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Clinical

Gene expression profile during monocytes to macrophage differentiation-GSE8286

Purpose

To examine the transcriptional profile of in vitro adherence-induced differentiation of primary human monocytes into macrophages.

Hypothesis

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Experimental Design

Total 9 samples were analyzed. Repeats of 3 total RNA samples from monocytes at 0 hour, 3 samples from monocytes at 16 hours, and other 3 samples of monocytes at 168 hours after differentiation were collected. Following the Affymetrix protocol, 5-10 mg of high quality RNA was used to make labeled cRNA. The size distribution of fragmented and unfragmented labeled cRNA were assessed by 2100 Bioanalyzer (Agilent Technologies). 20mg of fragmented labeled cRNA was hybridized to Human U133A GeneChip at 45C for 18 hours. Affymetrix fluidics station was used to perform the washing and staining of the chips. GeneChip 3000 scanner system was used to scan the chips. The data were normalized by dCHIP method.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Liu H, Shi B, Huang CC, Eksarko P et al. Transcriptional diversity during monocyte to macrophage differentiation. Immunol Lett 2008 Apr 15;117(1):70-80. PMID: 18276018
  • Microarray
    Affymetrix HG-U133A
    9 Samples Loaded: 9
    Human (Homo sapiens)
    Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title Normal human blood sample Cuture conditions
    GSM205556 MO7-9, Macropage sample 1 True CD14-168 h
    GSM205558 MOA10-22, Macrophage sample 2 True CD14-168 h
    GSM205585 MOB11-4, Macrophage sample 3 True CD14-168 h
    GSM205587 MONO7-9, Monocytes 0h-sample 1 True CD14-0 h
    GSM205588 MONOB10-22, Monocytes 0h-sample 2 True CD14-0 h
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    File
    sampleset4000084_sampleannotations.csv
    Sample Set Spreadsheet
    Raw Signal
    Raw Signal
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    All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

    Sample ID !Sample Title Normal human blood sample Cuture conditions
    GSM205556
    MO7-9, Macropage sample 1
    True
    CD14-168 h
    GSM205558
    MOA10-22, Macrophage sample 2
    True
    CD14-168 h
    GSM205585
    MOB11-4, Macrophage sample 3
    True
    CD14-168 h
    GSM205587
    MONO7-9, Monocytes 0h-sample 1
    True
    CD14-0 h
    GSM205588
    MONOB10-22, Monocytes 0h-sample 2
    True
    CD14-0 h
    GSM205590
    MONOB11-4, Monocytes 0h-sample 3
    True
    CD14-0 h
    GSM205591
    Mono16h3-31, Monocytes 16h-sample 1
    True
    CD14-16 h
    GSM205592
    Mono16h4-14, Monocytes 16h-sample 2
    True
    CD14-16 h
    GSM205594
    Mono16h4-21, Monocytes 16h-sample 3
    True
    CD14-16 h

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