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Clinical

Filaria induced monocyte dysfunction and its reversal following treatment-GSE2135

Purpose

To assess the function of peripheral blood derived monocytes in patently infected filaria patients (either Wuchereria bancrofti, Mansonella perstans, or both).

Hypothesis

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Experimental Design

We investigated the cytokine production and gene expression profile of these cells in filaria infected patients and compared them with those from uninfected normal blood bank donors. Monocytes from infected individuals were studded with intracellular microfilarial antigens. In addition, Staphylococcus aureus Cowan I bacteria (SAC)/IFN ? activated monocytes from the infected individuals produced less IL 8, Exodus II, MIP 1a, MIP 1b, eotaxin, RANTES, IL 1a, and MIP 3b compared with normal patients. Microarray analysis of ex vivo isolated monocytes demonstrated that multiple genes involved in signal transduction, apoptosis, and adhesion were expressed to a greater degree in filaria infected patient monocytes compared with those of uninfected normal individuals. Eight months following treatment with a single dose of ivermectin/albendazole, monocytes of W. bancrofti infected individuals were capable of producing more IL 8, IL 1a, MIP 1a, and IL 10 in response to SAC/IFN ? compared with their pretreatment levels. Further, when monocyte global expression was compared before and after treatment of the W. bancrofti, there was a marked increase in mRNA expression of genes associated with protein metabolism, and in HSP most particularly, compared with pretreatment expression.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Semnani RT, Keiser PB, Coulibaly YI, Keita F et al. Filaria-induced monocyte dysfunction and its reversal following treatment. Infect Immun 2006 Aug;74(8):4409-17. PMID: 16861626
  • Microarray
    Affymetrix HG-U133A
    14 Samples Loaded: 14
    Human (Homo sapiens)
    Monocytes
    Filariasis
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title Sample conditions
    GSM38551 M02-006 post-treatment Post-Treat
    GSM38552 M02-006 pre-treatment Pre-Treat
    GSM38553 M02-039 post-treatment Post-Treat
    GSM38554 M02-039 pretreatment Pre-Treat
    GSM38555 M02-133 post-treatment Post-Treat
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    sampleset4000068_sampleannotations.csv
    Sample Set Spreadsheet
    Raw Signal
    Raw Signal
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    Sample ID !Sample Title Sample conditions
    GSM38551
    M02-006 post-treatment
    Post-Treat
    GSM38552
    M02-006 pre-treatment
    Pre-Treat
    GSM38553
    M02-039 post-treatment
    Post-Treat
    GSM38554
    M02-039 pretreatment
    Pre-Treat
    GSM38555
    M02-133 post-treatment
    Post-Treat
    GSM38556
    M02-133 pre-treatment
    Pre-Treat
    GSM38558
    M02-097 post-treatment
    Post-Treat
    GSM38559
    M02-097 pre-treatment
    Pre-Treat
    GSM38560
    M02-110 post-treatment
    Post-Treat
    GSM38561
    M02-110 pre-treatment
    Pre-Treat
    GSM38562
    M02-147 post-treatment
    Post-Treat
    GSM38563
    M02-147 pre-treatment
    Pre-Treat
    GSM38565
    NORMAL 1
    Control
    GSM38566
    NORMAL 2
    Control

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