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Clinical

Genome-wide analysis of lupus immune complex stimulation of purified CD14+ monocytes and how this response is regulated by C1q-GSE32278

Purpose

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Hypothesis

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Experimental Design

Total RNA was isolated from CD14+ monocytes from 2 donors after 5 hours with the following conditions: 1) Unstimulated, 2) Lupus immune complex alone, 3) Lupus immune complex + C1q, 4) C1q alone

Experimental Variables

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Controls

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Methods

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Additional Information

Santer DM, Basom R, Elkon KB

Microarray
Illumina HumanRef-8 v3
8 Samples Loaded: 8
Human (Homo sapiens)
Monocytes
Healthy
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample_title donor treatment cell type
GSM799706 J1 unstimulated Donor 1 unstimulated CD14+
GSM799707 J2 immune complex Donor 1 immune complex CD14+
GSM799708 J3 immune complex + C1q Donor 1 immune complex + C1q CD14+
GSM799709 J4 C1q alone Donor 1 C1q CD14+
GSM799710 L1 unstimulated Donor 2 unstimulated CD14+
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Log2 Transformed
Raw Signal
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Sample ID !Sample Title Donor Treatment Cell type
GSM799706
J1 unstimulated
Donor 1
unstimulated
CD14+
GSM799707
J2 immune complex
Donor 1
immune complex
CD14+
GSM799708
J3 immune complex + C1q
Donor 1
immune complex + C1q
CD14+
GSM799709
J4 C1q alone
Donor 1
C1q
CD14+
GSM799710
L1 unstimulated
Donor 2
unstimulated
CD14+
GSM799711
L2 immune complex
Donor 2
immune complex
CD14+
GSM799712
L3 immune complex + C1q
Donor 2
immune complex + C1q
CD14+
GSM799713
L4 C1q alone
Donor 2
C1q
CD14+

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