Cell Specific Expression & Pathway Analyses Reveal Novel Alterations in Trauma-Related Human T-Cell & Monocyte Pathways-GSE5580
Monitoring genome-wide, cell-specific responses to human disease, although challenging, holds great promise for medicine’s future. Patients with injury severe enough to develop multiple organ dysfunction syndrome (MODS) are known to have multiple immune derangements, including T-cell apoptosis and anergy combined with depressed monocyte antigen presentation. Genome-wide expression analysis of highly-enriched circulating leukocyte subpopulations, combined with cell-specific pathway analyses, offers a previously unavailable opportunity to discover novel leukocyte regulatory networks in critically injured patients.
Genome-wide expression analysis of highly-enriched circulating leukocyte subpopulations, combined with cell-specific pathway analyses, offers a previously unavailable opportunity to discover novel leukocyte regulatory networks in critically injured patients.
Type of experiment: Gene expression profiling of circulating total bloodleukocytes, T-Cells, and Monocytes in severe trauma patients and healthy subjects.Experimental factors: Healthy subjects, 7 severely traumatized patientsand 7 healthy subjects for transcriptome analysis. Venous blood sampleswere collected. Total blood leukocytes were isolated, and T-cell andmonocyte populations were obtained from two subsequent aliquots of theleukocytes. Total leukocytes, enriched T-cells, and enriched monocytes were analyzed using Affymetrix GeneChip arrays.Number of hybridizations: 42 Human U133A GeneChip arrays (Affymetrix)
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Age and gender-matched healthy controls were included in this study.
CD2+ CD3+ T cells were isolated, as well as CD14+ CD33+ monocytes.
Sample ID | Age | Gender | !Sample_title | Cell type | Disease Status |
---|---|---|---|---|---|
GSM129729 | M | C1L | Leukocytes | Healthy Control | |
GSM129730 | M | C1M | Monocytes | Healthy Control | |
GSM129731 | M | C1T | T cells | Healthy Control | |
GSM129732 | M | C2L | Leukocytes | Healthy Control | |
GSM129733 | M | C2M | Monocytes | Healthy Control |
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Sample ID | Age | Gender | !Sample Title | Cell type | Disease Status | Cell types | Human trauma patients with documented multiple organ failure and healthy volunteers | Day of draw MODS | Days Post Injury | Initial APACHE II | Initial ISS | Initial MODS | Patient ID |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
GSM129729 | M |
C1L |
Leukocytes |
Healthy Control |
Healthy_Leukocyte |
True |
C1 |
||||||
GSM129730 | M |
C1M |
Monocytes |
Healthy Control |
Healthy_Mono |
True |
C1 |
||||||
GSM129731 | M |
C1T |
T cells |
Healthy Control |
Healthy_T cells |
True |
C1 |
||||||
GSM129732 | M |
C2L |
Leukocytes |
Healthy Control |
Healthy_Leukocyte |
True |
C2 |
||||||
GSM129733 | M |
C2M |
Monocytes |
Healthy Control |
Healthy_Mono |
True |
C2 |
||||||
GSM129734 | M |
C2T |
T cells |
Healthy Control |
Healthy_T cells |
True |
C2 |
||||||
GSM129735 | M |
C3L |
Leukocytes |
Healthy Control |
Healthy_Leukocyte |
True |
C3 |
||||||
GSM129736 | M |
C3M |
Monocytes |
Healthy Control |
Healthy_Mono |
True |
C3 |
||||||
GSM129737 | M |
C3T |
T cells |
Healthy Control |
Healthy_T cells |
True |
C3 |
||||||
GSM129738 | M |
