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Clinical

cMyb and vMyb in human monocytes-GSE2816

Purpose

The transcriptional activities of c-Myb and its oncogenic variant v-Myb were compared by expressing them in primary human monocytes using recombinant adenovirus vectors. All the samples were compared to cells infected with a control adenovirus expressing only GFP. The results showed that v-Myb, which differs from c-Myb only by N- and C-terminal deletions and eleven amino acid substitutions, has a qualitatively different transcriptional activity.

Hypothesis

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Experimental Design

2 replicates of each type were analyzed. Replicates were performed independently, more than a week apart.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Liu F, Lei W, O'Rourke JP, Ness SA. Oncogenic mutations cause dramatic, qualitative changes in the transcriptional activity of c-Myb. Oncogene 2006 Feb 2;25(5):795-805. PMID: 16205643
  • Microarray
    Affymetrix HG-U133_Plus_2
    6 Samples Loaded: 6
    Human (Homo sapiens)
    Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title treatment Monocytes from buffy coat preparation
    GSM60924 control virus-infected replicate 1 (monocytes) Control True
    GSM60925 control virus-infected replicate 2 (monocytes) Control True
    GSM60926 cMyb virus-infected replicate 1 (monocytes) cMyb True
    GSM60927 cMyb virus-infected replicate 2 (monocytes) cMyb True
    GSM60928 vMyb virus-infected replicate 1 (monocytes) vMyb True
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    File
    sampleset4000111_sampleannotations.csv
    Sample Set Spreadsheet
    Raw Signal
    Raw Signal
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    All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

    Sample ID !Sample Title Treatment Monocytes from buffy coat preparation
    GSM60924
    control virus-infected replicate 1 (monocytes)
    Control
    True
    GSM60925
    control virus-infected replicate 2 (monocytes)
    Control
    True
    GSM60926
    cMyb virus-infected replicate 1 (monocytes)
    cMyb
    True
    GSM60927
    cMyb virus-infected replicate 2 (monocytes)
    cMyb
    True
    GSM60928
    vMyb virus-infected replicate 1 (monocytes)
    vMyb
    True
    GSM60929
    vMyb virus-infected replicate 2 (monocytes)
    vMyb
    True

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