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Clinical

Comparison of gene expression profiles between human and mouse monocyte subsets [human data]-GSE18565

Purpose

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Hypothesis

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Experimental Design

The two major subsets of monocytes (Ly-6C+ and Ly-6Clo) from 12-week old C57Bl/6 mice were sorted and the RNA extracted and hybridized to Affymetrix GeneChip® 430 2.0 arrays. We pooled leukocytes from 5 mice for each sort and sorted 4 separate times for 4 biological replicates. The two major monocyte subsets (CD16- and CD16+) were isolated from venous heparinized blood from apparently healthy human volunteers using MACS technology with all reagents and tools from Miltenyi Biotec. Three separate donors were hybridized three different times to Affymetrix U133 Plus 2.0 array.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Ingersoll MA, Spanbroek R, Lottaz C, Gautier EL et al. Comparison of gene expression profiles between human and mouse monocyte subsets. Blood 2010 Jan 21;115(3):e10-9. PMID: 19965649
  • Microarray
    Affymetrix HG-U133_Plus_2
    6 Samples Loaded: 6
    Human (Homo sapiens)
    Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title monocyte subsets
    GSM432175 CD16- 1 CD16- monocytes
    GSM432176 CD16- 2 CD16- monocytes
    GSM432177 CD16- 3 CD16- monocytes
    GSM432178 CD16+ 1 CD16+ monocytes
    GSM432179 CD16+ 2 CD16+ monocytes
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    Raw Signal
    Raw Signal
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    Sample ID !Sample Title Monocyte subsets
    GSM432175
    CD16- 1
    CD16- monocytes
    GSM432176
    CD16- 2
    CD16- monocytes
    GSM432177
    CD16- 3
    CD16- monocytes
    GSM432178
    CD16+ 1
    CD16+ monocytes
    GSM432179
    CD16+ 2
    CD16+ monocytes
    GSM432180
    CD16+ 3
    CD16+ monocytes

    Group Sets View in Gene Expression Browser

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    Monocyte subsets
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