLE 12)
    monocyte-derived macrophages
    10% Human serum+50 U/ml of IFN-γ plus 12.5 ng/ml of TNF-α

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