Sample Set Annotation Tool: Gene expression profile during monocytes to macrophage differentiation-GSE8286

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

To examine the transcriptional profile of in vitro adherence-induced differentiation of primary human monocytes into macrophages.

Hypothesis

Experimental Design

Total 9 samples were analyzed. Repeats of 3 total RNA samples from monocytes at 0 hour, 3 samples from monocytes at 16 hours, and other 3 samples of monocytes at 168 hours after differentiation were collected. Following the Affymetrix protocol, 5-10 mg of high quality RNA was used to make labeled cRNA. The size distribution of fragmented and unfragmented labeled cRNA were assessed by 2100 Bioanalyzer (Agilent Technologies). 20mg of fragmented labeled cRNA was hybridized to Human U133A GeneChip at 45C for 18 hours. Affymetrix fluidics station was used to perform the washing and staining of the chips. GeneChip 3000 scanner system was used to scan the chips. The data were normalized by dCHIP method.

Experimental Variables

Controls

Methods

Additional Information

Liu H, Shi B, Huang CC, Eksarko P et al. Transcriptional diversity during monocyte to macrophage differentiation. Immunol Lett 2008 Apr 15;117(1):70-80. PMID: 18276018

Expected Samples:

9 Samples Loaded: 9

Species

Human (Homo sapiens)

Sample Source

Monocytes

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	Normal human blood sample	Cuture conditions
GSM205556	MO7-9, Macropage sample 1	True	CD14-168 h
GSM205558	MOA10-22, Macrophage sample 2	True	CD14-168 h
GSM205585	MOB11-4, Macrophage sample 3	True	CD14-168 h
GSM205587	MONO7-9, Monocytes 0h-sample 1	True	CD14-0 h
GSM205588	MONOB10-22, Monocytes 0h-sample 2	True	CD14-0 h

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File
sampleset4000084_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Normal human blood sample	Cuture conditions
GSM205556	MO7-9, Macropage sample 1	True	CD14-168 h
GSM205558	MOA10-22, Macrophage sample 2	True	CD14-168 h
GSM205585	MOB11-4, Macrophage sample 3	True	CD14-168 h
GSM205587	MONO7-9, Monocytes 0h-sample 1	True	CD14-0 h
GSM205588	MONOB10-22, Monocytes 0h-sample 2	True	CD14-0 h
GSM205590	MONOB11-4, Monocytes 0h-sample 3	True	CD14-0 h
GSM205591	Mono16h3-31, Monocytes 16h-sample 1	True	CD14-16 h
GSM205592	Mono16h4-14, Monocytes 16h-sample 2	True	CD14-16 h
GSM205594	Mono16h4-21, Monocytes 16h-sample 3	True	CD14-16 h

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