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Clinical

Gene expression profiling of the classical (CD14++CD16-), intermediate (CD14++CD16+) and nonclassical (CD14+CD16+) human monocyte subsets-GSE25913

Purpose

The new official nomenclature subdivides human monocytes into three subsets, classical (CD14++CD16-), intermediate (CD14++CD16+) and nonclassical (CD14+CD16+). Here, we comprehensively define relationships and unique characteristics of the three human monocyte subsets using microarray and flow cytometry analysis

Hypothesis

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Experimental Design

Three human monocyte subsets, the CD14++CD16- classical, the CD14++CD16+ intermediate and CD14+CD16+ nonclassical subsets were purified using fluorescence activated cell sorting from peripheral blood mononuclear cells. RNA was processed from the three monocyte subsets from 4 individual donors in duplicates, giving a total of 24 samples.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Wong KL, Tai JJ, Wong WC, Han H et al. Gene expression profiling reveals the defining features of the classical, intermediate, and nonclassical human monocyte subsets. Blood 2011 Aug 4;118(5):e16-31. PMID: 21653326
  • Microarray
    Illumina HumanWG-6 v2
    24 Samples Loaded: 24
    Human (Homo sapiens)
    Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title donor cell type monocyte subset rep
    GSM636767 Healthy_blood_CD14++CD16-_monocyte_donor1_rep1 1 monocyte Classical 1
    GSM636768 Healthy_blood_CD14++CD16+_monocytes_donor1_rep1 1 monocyte Intermediate 1
    GSM636769 Healthy_blood_CD14+CD16+_monocytes_donor1_rep1 1 monocyte Nonclassical 1
    GSM636770 Healthy_blood_CD14++CD16-_monocyte_donor2_rep1 2 monocyte Classical 1
    GSM636771 Healthy_blood_CD14++CD16+_monocytes_donor2_rep1 2 monocyte Intermediate 1
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    Raw Signal
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    Sample ID !Sample Title Donor Cell type Monocyte subset Rep
    GSM636767
    Healthy_blood_CD14++CD16-_monocyte_donor1_rep1
    1
    monocyte
    Classical
    1
    GSM636768
    Healthy_blood_CD14++CD16+_monocytes_donor1_rep1
    1
    monocyte
    Intermediate
    1
    GSM636769
    Healthy_blood_CD14+CD16+_monocytes_donor1_rep1
    1
    monocyte
    Nonclassical
    1
    GSM636770
    Healthy_blood_CD14++CD16-_monocyte_donor2_rep1
    2
    monocyte
    Classical
    1
    GSM636771
    Healthy_blood_CD14++CD16+_monocytes_donor2_rep1
    2
    monocyte
    Intermediate
    1
    GSM636772
    Healthy_blood_CD14+CD16+_monocytes_donor2_rep1
    2
    monocyte
    Nonclassical
    1
    GSM636773
    Healthy_blood_CD14++CD16-_monocyte_donor3_rep1
    3
    monocyte
    Classical
    1
    GSM636774
    Healthy_blood_CD14++CD16+_monocytes_donor3_rep1
    3
    monocyte
    Intermediate
    1
    GSM636775
    Healthy_blood_CD14+CD16+_monocytes_donor3_rep1
    3
    monocyte
    Nonclassical
    1
    GSM636776
    Healthy_blood_CD14++CD16-_monocyte_donor4_rep1
    4
    monocyte
    Classical
    1
    GSM636777
    Healthy_blood_CD14++CD16+_monocytes_donor4_rep1
    4
    monocyte
    Intermediate
    1
    GSM636778
    Healthy_blood_CD14+CD16+_monocytes_donor4_rep1
    4
    monocyte
    Nonclassical
    1
    GSM636779
    Healthy_blood_CD14++CD16-_monocyte_donor1_rep2
    1
    monocyte
    Classical
    2
    GSM636780
    Healthy_blood_CD14++CD16+_monocytes_donor1_rep2
    1
    monocyte
    Intermediate
    2
    GSM636781
    Healthy_blood_CD14+CD16+_monocytes_donor1_rep2
    1
    monocyte
    Nonclassical
    2
    GSM636782
    Healthy_blood_CD14++CD16-_monocyte_donor2_rep2
    2
    monocyte
    Classical
    2
    GSM636783
    Healthy_blood_CD14++CD16+_monocytes_donor2_rep2
    2
    monocyte
    Intermediate
    2
    GSM636784
    Healthy_blood_CD14+CD16+_monocytes_donor2_rep2
    2
    monocyte
    Nonclassical
    2
    GSM636785
    Healthy_blood_CD14++CD16-_monocyte_donor3_rep2
    3
    monocyte
    Classical
    2
    GSM636786
    Healthy_blood_CD14++CD16+_monocytes_donor3_rep2
    3
    monocyte
    Intermediate
    2
    GSM636787
    Healthy_blood_CD14+CD16+_monocytes_donor3_rep2
    3
    monocyte
    Nonclassical
    2
    GSM636788
    Healthy_blood_CD14++CD16-_monocyte_donor4_rep2
    4
    monocyte
    Classical
    2
    GSM636789
    Healthy_blood_CD14++CD16+_monocytes_donor4_rep2
    4
    monocyte
    Intermediate
    2
    GSM636790
    Healthy_blood_CD14+CD16+_monocytes_donor4_rep2
    4
    monocyte
    Nonclassical
    2

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