Sample Set Annotation Tool: Human monocyte activation with NOD2L vs. TLR2/1L-GSE34156

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

In this study, we identified genes that were differentially expressed in human monocytes activated with eiter NOD2L and/or TLR2/1L.

Hypothesis

Experimental Design

Human blood monocytes were purified from healthy donors by Ficoll, Percoll and adherence. Monocytes were activated using NOD2L (MDP) and the TLR2/1L (19kD, triacylated peptide). Cells were harvested before activation (0h) and 6h and 24h after stimulation with ligands.

Experimental Variables

Controls

Methods

Additional Information

Schenk M, Krutzik SR, Sieling PA, Lee DJ et al. NOD2 triggers an interleukin-32-dependent human dendritic cell program in leprosy. Nat Med 2012 Mar 25;18(4):555-63. PMID: 22447076

Expected Samples:

45 Samples Loaded: 45

Species

Human (Homo sapiens)

Sample Source

Monocytes

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	cell type	tissue	Culture conditions
GSM843210	Donor 1 sample1	monocytes	healthy human blood	media t0h
GSM843211	Donor 1 sample2	monocytes	healthy human blood	media t6h
GSM843212	Donor 1 sample3	monocytes	healthy human blood	NOD2L t6h
GSM843213	Donor 1 sample4	monocytes	healthy human blood	TLR2_1L t6h
GSM843214	Donor 1 sample5	monocytes	healthy human blood	NOD2L+ TLR2_1L t6h

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File
sampleset4000118_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Cell type	Tissue	Culture conditions
GSM843210	Donor 1 sample1	monocytes	healthy human blood	media t0h
GSM843211	Donor 1 sample2	monocytes	healthy human blood	media t6h
GSM843212	Donor 1 sample3	monocytes	healthy human blood	NOD2L t6h
GSM843213	Donor 1 sample4	monocytes	healthy human blood	TLR2_1L t6h
GSM843214	Donor 1 sample5	monocytes	healthy human blood	NOD2L+ TLR2_1L t6h
GSM843215	Donor 1 sample6	monocytes	healthy human blood	media t24h
GSM843216	Donor 1 sample7	monocytes	healthy human blood	NOD2L t24h
GSM843217	Donor 1 sample8	monocytes	healthy human blood	TLR2_1L t24h
GSM843218	Donor 1 sample9	monocytes	healthy human blood	NOD2L+ TLR2_1L t24h
GSM843219	Donor 2 sample1	monocytes	healthy human blood	media t0h
GSM843220	Donor 2 sample2	monocytes	healthy human blood	media t6h
GSM843221	Donor 2 sample3	monocytes	healthy human blood	NOD2L t6h
GSM843222	Donor 2 sample4	monocytes	healthy human blood	TLR2_1L t6h
GSM843223	Donor 2 sample5	monocytes	healthy human blood	NOD2L+ TLR2_1L t6h
GSM843224	Donor 2 sample6	monocytes	healthy human blood	media t24h
GSM843225	Donor 2 sample7	monocytes	healthy human blood	NOD2L t24h
GSM843226	Donor 2 sample8	monocytes	healthy human blood	TLR2_1L t24h
GSM843227	Donor 2 sample9	monocytes	healthy human blood	NOD2L+ TLR2_1L t24h
GSM843228	Donor 3 sample1	monocytes	healthy human blood	media t0h
GSM843229	Donor 3 sample2	monocytes	healthy human blood	media t6h
GSM843230	Donor 3 sample3	monocytes	healthy human blood	NOD2L t6h
GSM843231	Donor 3 sample4	monocytes	healthy human blood	TLR2_1L t6h
GSM843232	Donor 3 sample5	monocytes	healthy human blood	NOD2L+ TLR2_1L t6h
GSM843233	Donor 3 sample6	monocytes	healthy human blood	media t24h
GSM843234	Donor 3 sample7	monocytes	healthy human blood	NOD2L t24h
GSM843235	Donor 3 sample8	monocytes	healthy human blood	TLR2_1L t24h
GSM843236	Donor 3 sample9	monocytes	healthy human blood	NOD2L+ TLR2_1L t24h
GSM843237	Donor 4 sample1	monocytes	healthy human blood	media t0h
GSM843238	Donor 4 sample2	monocytes	healthy human blood	media t6h
GSM843239	Donor 4 sample3	monocytes	healthy human blood	NOD2L t6h
GSM843240	Donor 4 sample4	monocytes	healthy human blood	TLR2_1L t6h
GSM843241	Donor 4 sample5	monocytes	healthy human blood	NOD2L+ TLR2_1L t6h
GSM843242	Donor 4 sample6	monocytes	healthy human blood	media t24h
GSM843243	Donor 4 sample7	monocytes	healthy human blood	NOD2L t24h
GSM843244	Donor 4 sample8	monocytes	healthy human blood	TLR2_1L t24h
GSM843245	Donor 4 sample9	monocytes	healthy human blood	NOD2L+ TLR2_1L t24h
GSM843246	Donor 5 sample1	monocytes	healthy human blood	media t0h
GSM843247	Donor 5 sample2	monocytes	healthy human blood	media t6h
GSM843248	Donor 5 sample3	monocytes	healthy human blood	NOD2L t6h
GSM843249	Donor 5 sample4	monocytes	healthy human blood	TLR2_1L t6h
GSM843250	Donor 5 sample5	monocytes	healthy human blood	NOD2L+ TLR2_1L t6h
GSM843251	Donor 5 sample6	monocytes	healthy human blood	media t24h
GSM843252	Donor 5 sample7	monocytes	healthy human blood	NOD2L t24h
GSM843253	Donor 5 sample8	monocytes	healthy human blood	TLR2_1L t24h
GSM843254	Donor 5 sample9	monocytes	healthy human blood	NOD2L+ TLR2_1L t24h

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Module Analysis

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