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Clinical
GSE8921_family
Purpose

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Hypothesis

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Experimental Design

The monocyte microarrays were generated using primary human monocytes stimulated with a TLR2/1L or media at 0, 3, 6, 12, 24 hour timepoints. A total of 10 donors were used with time 0 h samples prepared for each. For time points, we used cells from 4 of the 10 donors to prepare media and TLR2/1-stimulated samples.Dendritic cells were generated by culturing primary human monocytes with GM-CSF (800 U/ml) and IL-4 (1000 U/ml) for 7 days. The cells were then harvested and recultured for 12 hours with TLR2/1L or media. A total of four donors were used.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Liu PT, Stenger S, Li H, Wenzel L et al. Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response. Science 2006 Mar 24;311(5768):1770-3. PMID: 16497887
  • Microarray
    Affymetrix HG-U133A
    50 Samples Loaded: 50
    Human (Homo sapiens)
    Monocyte-derived Dendritic cells (MDCs) , Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title Primary human peripherial monocytes Human dendritic cells derived with GM-CSF and IL-4 time Donor number
    GSM209847 D08 0h Media True 0 Donor 8
    GSM225347 D08 12h Media True 12 Donor 8
    GSM225348 D08 12h TLR2/1L True 12 Donor 8
    GSM225349 D02 0h Media True 0 Donor 2
    GSM225350 D02 3h Media True 3 Donor 2
    Please log in in order to upload files.
    File
    sampleset4000009_sampleannotations.csv
    Sample Set Spreadsheet
    Raw Signal
    Raw Signal
    •   |  View in GXB »
    Clinical Datasource
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    Samples Viewer / Editor

    All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

    Sample ID !Sample Title Primary human peripherial monocytes Human dendritic cells derived with GM-CSF and IL-4 Time Donor number Conditions
    GSM209847
    D08 0h Media
    True
     
    0
    Donor 8
    CD14 control 0 h
    GSM225347
    D08 12h Media
    True
     
    12
    Donor 8
    CD14 control 12 h
    GSM225348
    D08 12h TLR2/1L
    True
     
    12
    Donor 8
    CD14+TLR2/1L 12 h
    GSM225349
    D02 0h Media
    True
     
    0
    Donor 2
    CD14 control 0 h
    GSM225350
    D02 3h Media
    True
     
    3
    Donor 2
    CD14 control 3 h
    GSM225351
    D02 3h TLR2/1L
    True
     
    3
    Donor 2
    CD14+TLR2/1L 3 h
    GSM225353
    D05 0h Media
    True
     
    0
    Donor 5
    CD14 control 0 h
    GSM225354
    D05 6h Media
    True
     
    6
    Donor 5
    CD14 control 6 h
    GSM225355
    D05 6h TLR2/1L
    True
     
    6
    Donor 5
    CD14+TLR2/1L 6 h
    GSM225356
    D04 0h Media
    True
     
    0
    Donor 4
    CD14 control 0 h
    GSM225357
    D04 3h Media
    True
     
    3
    Donor 4
    CD14 control 3 h
    GSM225358
    D04 3h TLR2/1L
    True
     
    3
    Donor 4
    CD14+TLR2/1L 3 h
    GSM225360
    D06 6h Media
    True
     
    6
    Donor 6
    CD14 control 6 h
    GSM225361
    D06 6h TLR2/1L
    True
     
    6
    Donor 6
    CD14+TLR2/1L 6 h
    GSM225364
    D11 0h Media
    True
     
    0
    Donor 11
    CD14 control 0 h
    GSM225365
    D11 24h TLR2/1L
    True
     
    24
    Donor 11
    CD14+TLR2/1L 24 h
    GSM225366
    D11 24h Media
    True
     
    24
    Donor 11
    CD14 control 24 h
    GSM225367
    D03 0h Media
    True
     
    0
    Donor 3
    CD14 control 0 h
    GSM225368
    D03 3h Media
    True
     
    3
    Donor 3
    CD14 control 3 h
    GSM225369
    D03 3h TLR2/1L
    True
     
    3
    Donor 3
    CD14+TLR2/1L 3 h
    GSM225370
    D10 0h Media
    True
     
    0
    Donor 10
    CD14 control 0 h
    GSM225372
    D10 24h TLR2/1L
    True
     
    24
    Donor 10
    CD14+TLR2/1L 24 h
    GSM225373
    D10 24h Media
    True
     
    24
    Donor 10
    CD14 control 24 h
    GSM225374
    D01 0h Media
    True
     
    0
    Donor 1
    CD14 control 0 h
    GSM225376
    D01 12h TLR2/1L
    True
     
    12
    Donor 1
    CD14+TLR2/1L 12 h
    GSM225377
    D01 12h Media
    True
     
    12
    Donor 1
    CD14 control 12 h
    GSM225378
    D01 24h TLR2/1L
    True
     
    24
    Donor 1
    CD14+TLR2/1L 24 h
    GSM225380
    D01 3h TLR2/1L
    True
     
    3
    Donor 1
    CD14+TLR2/1L 3 h
    GSM225645
    D01 3h Media
    True
     
    3
    Donor 1
    CD14 control 3 h
    GSM225649
    D01 6h TLR2/1L
    True
     
    6
    Donor 1
    CD14+TLR2/1L 6 h
    GSM225651
    D01 6h Media
    True
     
    6
    Donor 1
    CD14 control 6 h
    GSM225653
    D07 0h Media
    True
     
    0
    Donor 7
    CD14 control 0 h
    GSM225654
    D07 12h TLR2/1L
    True
     
    12
    Donor 7
    CD14+TLR2/1L 12 h
    GSM225655
    D07 6h TLR2/1L
    True
     
    6
    Donor 7
    CD14+TLR2/1L 6 h
    GSM225656
    D07 12h Media
    True
     
    12
    Donor 7
    CD14 control 12 h
    GSM225657
    D07 6h Media
    True
     
    6
    Donor 7
    CD14 control 6 h
    GSM225658
    D09 0h Media
    True
     
    0
    Donor 9
    CD14 control 0 h
    GSM225659
    D09 12h TLR2/1L
    True
     
    12
    Donor 9
    CD14+TLR2/1L 12 h
    GSM225660
    D09 12h Media
    True
     
    12
    Donor 9
    CD14 control 12 h
    GSM225661
    D09 24h TLR2/1L
    True
     
    24
    Donor 9
    CD14+TLR2/1L 24 h
    GSM225663
    D09 24h Media
    True
     
    24
    Donor 9
    CD14 control 24 h
    GSM225712
    D01 24h Media
    True
     
    24
    Donor 1
    CD14 control 24 h
    GSM225860
    D12 12h media
     
    True
    12
    Donor 12
    CD14 control 12 h
    GSM225867
    D12 12h TLR2/1L
     
    True
    12
    Donor 12
    MDC+ TLR2/1L 12 h
    GSM225871
    D13 12h TLR2/1L
     
    True
    12
    Donor 13
    MDC+ TLR2/1L 12 h
    GSM225875
    D13 12h Media
     
    True
    12
    Donor 13
    MDC Control 12 h
    GSM225878
    D14 12h Media
     
    True
    12
    Donor 14
    MDC Control 12 h
    GSM225881
    D14 12h TLR2/1L
     
    True
    12
    Donor 14
    MDC+ TLR2/1L 12 h
    GSM225885
    D15 12h Media
     
    True
    12
    Donor 15
    MDC Control 12 h
    GSM225887
    D15 12h TLR2/1L
     
    True
    12
    Donor 15
    MDC+ TLR2/1L 12 h

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