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Clinical

Interaction of bone marrow stroma and monocytes: bone marrow stromal cell lines cultured with monocytes-GSE10595

Purpose

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Hypothesis

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Experimental Design

Two human bone marrow stromal cell lines (HS5 and HS27a) were cultured with and without monocytes (CD14+ cells) from 2 different donors.culture condition: stroma no monocytes: HS5-1, HS5-2, HS27a-1, HS27a-2culture condition: stroma with monocytes: HS5-MO-1, HS5-MO-2, HS27a-MO-1, HS27a-MO-2donor: donor 3: HS5-MO-1, HS27a-MO-1donor: donor 4: HS5-MO-2, HS27a-MO-2cell line: HS5: HS5-1, HS5-2, HS5-MO-1, HS5-MO-2cell line: HS27a: HS27a-1, HS27a-2, HS27a-MO-1, HS27a-MO-2

Experimental Variables

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Controls

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Methods

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Additional Information
  • Iwata M, Sandstrom RS, Delrow JJ, Stamatoyannopoulos JA et al. Functionally and phenotypically distinct subpopulations of marrow stromal cells are fibroblast in origin and induce different fates in peripheral blood monocytes.Stem Cells Dev 2014 Apr 1;23(7):729-40. PMID: 24131213
  • Microarray
    Affymetrix HG-U133_Plus_2
    8 Samples Loaded: 8
    Human (Homo sapiens)
    Bone marrow cell line , Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title Gender Culture conditions NA
    GSM267077 HS5-rep1 HS5 True
    GSM267078 HS5-rep2 HS5 True
    GSM267079 HS5+MO-donor3 M, age: 24 HS5+monocyte
    GSM267080 HS5+MO-donor4 M, age: 31 HS5+monocyte
    GSM267081 HS27a-rep1 HS27a True
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    File
    sampleset4000085_sampleannotations.csv
    Sample Set Spreadsheet
    Raw Signal
    Raw Signal
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    Sample ID !Sample Title Gender Culture conditions NA
    GSM267077
    HS5-rep1
     
    HS5
    True
    GSM267078
    HS5-rep2
     
    HS5
    True
    GSM267079
    HS5+MO-donor3
    M, age: 24
    HS5+monocyte
     
    GSM267080
    HS5+MO-donor4
    M, age: 31
    HS5+monocyte
     
    GSM267081
    HS27a-rep1
     
    HS27a
    True
    GSM267082
    HS27a-rep2
     
    HS27a
    True
    GSM267083
    HS27a+MO-donor3
    M, age: 24
    HS27a+monocyte
     
    GSM267084
    HS27a+MO-donor4
    M, age: 31
    HS27a+monocyte
     

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