Gene expression profiles of human blood classical monocytes (CD14++CD16-), CD16 positive monocytes (CD14+16++ and CD14++CD16+), and CD1c+ CD19- dendritic cells-GSE34515
Gene expression of human blood classical monocytes (CD14++CD16-), CD16 positive monocytes (consisting of non-classical CD14+16++ and intermediate CD14++CD16+ monocytes) and CD1c+ CD19- dendritic cells from healthy subjects were investigated.Keywords: expression profiling by array
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The two major monocyte subsets and the CD1+ blood dendritic cells (DCs CD1c+ CD19-) were isolated from venous heparinized blood of three apparently healthy human volunteers (donors 1, 2 and 3) using MACS technology with all reagents and tools from Miltenyi Biotec. RNA was isolated and 1 µg was labeled using the Affymetrix One-Cycle Target Labeling Kit and the biotinylated cRNA used to perform Affymetrix U133 Plus 2.0 expression arrays. The data sets for CD14++CD16- classical monocytes are tagged CD16neg, the data sets for CD16 positive monocytes are tagged CD16pos and the data sets for the CD1c+CD19- DCs are tagged CD1cpos.
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Ziegler-Heitbrock L, Prazeres da Costa O
Sample ID | !Sample_title | tissue | cell subtype |
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GSM850440 | CD16neg1 | blood dendritic cells | CD16neg |
GSM850441 | CD16neg2 | blood dendritic cells | CD16neg |
GSM850442 | CD16neg3 | blood dendritic cells | CD16neg |
GSM850443 | CD16pos1 | blood dendritic cells | CD16pos |
GSM850444 | CD16pos2 | blood dendritic cells | CD16pos |
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Sample ID | !Sample Title | Tissue | Cell subtype |
---|---|---|---|
GSM850440 | CD16neg1 |
blood dendritic cells |
CD16neg |
GSM850441 | CD16neg2 |
blood dendritic cells |
CD16neg |
GSM850442 | CD16neg3 |
blood dendritic cells |
CD16neg |
GSM850443 | CD16pos1 |
blood dendritic cells |
CD16pos |
GSM850444 | CD16pos2 |
blood dendritic cells |
CD16pos |
GSM850445 | CD16pos3 |
blood dendritic cells |
CD16pos |
GSM850446 | CD1cpos1 |
blood dendritic cells |
CD1c+ |
GSM850447 | CD1cpos2 |
blood dendritic cells |
CD1c+ |
GSM850448 | CD1cpos3 |
blood dendritic cells |
CD1c+ |
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