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Clinical

Cell Specific Expression & Pathway Analyses Reveal Novel Alterations in Trauma-Related Human T-Cell & Monocyte Pathways-GSE5580

Purpose

Monitoring genome-wide, cell-specific responses to human disease, although challenging, holds great promise for medicine’s future. Patients with injury severe enough to develop multiple organ dysfunction syndrome (MODS) are known to have multiple immune derangements, including T-cell apoptosis and anergy combined with depressed monocyte antigen presentation. Genome-wide expression analysis of highly-enriched circulating leukocyte subpopulations, combined with cell-specific pathway analyses, offers a previously unavailable opportunity to discover novel leukocyte regulatory networks in critically injured patients.

Hypothesis

Genome-wide expression analysis of highly-enriched circulating leukocyte subpopulations, combined with cell-specific pathway analyses, offers a previously unavailable opportunity to discover novel leukocyte regulatory networks in critically injured patients.

Experimental Design

Type of experiment: Gene expression profiling of circulating total bloodleukocytes, T-Cells, and Monocytes in severe trauma patients and healthy subjects.Experimental factors: Healthy subjects, 7 severely traumatized patientsand 7 healthy subjects for transcriptome analysis. Venous blood sampleswere collected. Total blood leukocytes were isolated, and T-cell andmonocyte populations were obtained from two subsequent aliquots of theleukocytes. Total leukocytes, enriched T-cells, and enriched monocytes were analyzed using Affymetrix GeneChip arrays.Number of hybridizations: 42 Human U133A GeneChip arrays (Affymetrix)

Experimental Variables

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Controls

Age and gender-matched healthy controls were included in this study.

Methods

CD2+ CD3+ T cells were isolated, as well as CD14+ CD33+ monocytes.

Additional Information
  • Laudanski K, Miller-Graziano C, Xiao W, Mindrinos MN et al. Cell-specific expression and pathway analyses reveal alterations in trauma-related human T cell and monocyte pathways. Proc Natl Acad Sci U S A 2006 Oct 17;103(42):15564-9. PMID: 17032758
  • Microarray
    Affymetrix HG-U133A
    42 Samples Loaded: 42
    Human (Homo sapiens)
    T cells , Leukocytes , Monocytes
    Trauma
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID Age Gender !Sample_title Cell type Disease Status
    GSM129729 M C1L Leukocytes Healthy Control
    GSM129730 M C1M Monocytes Healthy Control
    GSM129731 M C1T T cells Healthy Control
    GSM129732 M C2L Leukocytes Healthy Control
    GSM129733 M C2M Monocytes Healthy Control
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    File
    sampleset4000105_sampleannotations (1).csv
    Sample Set Spreadsheet
    Raw Signal
    Raw Signal
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    Sample ID Age Gender !Sample Title Cell type Disease Status Cell types Human trauma patients with documented multiple organ failure and healthy volunteers Day of draw MODS Days Post Injury Initial APACHE II Initial ISS Initial MODS Patient ID
    GSM129729
     
    M
    C1L
    Leukocytes
    Healthy Control
    Healthy_Leukocyte
    True
     
     
     
     
     
    C1
    GSM129730
     
    M
    C1M
    Monocytes
    Healthy Control
    Healthy_Mono
    True
     
     
     
     
     
    C1
    GSM129731
     
    M
    C1T
    T cells
    Healthy Control
    Healthy_T cells
    True
     
     
     
     
     
    C1
    GSM129732
     
    M
    C2L
    Leukocytes
    Healthy Control
    Healthy_Leukocyte
    True
     
     
     
     
     
    C2
    GSM129733
     
    M
    C2M
    Monocytes
    Healthy Control
    Healthy_Mono
    True
     
     
     
     
     
    C2
    GSM129734
     
    M
    C2T
    T cells
    Healthy Control
    Healthy_T cells
    True
     
     
     
     
     
    C2
    GSM129735
     
    M
    C3L
    Leukocytes
    Healthy Control
    Healthy_Leukocyte
    True
     
     
     
     
     
    C3
    GSM129736
     
    M
    C3M
    Monocytes
    Healthy Control
    Healthy_Mono
    True
     
     
     
     
     
    C3
    GSM129737
     
    M
    C3T
    T cells
    Healthy Control
    Healthy_T cells
    True
     
     
     
     
     
    C3
    GSM129738
     
    M
    C4L
    Leukocytes
    Healthy Control
    Healthy_Leukocyte
    True
     
     
     
     
     
    C4
    GSM129739
     
    M
    C4M
    Monocytes
    Healthy Control
    Healthy_Mono
    True
     
     
     
     
     
    C4
    GSM129740
     
    M
    C4T
    T cells
    Healthy Control
    Healthy_T cells
    True
     
     
     
     
     
