GM-CSF induced gene-regulation in human monocytes-GSE63662
Human and murine studies showed that granulocyte macrophage colony-stimulating factor (GM-CSF) exerts beneficial effects in intestinal inflammation. To explore whether GM-CSF mediates its effects via monocytes, we analyzed effects of GM-CSF on monocytes in vitro and assessed the immunomodulatory potential of GM-CSF-activated monocytes (GMaM). We used microarray technology and functional assays to characterize GMaM in vitro and used a mouse model of colitis to study GMaM functions in vivo.
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Peripheral blood monocytes where cultured 16 h with media containing AB serum (control monocytes) or media containing 10 ng/mL GM-CSF and AB serum (GM-CSF activated monocytes). In three independent experiments, total RNA from GMaM and control monocytes was isolated and processed for microarray hybridization.
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Sample ID | !Sample_title | cell type | Culture conditions |
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GSM1554899 | Control monocytes 1 | isolated monocytes | Control |
GSM1554900 | GM-CSF activated monocytes 1 | isolated monocytes | GM-CSF |
GSM1554901 | Control monocytes 2 | isolated monocytes | Control |
GSM1554902 | GM-CSF activated monocytes 2 | isolated monocytes | GM-CSF |
GSM1554903 | Control monocytes 3 | isolated monocytes | Control |
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Sample ID | !Sample Title | Cell type | Culture conditions |
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GSM1554899 | Control monocytes 1 |
isolated monocytes |
Control |
GSM1554900 | GM-CSF activated monocytes 1 |
isolated monocytes |
GM-CSF |
GSM1554901 | Control monocytes 2 |
isolated monocytes |
Control |
GSM1554902 | GM-CSF activated monocytes 2 |
isolated monocytes |
GM-CSF |
GSM1554903 | Control monocytes 3 |
isolated monocytes |
Control |
GSM1554904 | GM-CSF activated monocytes 3 |
isolated monocytes |
GM-CSF |
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