C4L |
Leukocytes |
Healthy Control |
Healthy_Leukocyte |
True |
C4 |
||||||
GSM129739 | M |
C4M |
Monocytes |
Healthy Control |
Healthy_Mono |
True |
C4 |
||||||
GSM129740 | M |
C4T |
T cells |
Healthy Control |
Healthy_T cells |
True |
C4 |
||||||
GSM129741 | M |
C5L |
Leukocytes |
Healthy Control |
Healthy_Leukocyte |
True |
C5 |
||||||
GSM129742 | M |
C5M |
Monocytes |
Healthy Control |
Healthy_Mono |
True |
C5 |
||||||
GSM129743 | M |
C5T |
T cells |
Healthy Control |
Healthy_T cells |
True |
C5 |
||||||
GSM129744 | M |
C6L |
Leukocytes |
Healthy Control |
Healthy_Leukocyte |
True |
C6 |
||||||
GSM129745 | M |
C6M |
Monocytes |
Healthy Control |
Healthy_Mono |
True |
C6 |
||||||
GSM129746 | M |
C6T |
T cells |
Healthy Control |
Healthy_T cells |
True |
C6 |
||||||
GSM129747 | M |
C7L |
Leukocytes |
Healthy Control |
Healthy_Leukocyte |
True |
C7 |
||||||
GSM129748 | M |
C7M |
Monocytes |
Healthy Control |
Healthy_Mono |
True |
C7 |
||||||
GSM129749 | M |
C7T |
T cells |
Healthy Control |
Healthy_T cells |
True |
C7 |
||||||
GSM129750 | 75 |
M |
P1L |
Leukocytes |
Trauma Patient |
Trauma_Leukocyte |
True |
6 |
14 |
25 |
17 |
7 |
P1 |
GSM129751 | 75 |
M |
P1M |
Monocytes |
Trauma Patient |
Trauma_Mono |
True |
6 |
14 |
25 |
17 |
7 |
P1 |
GSM129752 | 75 |
M |
P1T |
T cells |
Trauma Patient |
Trauma_T cells |
True |
6 |
14 |
25 |
17 |
7 |
P1 |
GSM129753 | 19 |
M |
P2L |
Leukocytes |
Trauma Patient |
Trauma_Leukocyte |
True |
7 |
5 |
22 |
34 |
6 |
P2 |
GSM129754 | 19 |
M |
P2M |
Monocytes |
Trauma Patient |
Trauma_Mono |
True |
7 |
5 |
22 |
34 |
6 |
P2 |
GSM129755 | 19 |
M |
P2T |
T cells |
Trauma Patient |
Trauma_T cells |
True |
7 |
5 |
22 |
34 |
6 |
P2 |
GSM129756 | 74 |
M |
P3L |
Leukocytes |
Trauma Patient |
Trauma_Leukocyte |
True |
9 |
10 |
26 |
41 |
16 |
P3 |
GSM129757 | 74 |
M |
P3M |
Monocytes |
Trauma Patient |
Trauma_Mono |
True |
9 |
10 |
26 |
41 |
16 |
P3 |
GSM129758 | 74 |
M |
P3T |
T cells |
Trauma Patient |
Trauma_T cells |
True |
9 |
10 |
26 |
41 |
16 |
P3 |
GSM129759 | 42 |
M |
P4L |
Leukocytes |
Trauma Patient |
Trauma_Leukocyte |
True |
5 |
12 |
15 |
38 |
9 |
P4 |
GSM129760 | 42 |
M |
P4M |
Monocytes |
Trauma Patient |
Trauma_Mono |
True |
5 |
12 |
15 |
38 |
9 |
P4 |
GSM129761 | 42 |
M |
P4T |
T cells |
Trauma Patient |
Trauma_T cells |
True |
5 |
12 |
15 |
38 |
9 |
P4 |
GSM129762 | 36 |
M |
P5L |
Leukocytes |
Trauma Patient |
Trauma_Leukocyte |
True |
14 |
4 |
29 |
50 |
14 |
P5 |
GSM129763 | 36 |
M |
P5M |
Monocytes |
Trauma Patient |
Trauma_Mono |
True |
14 |
4 |
29 |
50 |
14 |
P5 |
GSM129764 | 36 |
M |
P5T |
T cells |
Trauma Patient |
Trauma_T cells |
True |
14 |
4 |
29 |
50 |
14 |
P5 |
GSM129765 | 49 |
M |
P6L |
Leukocytes |
Trauma Patient |
Trauma_Leukocyte |
True |
13 |
14 |
24 |
59 |
13 |
P6 |
GSM129766 | 49 |
M |
P6M |
Monocytes |
Trauma Patient |
Trauma_Mono |
True |
13 |
14 |
24 |
59 |
13 |
P6 |
GSM129767 | 49 |
M |
P6T |
T cells |
Trauma Patient |
Trauma_T cells |
True |
13 |
14 |
24 |
59 |
13 |
P6 |
GSM129768 | 38 |
M |
P7L |
Leukocytes |
Trauma Patient |
Trauma_Leukocyte |
True |
8 |
10 |
17 |
48 |
4 |
P7 |
GSM129769 | 38 |
M |
P7M |
Monocytes |
Trauma Patient |
Trauma_Mono |
True |
8 |
10 |
17 |
48 |
4 |
P7 |
GSM129770 | 38 |
M |
P7T |
T cells |
Trauma Patient |
Trauma_T cells |
True |
8 |
10 |
17 |
48 |
4 |
P7 |
Name | ||
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