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Transcriptome analysis of blood monocytes from sepsis patients-GSE46955

In Progress
Clinical

Transcriptome analysis of blood monocytes from sepsis patients-GSE46955

Purpose

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Hypothesis

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Experimental Design

Blood monocytes from gram-negative sepsis patients during sepsis (Sepsis) and following their recovery (Recovery/Basal) as well as healthy donor (control) were isolated. Thereafter, these cells were treated ex vivo with or without LPS for 3h and analysed for transcriptomic study.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Shalova IN, Lim JY, Chittezhath M, Zinkernagel AS et al. Human monocytes undergo functional re-programming during sepsis mediated by hypoxia-inducible factor-1?. Immunity 2015 Mar 17;42(3):484-98. PMID: 25746953
    • Microarray
    Illumina HumanRef-8 v2
    44 Samples Loaded: 44
    Human (Homo sapiens)
    Monocytes
    Sepsis
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title treatment cell Culture condition
    GSM1141941 monocyte-recovery-basal-1 None monocyte Recovery_baseline
    GSM1141942 monocyte-recovery-lps-1 LPS monocyte Recovery_LPS
    GSM1141943 monocyte-healthy-basal-1 None monocyte Healthy_baseline
    GSM1141944 monocyte-healthy-lps-1 LPS monocyte Healthy_LPS
    GSM1141945 monocyte-sepsis-basal-1 None monocyte Sepsis_baseline

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    Browse Files

    File
    sampleset4000098_sampleannotations.csv
    Sample Set Spreadsheet
    Log2 Transformed
    Raw Signal
    •   |  View in GXB »
    Clinical Datasource
    Links
    http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE46955
    http://www.ncbi.nlm.nih.gov/pubmed/25746953

    Samples Viewer / Editor

    All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

    Sample ID !Sample Title Treatment Cell Culture condition
    GSM1141941
    monocyte-recovery-basal-1
    None
    monocyte
    Recovery_baseline
    GSM1141942
    monocyte-recovery-lps-1
    LPS
    monocyte
    Recovery_LPS
    GSM1141943
    monocyte-healthy-basal-1
    None
    monocyte
    Healthy_baseline
    GSM1141944
    monocyte-healthy-lps-1
    LPS
    monocyte
    Healthy_LPS
    GSM1141945
    monocyte-sepsis-basal-1
    None
    monocyte
    Sepsis_baseline
    GSM1141946
    monocyte-sepsis-lps-1
    LPS
    monocyte
    Sepsis_LPS
    GSM1141947
    monocyte-recovery-basal-2
    None
    monocyte
    Recovery_baseline
    GSM1141948
    monocyte-recovery-lps-2
    LPS
    monocyte
    Recovery_LPS
    GSM1141949
    monocyte-healthy-basal-2
    None
    monocyte
    Healthy_baseline
    GSM1141950
    monocyte-healthy-lps-2
    LPS
    monocyte
    Healthy_LPS
    GSM1141951
    monocyte-sepsis-basal-2
    None
    monocyte
    Sepsis_baseline
    GSM1141952
    monocyte-sepsis-lps-2
    LPS
    monocyte
    Sepsis_LPS
    GSM1141953
    monocyte-recovery-basal-3
    None
    monocyte
    Recovery_baseline
    GSM1141954
    monocyte-recovery-lps-3
    LPS
    monocyte
    Recovery_LPS
    GSM1141955
    monocyte-healthy-basal-3
    None
    monocyte
    Healthy_baseline
    GSM1141956
    monocyte-healthy-lps-3
    LPS
    monocyte
    Healthy_LPS
    GSM1141957
    monocyte-sepsis-basal-3
    None
    monocyte
    Sepsis_baseline
    GSM1141958
    monocyte-sepsis-lps-3
    LPS
    monocyte
    Sepsis_LPS
    GSM1141959
    monocyte-healthy-basal-4
    None
    monocyte
    Healthy_baseline
    GSM1141960
    monocyte-healthy-lps-4
    LPS
    monocyte
    Healthy_LPS
    GSM1141961
    monocyte-healthy-basal-5
    None
    monocyte
    Healthy_baseline
    GSM1141962
    monocyte-healthy-lps-5
    LPS
    monocyte
    Healthy_LPS
    GSM1141963
    monocyte-healthy-basal-6
    None
    monocyte
    Healthy_baseline
    GSM1141964
    monocyte-healthy-lps-6
    LPS
    monocyte
    Healthy_LPS
    GSM1141965
    monocyte-recovery-basal-4
    None
    monocyte
    Recovery_baseline
    GSM1141966
    monocyte-recovery-lps-4
    LPS
    monocyte
    Recovery_LPS
    GSM1141967
    monocyte-sepsis-basal-4
    None
    monocyte
    Sepsis_baseline
    GSM1141968
    monocyte-sepsis-lps-4
    LPS
    monocyte
    Sepsis_LPS
    GSM1141969
    monocyte-recovery-basal-5
    None
    monocyte
    Recovery_baseline
    GSM1141970
    monocyte-recovery-lps-5
    LPS
    monocyte
    Recovery_LPS
    GSM1141971
    monocyte-sepsis-basal-5
    None
    monocyte
    Sepsis_baseline
    GSM1141972
    monocyte-sepsis-lps-5
    LPS
    monocyte
    Sepsis_LPS
    GSM1141973
    monocyte-recovery-basal-6
    None
    monocyte
    Recovery_baseline
    GSM1141974
    monocyte-recovery-lps-6
    LPS
    monocyte
    Recovery_LPS
    GSM1141975
    monocyte-sepsis-basal-6
    None
    monocyte
    Sepsis_baseline
    GSM1141976
    monocyte-sepsis-lps-6
    LPS
    monocyte
    Sepsis_LPS
    GSM1141977
    monocyte-recovery-basal-7
    None
    monocyte
    Recovery_baseline
    GSM1141978
    monocyte-recovery-lps-7
    LPS
    monocyte
    Recovery_LPS
    GSM1141979
    monocyte-sepsis-basal-7
    None
    monocyte
    Sepsis_baseline
    GSM1141980
    monocyte-sepsis-lps-7
    LPS
    monocyte
    Sepsis_LPS
    GSM1141981
    monocyte-recovery-basal-8
    None
    monocyte
    Recovery_baseline
    GSM1141982
    monocyte-recovery-lps-8
    LPS
    monocyte
    Recovery_LPS
    GSM1141983
    monocyte-sepsis-basal-8
    None
    monocyte
    Sepsis_baseline
    GSM1141984
    monocyte-sepsis-lps-8
    LPS
    monocyte
    Sepsis_LPS

    Group Sets View in Gene Expression Browser

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