You are not logged in. | Log in
In Progress
Clinical

Transcriptional profiling of CD16+ and CD16- peripheral blood monocytes from healthy individuals-GSE16836

Purpose

To gain insight into the developmental relationship and functions of CD16+ and CD16- Monocytes

Hypothesis

--

Experimental Design

Total Mo were isolated by negative selection using magnetic immunobeads (Monocyte Isolation Kit II, Miltenyi). CD16+ and CD16- Mo fractions were further isolated using CD16 magnetic immunobeads (Miltenyi). Total RNA from Mo pellets was isolated by Trizol extraction and purified using RNeasy columns (Qiagen). The quality of RNA was assessed by visualization of intact bands corresponding to 18S and 28S rRNA on formaldehyde agarose gels. Total RNA (10 µg) from matched CD16+ and CD16- Mo samples isolated from 4 different healthy donors was quality tested using an Agilent 2100 Bioanalyzer chip, reverse transcribed, and hybridized on the GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix), which includes 54,000 probe sets on a single array (i.e., 47,000 transcripts and variants, including 38,500 well-characterized human genes). Primary data analysis performed using GeneSpring software (Biopolymer core facility, Harvard Medical School) generated Excel spreadsheets with relative gene expression values for the 4 matched CD16+ and CD16- Mo subsets.

Experimental Variables

--

Controls

--

Methods

--

Additional Information
  • Ancuta P, Liu KY, Misra V, Wacleche VS et al. Transcriptional profiling reveals developmental relationship and distinct biological functions of CD16+ and CD16- monocyte subsets. BMC Genomics 2009 Aug 27;10:403. PMID: 19712453
  • Microarray
    Affymetrix HG-U133_Plus_2
    8 Samples Loaded: 8
    Human (Homo sapiens)
    Monocytes
    Healthy
    Sample Set Spreadsheet
    Please log in in order to upload files.
    Samples Preview
    Sample ID !Sample_title cell type tissue cd16 donor health
    GSM422109 Peripheral blood monocytes, CD16 negative, rep1 monocyte peripheral blood negative healthy
    GSM422110 Peripheral blood monocytes, CD16 negative, rep2 monocyte peripheral blood negative healthy
    GSM422111 Peripheral blood monocytes, CD16 negative, rep3 monocyte peripheral blood negative healthy
    GSM422112 Peripheral blood monocytes, CD16 negative, rep4 monocyte peripheral blood negative healthy
    GSM422113 Peripheral blood monocytes, CD16 positive, rep1 monocyte peripheral blood positive healthy
    Please log in in order to upload files.
    File
    sampleset4000065_sampleannotations.csv
    Sample Set Spreadsheet
    Raw Signal
    Raw Signal
    •   |  View in GXB »
    Clinical Datasource
    Links

    Samples Viewer / Editor

    All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

    Sample ID !Sample Title Cell type Tissue Cd16 Donor health Phenotype
    GSM422109
    Peripheral blood monocytes, CD16 negative, rep1
    monocyte
    peripheral blood
    negative
    healthy
    CD16neg
    GSM422110
    Peripheral blood monocytes, CD16 negative, rep2
    monocyte
    peripheral blood
    negative
    healthy
    CD16neg
    GSM422111
    Peripheral blood monocytes, CD16 negative, rep3
    monocyte
    peripheral blood
    negative
    healthy
    CD16neg
    GSM422112
    Peripheral blood monocytes, CD16 negative, rep4
    monocyte
    peripheral blood
    negative
    healthy
    CD16neg
    GSM422113
    Peripheral blood monocytes, CD16 positive, rep1
    monocyte
    peripheral blood
    positive
    healthy
    CD16pos
    GSM422114
    Peripheral blood monocytes, CD16 positive, rep2
    monocyte
    peripheral blood
    positive
    healthy
    CD16pos
    GSM422115
    Peripheral blood monocytes, CD16 positive, rep3
    monocyte
    peripheral blood
    positive
    healthy
    CD16pos
    GSM422116
    Peripheral blood monocytes, CD16 positive, rep4
    monocyte
    peripheral blood
    positive
    healthy
    CD16pos

    Group Sets View in Gene Expression Browser

    Name  
    All Samples   View
    Monocyte subsets Default  View
    In order to make new Group Sets or change settings, please log in.

    Module Analysis

    Group Set  
    All Samples
    Monocyte subsets
    Your bug report has been sent!