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Clinical

Mycobacterium tuberculosis Chaperonin 60.1 has Bipolar Effects on Human peripheral blood-derived Monocytes-GSE18794

Purpose

To study the pro-inflammatory activities of Cpn60.1 in human monocytes

Hypothesis

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Experimental Design

Human monocytes were purified from three healthy donors and incubated with 10 ?g/ml M. tuberculosis Cpn60.1 (or 100 ng/ml LPS as a positive control or medium only as a negative control). To study the inhibitory effectds of Cpn60.1 on human monocytes, the cells were pre-exposed to 10?g/ml or 0.1ng/ml of Cpn60.1, washed and incubated with 1ng/ml LPS for further 2 hours. For positive control, the cells were pre-exposed to medium before washing and LPS stimulation while for the negative control, the cells were pre-exposed to medium, washed and again exposed to medium alone.

Experimental Variables

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Controls

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Methods

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Additional Information

Cehovin A, Henderson B, Coates AR, Waddell SJ

Microarray
Affymetrix HuGene 1.0 ST v1
21 Samples Loaded: 21
Human (Homo sapiens)
Monocytes
Healthy
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample_title cell type agent Culture conditions
GSM466274 HuMonocytes_LPS_2hr_rep1 Human peripheral blood-derived monocytes incubated with 100ng/ml LPS for 2 hrs LPS 2h
GSM466275 HuMonocytes_LPS_2hr_rep2 Human peripheral blood-derived monocytes incubated with 100ng/ml LPS for 2 hrs LPS 2h
GSM466276 HuMonocytes_LPS_2hr_rep3 Human peripheral blood-derived monocytes incubated with 100ng/ml LPS for 2 hrs LPS 2h
GSM466277 HuMonocytes_Medium_2hr_rep1 Human peripheral blood-derived monocytes incubated in medium alone for 2 hrs Medium 2h
GSM466278 HuMonocytes_Medium_2hr_rep2 Human peripheral blood-derived monocytes incubated in medium alone for 2 hrs Medium 2h
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File
sampleset4000067_sampleannotations.csv
Sample Set Spreadsheet
Log2 Transformed
Raw Signal
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Sample ID !Sample Title Cell type Agent Culture conditions
GSM466274
HuMonocytes_LPS_2hr_rep1
Human peripheral blood-derived monocytes
incubated with 100ng/ml LPS for 2 hrs
LPS 2h
GSM466275
HuMonocytes_LPS_2hr_rep2
Human peripheral blood-derived monocytes
incubated with 100ng/ml LPS for 2 hrs
LPS 2h
GSM466276
HuMonocytes_LPS_2hr_rep3
Human peripheral blood-derived monocytes
incubated with 100ng/ml LPS for 2 hrs
LPS 2h
GSM466277
HuMonocytes_Medium_2hr_rep1
Human peripheral blood-derived monocytes
incubated in medium alone for 2 hrs
Medium 2h
GSM466278
HuMonocytes_Medium_2hr_rep2
Human peripheral blood-derived monocytes
incubated in medium alone for 2 hrs
Medium 2h
GSM466279
HuMonocytes_Medium_2hr_rep3
Human peripheral blood-derived monocytes
incubated in medium alone for 2 hrs
Medium 2h
GSM466280
HuMonocytes_Cpn60.1_2hr_rep1
Human peripheral blood-derived monocytes
incubated with 10μg/ml Cpn60.1 for 2 hrs
CPN60.1 2h
GSM466281
HuMonocytes_Cpn60.1_2hr_rep2
Human peripheral blood-derived monocytes
incubated with 10μg/ml Cpn60.1 for 2 hrs
CPN60.1 2h
GSM466282
HuMonocytes_Cpn60.1_2hr_rep3
Human peripheral blood-derived monocytes
incubated with 10μg/ml Cpn60.1 for 2 hrs
CPN60.1 2h
GSM466283
HuMonocytes_Medium_1hr_LPS_2hr_rep1
Human peripheral blood-derived monocytes
pre-incubated in medium for 1 hr, stimulated with 1ng/ml LPS for 2 hrs
Pre-Medium+LPS 2h
GSM466284
HuMonocytes_Medium_1hr_LPS_2hr_rep2
Human peripheral blood-derived monocytes
pre-incubated in medium for 1 hr, stimulated with 1ng/ml LPS for 2 hrs
Pre-Medium+LPS 2h
GSM466285
HuMonocytes_Medium_1hr_LPS_2hr_rep3
Human peripheral blood-derived monocytes
pre-incubated in medium for 1 hr, stimulated with 1ng/ml LPS for 2 hrs
Pre-Medium+LPS 2h
GSM466286
HuMonocytes_Medium_1hr_Medium_2hr_rep1
Human peripheral blood-derived monocytes
pre-incubated in medium for 1 hr, incubated in medium for 2 hrs
Pre-Medium+Medium 2h
GSM466287
HuMonocytes_Medium_1hr_Medium_2hr_rep2
Human peripheral blood-derived monocytes
pre-incubated in medium for 1 hr, incubated in medium for 2 hrs
Pre-Medium+Medium 2h
GSM466288
HuMonocytes_Medium_1hr_Medium_2hr_rep3
Human peripheral blood-derived monocytes
pre-incubated in medium for 1 hr, incubated in medium for 2 hrs
Pre-Medium+Medium 2h
GSM466289
HuMonocytes_Cpn60.1 10_1hr_LPS_2hr_rep1
Human peripheral blood-derived monocytes
pre-incubated with 10μg/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs
Pre-CPN60.1+LPS 2h
GSM466290
HuMonocytes_Cpn60.1 10_1hr_LPS_2hr_rep2
Human peripheral blood-derived monocytes
pre-incubated with 10μg/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs
Pre-CPN60.1+LPS 2h
GSM466291
HuMonocytes_Cpn60.1 10_1hr_LPS_2hr_rep3
Human peripheral blood-derived monocytes
pre-incubated with 10μg/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs
Pre-CPN60.1+LPS 2h
GSM466292
HuMonocytes_Cpn60.1 0.1_1hr_LPS_2hr_rep1
Human peripheral blood-derived monocytes
pre-incubated with 0.1ng/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs
Pre-CPN60.1+LPS 2h
GSM466293
HuMonocytes_Cpn60.1 0.1_1hr_LPS_2hr_rep2
Human peripheral blood-derived monocytes
pre-incubated with 0.1ng/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs
Pre-CPN60.1+LPS 2h
GSM466294
HuMonocytes_Cpn60.1 0.1_1hr_LPS_2hr_rep3
Human peripheral blood-derived monocytes
pre-incubated with 0.1ng/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs
Pre-CPN60.1+LPS 2h

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