Sample Set Annotation Tool: Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response-GSE8921

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Hypothesis

Experimental Design

The monocyte microarrays were generated using primary human monocytes stimulated with a TLR2/1L or media at 0, 3, 6, 12, 24 hour timepoints. A total of 10 donors were used with time 0 h samples prepared for each. For time points, we used cells from 4 of the 10 donors to prepare media and TLR2/1-stimulated samples.Dendritic cells were generated by culturing primary human monocytes with GM-CSF (800 U/ml) and IL-4 (1000 U/ml) for 7 days. The cells were then harvested and recultured for 12 hours with TLR2/1L or media. A total of four donors were used.

Experimental Variables

Controls

Methods

Additional Information

Liu PT, Stenger S, Li H, Wenzel L et al. Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response. Science 2006 Mar 24;311(5768):1770-3. PMID: 16497887

Expected Samples:

50 Samples Loaded: 50

Species

Human (Homo sapiens)

Sample Source

Monocytes , Monocyte-derived Dendritic cells (MDCs)

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	Primary human peripherial monocytes	time	Donor number
GSM209847	D08 0h Media	True	0	Donor 8
GSM225347	D08 12h Media	True	12	Donor 8
GSM225348	D08 12h TLR2/1L	True	12	Donor 8
GSM225349	D02 0h Media	True	0	Donor 2
GSM225350	D02 3h Media	True	3	Donor 2

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File
sampleset4000009_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	!Sample Title	Primary human peripherial monocytes	Human dendritic cells derived with GM-CSF and IL-4	Time	Donor number	Conditions
GSM209847	D08 0h Media	True		0	Donor 8	CD14 control 0 h
GSM225347	D08 12h Media	True		12	Donor 8	CD14 control 12 h
GSM225348	D08 12h TLR2/1L	True		12	Donor 8	CD14+TLR2/1L 12 h
GSM225349	D02 0h Media	True		0	Donor 2	CD14 control 0 h
GSM225350	D02 3h Media	True		3	Donor 2	CD14 control 3 h
GSM225351	D02 3h TLR2/1L	True		3	Donor 2	CD14+TLR2/1L 3 h
GSM225353	D05 0h Media	True		0	Donor 5	CD14 control 0 h
GSM225354	D05 6h Media	True		6	Donor 5	CD14 control 6 h
GSM225355	D05 6h TLR2/1L	True		6	Donor 5	CD14+TLR2/1L 6 h
GSM225356	D04 0h Media	True		0	Donor 4	CD14 control 0 h
GSM225357	D04 3h Media	True		3	Donor 4	CD14 control 3 h
GSM225358	D04 3h TLR2/1L	True		3	Donor 4	CD14+TLR2/1L 3 h
GSM225360	D06 6h Media	True		6	Donor 6	CD14 control 6 h
GSM225361	D06 6h TLR2/1L	True		6	Donor 6	CD14+TLR2/1L 6 h
GSM225364	D11 0h Media	True		0	Donor 11	CD14 control 0 h
GSM225365	D11 24h TLR2/1L	True		24	Donor 11	CD14+TLR2/1L 24 h
GSM225366	D11 24h Media	True		24	Donor 11	CD14 control 24 h
GSM225367	D03 0h Media	True		0	Donor 3	CD14 control 0 h
GSM225368	D03 3h Media	True		3	Donor 3	CD14 control 3 h
GSM225369	D03 3h TLR2/1L	True		3	Donor 3	CD14+TLR2/1L 3 h
GSM225370	D10 0h Media	True		0	Donor 10	CD14 control 0 h
GSM225372	D10 24h TLR2/1L	True		24	Donor 10	CD14+TLR2/1L 24 h
GSM225373	D10 24h Media	True		24	Donor 10	CD14 control 24 h
GSM225374	D01 0h Media	True		0	Donor 1	CD14 control 0 h
GSM225376	D01 12h TLR2/1L	True		12	Donor 1	CD14+TLR2/1L 12 h
GSM225377	D01 12h Media	True		12	Donor 1	CD14 control 12 h
GSM225378	D01 24h TLR2/1L	True		24	Donor 1	CD14+TLR2/1L 24 h
GSM225380	D01 3h TLR2/1L	True		3	Donor 1	CD14+TLR2/1L 3 h
GSM225645	D01 3h Media	True		3	Donor 1	CD14 control 3 h
GSM225649	D01 6h TLR2/1L	True		6	Donor 1	CD14+TLR2/1L 6 h
GSM225651	D01 6h Media	True		6	Donor 1	CD14 control 6 h
GSM225653	D07 0h Media	True		0	Donor 7	CD14 control 0 h
GSM225654	D07 12h TLR2/1L	True		12	Donor 7	CD14+TLR2/1L 12 h
GSM225655	D07 6h TLR2/1L	True		6	Donor 7	CD14+TLR2/1L 6 h
GSM225656	D07 12h Media	True		12	Donor 7	CD14 control 12 h
GSM225657	D07 6h Media	True		6	Donor 7	CD14 control 6 h
GSM225658	D09 0h Media	True		0	Donor 9	CD14 control 0 h
GSM225659	D09 12h TLR2/1L	True		12	Donor 9	CD14+TLR2/1L 12 h
GSM225660	D09 12h Media	True		12	Donor 9	CD14 control 12 h
GSM225661	D09 24h TLR2/1L	True		24	Donor 9	CD14+TLR2/1L 24 h
GSM225663	D09 24h Media	True		24	Donor 9	CD14 control 24 h
GSM225712	D01 24h Media	True		24	Donor 1	CD14 control 24 h
GSM225860	D12 12h media		True	12	Donor 12	CD14 control 12 h
GSM225867	D12 12h TLR2/1L		True	12	Donor 12	MDC+ TLR2/1L 12 h
GSM225871	D13 12h TLR2/1L		True	12	Donor 13	MDC+ TLR2/1L 12 h
GSM225875	D13 12h Media		True	12	Donor 13	MDC Control 12 h
GSM225878	D14 12h Media		True	12	Donor 14	MDC Control 12 h
GSM225881	D14 12h TLR2/1L		True	12	Donor 14	MDC+ TLR2/1L 12 h
GSM225885	D15 12h Media		True	12	Donor 15	MDC Control 12 h
GSM225887	D15 12h TLR2/1L		True	12	Donor 15	MDC+ TLR2/1L 12 h

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