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Clinical

Gene expression profiles of human blood classical monocytes (CD14++CD16-), CD16 positive monocytes (CD14+16++ and CD14++CD16+), and CD1c+ CD19- dendritic cells-GSE34515

Purpose

Gene expression of human blood classical monocytes (CD14++CD16-), CD16 positive monocytes (consisting of non-classical CD14+16++ and intermediate CD14++CD16+ monocytes) and CD1c+ CD19- dendritic cells from healthy subjects were investigated.Keywords: expression profiling by array

Hypothesis

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Experimental Design

The two major monocyte subsets and the CD1+ blood dendritic cells (DCs CD1c+ CD19-) were isolated from venous heparinized blood of three apparently healthy human volunteers (donors 1, 2 and 3) using MACS technology with all reagents and tools from Miltenyi Biotec. RNA was isolated and 1 µg was labeled using the Affymetrix One-Cycle Target Labeling Kit and the biotinylated cRNA used to perform Affymetrix U133 Plus 2.0 expression arrays. The data sets for CD14++CD16- classical monocytes are tagged CD16neg, the data sets for CD16 positive monocytes are tagged CD16pos and the data sets for the CD1c+CD19- DCs are tagged CD1cpos.

Experimental Variables

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Controls

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Methods

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Additional Information

Ziegler-Heitbrock L, Prazeres da Costa O

Microarray
Affymetrix HG-U133_Plus_2
9 Samples Loaded: 9
Human (Homo sapiens)
Dendritic Cells , Monocytes
Healthy
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample_title tissue cell subtype
GSM850440 CD16neg1 blood dendritic cells CD16neg
GSM850441 CD16neg2 blood dendritic cells CD16neg
GSM850442 CD16neg3 blood dendritic cells CD16neg
GSM850443 CD16pos1 blood dendritic cells CD16pos
GSM850444 CD16pos2 blood dendritic cells CD16pos
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Log2 Transformed
Raw Signal
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Sample ID !Sample Title Tissue Cell subtype
GSM850440
CD16neg1
blood dendritic cells
CD16neg
GSM850441
CD16neg2
blood dendritic cells
CD16neg
GSM850442
CD16neg3
blood dendritic cells
CD16neg
GSM850443
CD16pos1
blood dendritic cells
CD16pos
GSM850444
CD16pos2
blood dendritic cells
CD16pos
GSM850445
CD16pos3
blood dendritic cells
CD16pos
GSM850446
CD1cpos1
blood dendritic cells
CD1c+
GSM850447
CD1cpos2
blood dendritic cells
CD1c+
GSM850448
CD1cpos3
blood dendritic cells
CD1c+

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