    C4
    GSM129741
     
    M
    C5L
    Leukocytes
    Healthy Control
    Healthy_Leukocyte
    True
     
     
     
     
     
    C5
    GSM129742
     
    M
    C5M
    Monocytes
    Healthy Control
    Healthy_Mono
    True
     
     
     
     
     
    C5
    GSM129743
     
    M
    C5T
    T cells
    Healthy Control
    Healthy_T cells
    True
     
     
     
     
     
    C5
    GSM129744
     
    M
    C6L
    Leukocytes
    Healthy Control
    Healthy_Leukocyte
    True
     
     
     
     
     
    C6
    GSM129745
     
    M
    C6M
    Monocytes
    Healthy Control
    Healthy_Mono
    True
     
     
     
     
     
    C6
    GSM129746
     
    M
    C6T
    T cells
    Healthy Control
    Healthy_T cells
    True
     
     
     
     
     
    C6
    GSM129747
     
    M
    C7L
    Leukocytes
    Healthy Control
    Healthy_Leukocyte
    True
     
     
     
     
     
    C7
    GSM129748
     
    M
    C7M
    Monocytes
    Healthy Control
    Healthy_Mono
    True
     
     
     
     
     
    C7
    GSM129749
     
    M
    C7T
    T cells
    Healthy Control
    Healthy_T cells
    True
     
     
     
     
     
    C7
    GSM129750
    75
    M
    P1L
    Leukocytes
    Trauma Patient
    Trauma_Leukocyte
    True
    6
    14
    25
    17
    7
    P1
    GSM129751
    75
    M
    P1M
    Monocytes
    Trauma Patient
    Trauma_Mono
    True
    6
    14
    25
    17
    7
    P1
    GSM129752
    75
    M
    P1T
    T cells
    Trauma Patient
    Trauma_T cells
    True
    6
    14
    25
    17
    7
    P1
    GSM129753
    19
    M
    P2L
    Leukocytes
    Trauma Patient
    Trauma_Leukocyte
    True
    7
    5
    22
    34
    6
    P2
    GSM129754
    19
    M
    P2M
    Monocytes
    Trauma Patient
    Trauma_Mono
    True
    7
    5
    22
    34
    6
    P2
    GSM129755
    19
    M
    P2T
    T cells
    Trauma Patient
    Trauma_T cells
    True
    7
    5
    22
    34
    6
    P2
    GSM129756
    74
    M
    P3L
    Leukocytes
    Trauma Patient
    Trauma_Leukocyte
    True
    9
    10
    26
    41
    16
    P3
    GSM129757
    74
    M
    P3M
    Monocytes
    Trauma Patient
    Trauma_Mono
    True
    9
    10
    26
    41
    16
    P3
    GSM129758
    74
    M
    P3T
    T cells
    Trauma Patient
    Trauma_T cells
    True
    9
    10
    26
    41
    16
    P3
    GSM129759
    42
    M
    P4L
    Leukocytes
    Trauma Patient
    Trauma_Leukocyte
    True
    5
    12
    15
    38
    9
    P4
    GSM129760
    42
    M
    P4M
    Monocytes
    Trauma Patient
    Trauma_Mono
    True
    5
    12
    15
    38
    9
    P4
    GSM129761
    42
    M
    P4T
    T cells
    Trauma Patient
    Trauma_T cells
    True
    5
    12
    15
    38
    9
    P4
    GSM129762
    36
    M
    P5L
    Leukocytes
    Trauma Patient
    Trauma_Leukocyte
    True
    14
    4
    29
    50
    14
    P5
    GSM129763
    36
    M
    P5M
    Monocytes
    Trauma Patient
    Trauma_Mono
    True
    14
    4
    29
    50
    14
    P5
    GSM129764
    36
    M
    P5T
    T cells
    Trauma Patient
    Trauma_T cells
    True
    14
    4
    29
    50
    14
    P5
    GSM129765
    49
    M
    P6L
    Leukocytes
    Trauma Patient
    Trauma_Leukocyte
    True
    13
    14
    24
    59
    13
    P6
    GSM129766
    49
    M
    P6M
    Monocytes
    Trauma Patient
    Trauma_Mono
    True
    13
    14
    24
    59
    13
    P6
    GSM129767
    49
    M
    P6T
    T cells
    Trauma Patient
    Trauma_T cells
    True
    13
    14
    24
    59
    13
    P6
    GSM129768
    38
    M
    P7L
    Leukocytes
    Trauma Patient
    Trauma_Leukocyte
    True
    8
    10
    17
    48
    4
    P7
    GSM129769
    38
    M
    P7M
    Monocytes
    Trauma Patient
    Trauma_Mono
    True
    8
    10
    17
    48
    4
    P7
    GSM129770
    38
    M
    P7T
    T cells
    Trauma Patient
    Trauma_T cells
    True
    8
    10
    17
    48
    4
    P7

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