Human monocyte activation with NOD2L vs. TLR2/1L
In this study, we identified genes that were differentially expressed in human monocytes activated with eiter NOD2L and/or TLR2/1L.
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Human blood monocytes were purified from healthy donors by Ficoll, Percoll and adherence. Monocytes were activated using NOD2L (MDP) and the TLR2/1L (19kD, triacylated peptide). Cells were harvested before activation (0h) and 6h and 24h after stimulation with ligands.
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Sample ID | !Sample_title | cell type | tissue | Culture conditions |
---|---|---|---|---|
GSM843210 | Donor 1 sample1 | monocytes | healthy human blood | media t0h |
GSM843211 | Donor 1 sample2 | monocytes | healthy human blood | media t6h |
GSM843212 | Donor 1 sample3 | monocytes | healthy human blood | NOD2L t6h |
GSM843213 | Donor 1 sample4 | monocytes | healthy human blood | TLR2_1L t6h |
GSM843214 | Donor 1 sample5 | monocytes | healthy human blood | NOD2L+ TLR2_1L t6h |
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Sample ID | !Sample Title | Cell type | Tissue | Culture conditions |
---|---|---|---|---|
GSM843210 | Donor 1 sample1 |
monocytes |
healthy human blood |
media t0h |
GSM843211 | Donor 1 sample2 |
monocytes |
healthy human blood |
media t6h |
GSM843212 | Donor 1 sample3 |
monocytes |
healthy human blood |
NOD2L t6h |
GSM843213 | Donor 1 sample4 |
monocytes |
healthy human blood |
TLR2_1L t6h |
GSM843214 | Donor 1 sample5 |
monocytes |
healthy human blood |
NOD2L+ TLR2_1L t6h |
GSM843215 | Donor 1 sample6 |
monocytes |
healthy human blood |
media t24h |
GSM843216 | Donor 1 sample7 |
monocytes |
healthy human blood |
NOD2L t24h |
GSM843217 | Donor 1 sample8 |
monocytes |
healthy human blood |
TLR2_1L t24h |
GSM843218 | Donor 1 sample9 |
monocytes |
healthy human blood |
NOD2L+ TLR2_1L t24h |
GSM843219 | Donor 2 sample1 |
monocytes |
healthy human blood |
media t0h |
GSM843220 | Donor 2 sample2 |
monocytes |
healthy human blood |
media t6h |
GSM843221 | Donor 2 sample3 |
monocytes |
healthy human blood |
NOD2L t6h |
GSM843222 | Donor 2 sample4 |
monocytes |
healthy human blood |
TLR2_1L t6h |
GSM843223 | Donor 2 sample5 |
monocytes |
healthy human blood |
NOD2L+ TLR2_1L t6h |
GSM843224 | Donor 2 sample6 |
monocytes |
healthy human blood |
media t24h |
GSM843225 | Donor 2 sample7 |
monocytes |
healthy human blood |
NOD2L t24h |
GSM843226 | Donor 2 sample8 |
monocytes |
healthy human blood |
TLR2_1L t24h |
GSM843227 | Donor 2 sample9 |
monocytes |
healthy human blood |
NOD2L+ TLR2_1L t24h |
GSM843228 | Donor 3 sample1 |
monocytes |
healthy human blood |
media t0h |
GSM843229 | Donor 3 sample2 |
monocytes |
healthy human blood |
media t6h |
GSM843230 | Donor 3 sample3 |
monocytes |
healthy human blood |
NOD2L t6h |
GSM843231 | Donor 3 sample4 |
monocytes |
healthy human blood |
TLR2_1L t6h |
GSM843232 | Donor 3 sample5 |
monocytes |
healthy human blood |
NOD2L+ TLR2_1L t6h |
GSM843233 | Donor 3 sample6 |
monocytes |
healthy human blood |
media t24h |
GSM843234 | Donor 3 sample7 |
monocytes |
healthy human blood |
NOD2L t24h |
GSM843235 | Donor 3 sample8 |
monocytes |
healthy human blood |
TLR2_1L t24h |
GSM843236 | Donor 3 sample9 |
monocytes |
healthy human blood |
NOD2L+ TLR2_1L t24h |
GSM843237 | Donor 4 sample1 |
monocytes |
healthy human blood |
media t0h |
GSM843238 | Donor 4 sample2 |
monocytes |
healthy human blood |
media t6h |
GSM843239 | Donor 4 sample3 |
monocytes |
healthy human blood |
NOD2L t6h |
GSM843240 | Donor 4 sample4 |
monocytes |
healthy human blood |
TLR2_1L t6h |
GSM843241 | Donor 4 sample5 |
monocytes |
healthy human blood |
NOD2L+ TLR2_1L t6h |
GSM843242 | Donor 4 sample6 |
monocytes |
healthy human blood |
media t24h |
GSM843243 | Donor 4 sample7 |
monocytes |
healthy human blood |
NOD2L t24h |
GSM843244 | Donor 4 sample8 |
monocytes |
healthy human blood |
TLR2_1L t24h |
GSM843245 | Donor 4 sample9 |
monocytes |
healthy human blood |
NOD2L+ TLR2_1L t24h |
GSM843246 | Donor 5 sample1 |
monocytes |
healthy human blood |
media t0h |
GSM843247 | Donor 5 sample2 |
monocytes |
healthy human blood |
media t6h |
GSM843248 | Donor 5 sample3 |
monocytes |
healthy human blood |
NOD2L t6h |
GSM843249 | Donor 5 sample4 |
monocytes |
healthy human blood |
TLR2_1L t6h |
GSM843250 | Donor 5 sample5 |
monocytes |
healthy human blood |
NOD2L+ TLR2_1L t6h |
GSM843251 | Donor 5 sample6 |
monocytes |
healthy human blood |
media t24h |
GSM843252 | Donor 5 sample7 |
monocytes |
healthy human blood |
NOD2L t24h |
GSM843253 | Donor 5 sample8 |
monocytes |
healthy human blood |
TLR2_1L t24h |
GSM843254 | Donor 5 sample9 |
monocytes |
healthy human blood |
NOD2L+ TLR2_1L t24h |
Name | ||
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