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Altered monocyte expression and expansion of non-classical monocyte subset in IgA nephropathy patients-GSE58539

Purpose

The basic defect of IgA nephropathy (IgAN) lies within peripheral blood mononuclear cells rather than local kidney abnormalities. Previously we showed an altered gene expression in monocytes compared to B and T cells isolated from IgAN patients (Kidney Int, 2010), thus our aim here was to study this subset more closely at genome-wide level.

Hypothesis

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Experimental Design

Total of 35 IgAN patients and 35 healthy blood donors (HBD) were included in this study. Illumina microarray technology was used to evaluate global differences in gene expression between monocytes isolated from IgAN patients and HBD. Bioinformatic analysis was performed with GenomeStudio and Genespring software. The connectivity between genes was evaluated using Ingenuity Pathway Analysis. Aberrantly expressed genes and pathways were then validated on an independent set of patients with RT-PCR western blot and flow cytometric analysis.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Cox SN, Serino G, Sallustio F, Blasi A et al. Altered monocyte expression and expansion of non-classical monocyte subset in IgA nephropathy patients.Nephrol Dial Transplant 2015 Jul;30(7):1122-232. PMID: 25770168
  • Microarray
    Illumina HumanHT-12 v4
    17 Samples Loaded: 17
    Human (Homo sapiens)
    Monocytes
    Nephropathy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title cell type disease status
    GSM1413151 5786057055_A peripheral blood mononuclear cells (PBMCs) Affected IgAN
    GSM1413152 5786057055_B peripheral blood mononuclear cells (PBMCs) Affected IgAN
    GSM1413153 5786057055_C peripheral blood mononuclear cells (PBMCs) Affected IgAN
    GSM1413154 5786057055_D peripheral blood mononuclear cells (PBMCs) Affected IgAN
    GSM1413155 5786057055_E peripheral blood mononuclear cells (PBMCs) Affected IgAN
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    All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

    Sample ID !Sample Title Cell type Disease status
    GSM1413151
    5786057055_A
    peripheral blood mononuclear cells (PBMCs)
    Affected IgAN
    GSM1413152
    5786057055_B
    peripheral blood mononuclear cells (PBMCs)
    Affected IgAN
    GSM1413153
    5786057055_C
    peripheral blood mononuclear cells (PBMCs)
    Affected IgAN
    GSM1413154
    5786057055_D
    peripheral blood mononuclear cells (PBMCs)
    Affected IgAN
    GSM1413155
    5786057055_E
    peripheral blood mononuclear cells (PBMCs)
    Affected IgAN
    GSM1413156
    5786057055_F
    peripheral blood mononuclear cells (PBMCs)
    Affected IgAN
    GSM1413157
    5786057055_J
    peripheral blood mononuclear cells (PBMCs)
    Affected IgAN
    GSM1413158
    5786057055_K
    peripheral blood mononuclear cells (PBMCs)
    Affected IgAN
    GSM1413159
    5786057055_G
    peripheral blood mononuclear cells (PBMCs)
    Healthy Blood Donor
    GSM1413160
    5786057055_H
    peripheral blood mononuclear cells (PBMCs)
    Healthy Blood Donor
    GSM1413161
    5786057055_I
    peripheral blood mononuclear cells (PBMCs)
    Healthy Blood Donor
    GSM1413162
    5786057055_L
    peripheral blood mononuclear cells (PBMCs)
    Healthy Blood Donor
    GSM1413163
    5786057034_B
    peripheral blood mononuclear cells (PBMCs)
    Healthy Blood Donor
    GSM1413164
    5786057034_C
    peripheral blood mononuclear cells (PBMCs)
    Healthy Blood Donor
    GSM1413165
    5786057034_E
    peripheral blood mononuclear cells (PBMCs)
    Healthy Blood Donor
    GSM1413166
    5786057034_G
    peripheral blood mononuclear cells (PBMCs)
    Healthy Blood Donor
    GSM1413167
    5786057034_I
    peripheral blood mononuclear cells (PBMCs)
    Healthy Blood Donor

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