Sample Set Annotation Tool: Cell Specific Expression in Trauma-Related Human T-Cell and Monocyte Pathways-GSE5580

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Monitoring genome-wide, cell-specific responses to human disease, although challenging, holds great promise for medicine’s future. Patients with injury severe enough to develop multiple organ dysfunction syndrome (MODS) are known to have multiple immune derangements, including T-cell apoptosis and anergy combined with depressed monocyte antigen presentation. Genome-wide expression analysis of highly-enriched circulating leukocyte subpopulations, combined with cell-specific pathway analyses, offers a previously unavailable opportunity to discover novel leukocyte regulatory networks in critically injured patients.

Hypothesis

Genome-wide expression analysis of highly-enriched circulating leukocyte subpopulations, combined with cell-specific pathway analyses, offers a previously unavailable opportunity to discover novel leukocyte regulatory networks in critically injured patients.

Experimental Design

Type of experiment: Gene expression profiling of circulating total bloodleukocytes, T-Cells, and Monocytes in severe trauma patients and healthy subjects.Experimental factors: Healthy subjects, 7 severely traumatized patientsand 7 healthy subjects for transcriptome analysis. Venous blood sampleswere collected. Total blood leukocytes were isolated, and T-cell andmonocyte populations were obtained from two subsequent aliquots of theleukocytes. Total leukocytes, enriched T-cells, and enriched monocytes were analyzed using Affymetrix GeneChip arrays.Number of hybridizations: 42 Human U133A GeneChip arrays (Affymetrix)

Experimental Variables

Controls

Age and gender-matched healthy controls were included in this study.

Methods

CD2+ CD3+ T cells were isolated, as well as CD14+ CD33+ monocytes.

Additional Information

Laudanski K, Miller-Graziano C, Xiao W, Mindrinos MN et al. Cell-specific expression and pathway analyses reveal alterations in trauma-related human T cell and monocyte pathways. Proc Natl Acad Sci U S A 2006 Oct 17;103(42):15564-9. PMID: 17032758

Expected Samples:

42 Samples Loaded: 42

Species

Human (Homo sapiens)

Sample Source

Leukocytes , T cells , Monocytes

Disease

Trauma

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	Gender	!Sample_title	Cell type	Disease Status
GSM129729	M	C1L	Leukocytes	Healthy Control
GSM129730	M	C1M	Monocytes	Healthy Control
GSM129731	M	C1T	T cells	Healthy Control
GSM129732	M	C2L	Leukocytes	Healthy Control
GSM129733	M	C2M	Monocytes	Healthy Control

