Sample Set Annotation Tool: Mycobacterium tuberculosis Chaperonin 60.1 has Bipolar Effects on Human peripheral blood-derived Monocytes-GSE18794

Study Design
Assay
Sample Info
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Analysis

Purpose

To study the pro-inflammatory activities of Cpn60.1 in human monocytes

Hypothesis

Experimental Design

Human monocytes were purified from three healthy donors and incubated with 10 ?g/ml M. tuberculosis Cpn60.1 (or 100 ng/ml LPS as a positive control or medium only as a negative control). To study the inhibitory effectds of Cpn60.1 on human monocytes, the cells were pre-exposed to 10?g/ml or 0.1ng/ml of Cpn60.1, washed and incubated with 1ng/ml LPS for further 2 hours. For positive control, the cells were pre-exposed to medium before washing and LPS stimulation while for the negative control, the cells were pre-exposed to medium, washed and again exposed to medium alone.

Experimental Variables

Controls

Methods

Additional Information

Cehovin A, Henderson B, Coates AR, Waddell SJ

Expected Samples:

21 Samples Loaded: 21

Species

Human (Homo sapiens)

Sample Source

Monocytes

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Sample ID	!Sample_title	cell type	agent	Culture conditions
GSM466274	HuMonocytes_LPS_2hr_rep1	Human peripheral blood-derived monocytes	incubated with 100ng/ml LPS for 2 hrs	LPS 2h
GSM466275	HuMonocytes_LPS_2hr_rep2	Human peripheral blood-derived monocytes	incubated with 100ng/ml LPS for 2 hrs	LPS 2h
GSM466276	HuMonocytes_LPS_2hr_rep3	Human peripheral blood-derived monocytes	incubated with 100ng/ml LPS for 2 hrs	LPS 2h
GSM466277	HuMonocytes_Medium_2hr_rep1	Human peripheral blood-derived monocytes	incubated in medium alone for 2 hrs	Medium 2h
GSM466278	HuMonocytes_Medium_2hr_rep2	Human peripheral blood-derived monocytes	incubated in medium alone for 2 hrs	Medium 2h

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File
sampleset4000067_sampleannotations.csv Sample Set Spreadsheet

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Sample ID	!Sample Title	Cell type	Agent	Culture conditions
GSM466274	HuMonocytes_LPS_2hr_rep1	Human peripheral blood-derived monocytes	incubated with 100ng/ml LPS for 2 hrs	LPS 2h
GSM466275	HuMonocytes_LPS_2hr_rep2	Human peripheral blood-derived monocytes	incubated with 100ng/ml LPS for 2 hrs	LPS 2h
GSM466276	HuMonocytes_LPS_2hr_rep3	Human peripheral blood-derived monocytes	incubated with 100ng/ml LPS for 2 hrs	LPS 2h
GSM466277	HuMonocytes_Medium_2hr_rep1	Human peripheral blood-derived monocytes	incubated in medium alone for 2 hrs	Medium 2h
GSM466278	HuMonocytes_Medium_2hr_rep2	Human peripheral blood-derived monocytes	incubated in medium alone for 2 hrs	Medium 2h
GSM466279	HuMonocytes_Medium_2hr_rep3	Human peripheral blood-derived monocytes	incubated in medium alone for 2 hrs	Medium 2h
GSM466280	HuMonocytes_Cpn60.1_2hr_rep1	Human peripheral blood-derived monocytes	incubated with 10μg/ml Cpn60.1 for 2 hrs	CPN60.1 2h
GSM466281	HuMonocytes_Cpn60.1_2hr_rep2	Human peripheral blood-derived monocytes	incubated with 10μg/ml Cpn60.1 for 2 hrs	CPN60.1 2h
GSM466282	HuMonocytes_Cpn60.1_2hr_rep3	Human peripheral blood-derived monocytes	incubated with 10μg/ml Cpn60.1 for 2 hrs	CPN60.1 2h
GSM466283	HuMonocytes_Medium_1hr_LPS_2hr_rep1	Human peripheral blood-derived monocytes	pre-incubated in medium for 1 hr, stimulated with 1ng/ml LPS for 2 hrs	Pre-Medium+LPS 2h
GSM466284	HuMonocytes_Medium_1hr_LPS_2hr_rep2	Human peripheral blood-derived monocytes	pre-incubated in medium for 1 hr, stimulated with 1ng/ml LPS for 2 hrs	Pre-Medium+LPS 2h
GSM466285	HuMonocytes_Medium_1hr_LPS_2hr_rep3	Human peripheral blood-derived monocytes	pre-incubated in medium for 1 hr, stimulated with 1ng/ml LPS for 2 hrs	Pre-Medium+LPS 2h
GSM466286	HuMonocytes_Medium_1hr_Medium_2hr_rep1	Human peripheral blood-derived monocytes	pre-incubated in medium for 1 hr, incubated in medium for 2 hrs	Pre-Medium+Medium 2h
GSM466287	HuMonocytes_Medium_1hr_Medium_2hr_rep2	Human peripheral blood-derived monocytes	pre-incubated in medium for 1 hr, incubated in medium for 2 hrs	Pre-Medium+Medium 2h
GSM466288	HuMonocytes_Medium_1hr_Medium_2hr_rep3	Human peripheral blood-derived monocytes	pre-incubated in medium for 1 hr, incubated in medium for 2 hrs	Pre-Medium+Medium 2h
GSM466289	HuMonocytes_Cpn60.1 10_1hr_LPS_2hr_rep1	Human peripheral blood-derived monocytes	pre-incubated with 10μg/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs	Pre-CPN60.1+LPS 2h
GSM466290	HuMonocytes_Cpn60.1 10_1hr_LPS_2hr_rep2	Human peripheral blood-derived monocytes	pre-incubated with 10μg/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs	Pre-CPN60.1+LPS 2h
GSM466291	HuMonocytes_Cpn60.1 10_1hr_LPS_2hr_rep3	Human peripheral blood-derived monocytes	pre-incubated with 10μg/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs	Pre-CPN60.1+LPS 2h
GSM466292	HuMonocytes_Cpn60.1 0.1_1hr_LPS_2hr_rep1	Human peripheral blood-derived monocytes	pre-incubated with 0.1ng/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs	Pre-CPN60.1+LPS 2h
GSM466293	HuMonocytes_Cpn60.1 0.1_1hr_LPS_2hr_rep2	Human peripheral blood-derived monocytes	pre-incubated with 0.1ng/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs	Pre-CPN60.1+LPS 2h
GSM466294	HuMonocytes_Cpn60.1 0.1_1hr_LPS_2hr_rep3	Human peripheral blood-derived monocytes	pre-incubated with 0.1ng/ml Cpn60.1 for 1 hr, stimulated with 1ng/ml LPS for 2 hrs	Pre-CPN60.1+LPS 2h

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