Gene expression profiling of the classical (CD14++CD16-), intermediate (CD14++CD16+) and nonclassical (CD14+CD16+) human monocyte subsets-GSE25913
The new official nomenclature subdivides human monocytes into three subsets, classical (CD14++CD16-), intermediate (CD14++CD16+) and nonclassical (CD14+CD16+). Here, we comprehensively define relationships and unique characteristics of the three human monocyte subsets using microarray and flow cytometry analysis
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Three human monocyte subsets, the CD14++CD16- classical, the CD14++CD16+ intermediate and CD14+CD16+ nonclassical subsets were purified using fluorescence activated cell sorting from peripheral blood mononuclear cells. RNA was processed from the three monocyte subsets from 4 individual donors in duplicates, giving a total of 24 samples.
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Sample ID | !Sample_title | donor | cell type | monocyte subset | rep |
---|---|---|---|---|---|
GSM636767 | Healthy_blood_CD14++CD16-_monocyte_donor1_rep1 | 1 | monocyte | Classical | 1 |
GSM636768 | Healthy_blood_CD14++CD16+_monocytes_donor1_rep1 | 1 | monocyte | Intermediate | 1 |
GSM636769 | Healthy_blood_CD14+CD16+_monocytes_donor1_rep1 | 1 | monocyte | Nonclassical | 1 |
GSM636770 | Healthy_blood_CD14++CD16-_monocyte_donor2_rep1 | 2 | monocyte | Classical | 1 |
GSM636771 | Healthy_blood_CD14++CD16+_monocytes_donor2_rep1 | 2 | monocyte | Intermediate | 1 |
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Sample ID | !Sample Title | Donor | Cell type | Monocyte subset | Rep |
---|---|---|---|---|---|
GSM636767 | Healthy_blood_CD14++CD16-_monocyte_donor1_rep1 |
1 |
monocyte |
Classical |
1 |
GSM636768 | Healthy_blood_CD14++CD16+_monocytes_donor1_rep1 |
1 |
monocyte |
Intermediate |
1 |
GSM636769 | Healthy_blood_CD14+CD16+_monocytes_donor1_rep1 |
1 |
monocyte |
Nonclassical |
1 |
GSM636770 | Healthy_blood_CD14++CD16-_monocyte_donor2_rep1 |
2 |
monocyte |
Classical |
1 |
GSM636771 | Healthy_blood_CD14++CD16+_monocytes_donor2_rep1 |
2 |
monocyte |
Intermediate |
1 |
GSM636772 | Healthy_blood_CD14+CD16+_monocytes_donor2_rep1 |
2 |
monocyte |
Nonclassical |
1 |
GSM636773 | Healthy_blood_CD14++CD16-_monocyte_donor3_rep1 |
3 |
monocyte |
Classical |
1 |
GSM636774 | Healthy_blood_CD14++CD16+_monocytes_donor3_rep1 |
3 |
monocyte |
Intermediate |
1 |
GSM636775 | Healthy_blood_CD14+CD16+_monocytes_donor3_rep1 |
3 |
monocyte |
Nonclassical |
1 |
GSM636776 | Healthy_blood_CD14++CD16-_monocyte_donor4_rep1 |
4 |
monocyte |
Classical |
1 |
GSM636777 | Healthy_blood_CD14++CD16+_monocytes_donor4_rep1 |
4 |
monocyte |
Intermediate |
1 |
GSM636778 | Healthy_blood_CD14+CD16+_monocytes_donor4_rep1 |
4 |
monocyte |
Nonclassical |
1 |
GSM636779 | Healthy_blood_CD14++CD16-_monocyte_donor1_rep2 |
1 |
monocyte |
Classical |
2 |
GSM636780 | Healthy_blood_CD14++CD16+_monocytes_donor1_rep2 |
1 |
monocyte |
Intermediate |
2 |
GSM636781 | Healthy_blood_CD14+CD16+_monocytes_donor1_rep2 |
1 |
monocyte |
Nonclassical |
2 |
GSM636782 | Healthy_blood_CD14++CD16-_monocyte_donor2_rep2 |
2 |
monocyte |
Classical |
2 |
GSM636783 | Healthy_blood_CD14++CD16+_monocytes_donor2_rep2 |
2 |
monocyte |
Intermediate |
2 |
GSM636784 | Healthy_blood_CD14+CD16+_monocytes_donor2_rep2 |
2 |
monocyte |
Nonclassical |
2 |
GSM636785 | Healthy_blood_CD14++CD16-_monocyte_donor3_rep2 |
3 |
monocyte |
Classical |
2 |
GSM636786 | Healthy_blood_CD14++CD16+_monocytes_donor3_rep2 |
3 |
monocyte |
Intermediate |
2 |
GSM636787 | Healthy_blood_CD14+CD16+_monocytes_donor3_rep2 |
3 |
monocyte |
Nonclassical |
2 |
GSM636788 | Healthy_blood_CD14++CD16-_monocyte_donor4_rep2 |
4 |
monocyte |
Classical |
2 |
GSM636789 | Healthy_blood_CD14++CD16+_monocytes_donor4_rep2 |
4 |
monocyte |
Intermediate |
2 |
GSM636790 | Healthy_blood_CD14+CD16+_monocytes_donor4_rep2 |
4 |
monocyte |
Nonclassical |
2 |
Name | ||
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