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Clinical

CD4 on human monocytes -GSE32939

Purpose

We examined the efffects of ligating CD4 expressed by primary human peripheral blood monocytes with soluble MHC Class II.

Hypothesis

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Experimental Design

3 replicates are included. Fresh blood monocytes, obtained through negative selection via magnetic activted cell sorting (Miltenyi, Auburn, CA), from three separate individual donors were treated with RPMI-1640 medium alone or with medium containing sMHC II (1 ?g/ml) for three hours. Cells were harvested and RNA purified using the RNeasy procedure (Qiagen). RNA was then analyzed by the UCLA Clinical Microarray Core Laboratory using the Human U133 Plus 2.0 Array (Affimetrix). Data was analyzed using Dchip software. Visual inspection did not find any potential outliers or suspect areas. Data across all groups were normalized using the PM/MM difference.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Zhen A, Krutzik SR, Levin BR, Kasparian S et al. CD4 ligation on human blood monocytes triggers macrophage differentiation and enhances HIV infection. J Virol 2014 Sep 1;88(17):9934-46. PMID: 24942581
  • Microarray
    Affymetrix HG-U133_Plus_2
    6 Samples Loaded: 6
    Human (Homo sapiens)
    Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID Age !Sample_title treatment cell type
    GSM815833 adult TLR6 MEDIUM ONLY medium only Human peripheral blood monocytes
    GSM815834 adult TLR6 SMHC II sMHC II Human peripheral blood monocytes
    GSM815835 adult TLR7 MEDIUM ONLY medium only Human peripheral blood monocytes
    GSM815836 adult TLR7 SMHC II sMHC II Human peripheral blood monocytes
    GSM815837 adult TLR 17 MEDIUM ONLY medium only Human peripheral blood monocytes
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    Raw Signal
    Raw Signal
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    Sample ID Age !Sample Title Treatment Cell type
    GSM815833
    adult
    TLR6 MEDIUM ONLY
    medium only
    Human peripheral blood monocytes
    GSM815834
    adult
    TLR6 SMHC II
    sMHC II
    Human peripheral blood monocytes
    GSM815835
    adult
    TLR7 MEDIUM ONLY
    medium only
    Human peripheral blood monocytes
    GSM815836
    adult
    TLR7 SMHC II
    sMHC II
    Human peripheral blood monocytes
    GSM815837
    adult
    TLR 17 MEDIUM ONLY
    medium only
    Human peripheral blood monocytes
    GSM815838
    adult
    TLR17 SMHC II
    sMHC II
    Human peripheral blood monocytes

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