Sample Set Annotation Tool: Gene expression profile of human monocytes stimulated with all-trans retinoic acid (ATRA) or 1,25a-dihydroxyvitamin D3 (1,25D3)-GSE46268

Study Design
Assay
Sample Info
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Samples
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Analysis

Purpose

A role for vitamin A in host defense against Mycobacterium tuberculosis has been suggested through epidemiological and in vitro studies; however, the antimicrobial mechanism is unclear. Here, we demonstrate that vitamin A mediates host defense through regulation of cellular cholesterol content.

Hypothesis

Experimental Design

Monocytes derived from four independent healthy blood donors that were stimulated with control (CTRL), ATRA or 1,25D3 at 10-8M for 18 hours.

Experimental Variables

Controls

Methods

Additional Information

Wheelwright M, Kim EW, Inkeles MS, De Leon A et al. All-trans retinoic acid-triggered antimicrobial activity against Mycobacterium tuberculosis is dependent on NPC2. J Immunol 2014 Mar 1;192(5):2280-90. PMID: 24501203

Expected Samples:

12 Samples Loaded: 12

Species

Human (Homo sapiens)

Sample Source

Monocytes

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	treatment	cell type	disease state	individual
GSM1127890	monocyte from donor 1 stimulated with CTRL	control	monocyte	healthy	donor 1
GSM1127891	monocyte from donor 2 stimulated with CTRL	control	monocyte	healthy	donor 2
GSM1127892	monocyte from donor 3 stimulated with CTRL	control	monocyte	healthy	donor 3
GSM1127893	monocyte from donor 4 stimulated with CTRL	control	monocyte	healthy	donor 4
GSM1127894	monocyte from donor 1 stimulated with ATRA	all-trans retinoic acid	monocyte	healthy	donor 1

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Sample ID	!Sample Title	Treatment	Cell type	Disease state	Individual
GSM1127890	monocyte from donor 1 stimulated with CTRL	control	monocyte	healthy	donor 1
GSM1127891	monocyte from donor 2 stimulated with CTRL	control	monocyte	healthy	donor 2
GSM1127892	monocyte from donor 3 stimulated with CTRL	control	monocyte	healthy	donor 3
GSM1127893	monocyte from donor 4 stimulated with CTRL	control	monocyte	healthy	donor 4
GSM1127894	monocyte from donor 1 stimulated with ATRA	all-trans retinoic acid	monocyte	healthy	donor 1
GSM1127895	monocyte from donor 2 stimulated with ATRA	all-trans retinoic acid	monocyte	healthy	donor 2
GSM1127896	monocyte from donor 3 stimulated with ATRA	all-trans retinoic acid	monocyte	healthy	donor 3
GSM1127897	monocyte from donor 4 stimulated with ATRA	all-trans retinoic acid	monocyte	healthy	donor 4
GSM1127898	monocyte from donor 1 stimulated with 1,25D	1_25a-dihydroxyvitamin D3	monocyte	healthy	donor 1
GSM1127899	monocyte from donor 2 stimulated with 1,25D	1_25a-dihydroxyvitamin D3	monocyte	healthy	donor 2
GSM1127900	monocyte from donor 3 stimulated with 1,25D	1_25a-dihydroxyvitamin D3	monocyte	healthy	donor 3
GSM1127901	monocyte from donor 4 stimulated with 1,25D	1_25a-dihydroxyvitamin D3	monocyte	healthy	donor 4

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SIDRA