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File
sampleset4000105_sampleannotations (1).csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	Age	Gender	!Sample Title	Cell type	Disease Status	Cell types	Human trauma patients with documented multiple organ failure and healthy volunteers	Day of draw MODS	Days Post Injury	Initial APACHE II	Initial ISS	Initial MODS	Patient ID
GSM129729		M	C1L	Leukocytes	Healthy Control	Healthy_Leukocyte	True						C1
GSM129730		M	C1M	Monocytes	Healthy Control	Healthy_Mono	True						C1
GSM129731		M	C1T	T cells	Healthy Control	Healthy_T cells	True						C1
GSM129732		M	C2L	Leukocytes	Healthy Control	Healthy_Leukocyte	True						C2
GSM129733		M	C2M	Monocytes	Healthy Control	Healthy_Mono	True						C2
GSM129734		M	C2T	T cells	Healthy Control	Healthy_T cells	True						C2
GSM129735		M	C3L	Leukocytes	Healthy Control	Healthy_Leukocyte	True						C3
GSM129736		M	C3M	Monocytes	Healthy Control	Healthy_Mono	True						C3
GSM129737		M	C3T	T cells	Healthy Control	Healthy_T cells	True						C3
GSM129738		M	C4L	Leukocytes	Healthy Control	Healthy_Leukocyte	True						C4
GSM129739		M	C4M	Monocytes	Healthy Control	Healthy_Mono	True						C4
GSM129740		M	C4T	T cells	Healthy Control	Healthy_T cells	True						C4
GSM129741		M	C5L	Leukocytes	Healthy Control	Healthy_Leukocyte	True						C5
GSM129742		M	C5M	Monocytes	Healthy Control	Healthy_Mono	True						C5
GSM129743		M	C5T	T cells	Healthy Control	Healthy_T cells	True						C5
GSM129744		M	C6L	Leukocytes	Healthy Control	Healthy_Leukocyte	True						C6
GSM129745		M	C6M	Monocytes	Healthy Control	Healthy_Mono	True						C6
GSM129746		M	C6T	T cells	Healthy Control	Healthy_T cells	True						C6
GSM129747		M	C7L	Leukocytes	Healthy Control	Healthy_Leukocyte	True						C7
GSM129748		M	C7M	Monocytes	Healthy Control	Healthy_Mono	True						C7
GSM129749		M	C7T	T cells	Healthy Control	Healthy_T cells	True						C7
GSM129750	75	M	P1L	Leukocytes	Trauma Patient	Trauma_Leukocyte	True	6	14	25	17	7	P1
GSM129751	75	M	P1M	Monocytes	Trauma Patient	Trauma_Mono	True	6	14	25	17	7	P1
GSM129752	75	M	P1T	T cells	Trauma Patient	Trauma_T cells	True	6	14	25	17	7	P1
GSM129753	19	M	P2L	Leukocytes	Trauma Patient	Trauma_Leukocyte	True	7	5	22	34	6	P2
GSM129754	19	M	P2M	Monocytes	Trauma Patient	Trauma_Mono	True	7	5	22	34	6	P2
GSM129755	19	M	P2T	T cells	Trauma Patient	Trauma_T cells	True	7	5	22	34	6	P2
GSM129756	74	M	P3L	Leukocytes	Trauma Patient	Trauma_Leukocyte	True	9	10	26	41	16	P3
GSM129757	74	M	P3M	Monocytes	Trauma Patient	Trauma_Mono	True	9	10	26	41	16	P3
GSM129758	74	M	P3T	T cells	Trauma Patient	Trauma_T cells	True	9	10	26	41	16	P3
GSM129759	42	M	P4L	Leukocytes	Trauma Patient	Trauma_Leukocyte	True	5	12	15	38	9	P4
GSM129760	42	M	P4M	Monocytes	Trauma Patient	Trauma_Mono	True	5	12	15	38	9	P4
GSM129761	42	M	P4T	T cells	Trauma Patient	Trauma_T cells	True	5	12	15	38	9	P4
GSM129762	36	M	P5L	Leukocytes	Trauma Patient	Trauma_Leukocyte	True	14	4	29	50	14	P5
GSM129763	36	M	P5M	Monocytes	Trauma Patient	Trauma_Mono	True	14	4	29	50	14	P5
GSM129764	36	M	P5T	T cells	Trauma Patient	Trauma_T cells	True	14	4	29	50	14	P5
GSM129765	49	M	P6L	Leukocytes	Trauma Patient	Trauma_Leukocyte	True	13	14	24	59	13	P6
GSM129766	49	M	P6M	Monocytes	Trauma Patient	Trauma_Mono	True	13	14	24	59	13	P6
GSM129767	49	M	P6T	T cells	Trauma Patient	Trauma_T cells	True	13	14	24	59	13	P6
GSM129768	38	M	P7L	Leukocytes	Trauma Patient	Trauma_Leukocyte	True	8	10	17	48	4	P7
GSM129769	38	M	P7M	Monocytes	Trauma Patient	Trauma_Mono	True	8	10	17	48	4	P7
GSM129770	38	M	P7T	T cells	Trauma Patient	Trauma_T cells	True	8	10	17	48	4	P7

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