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Clinical

GM-CSF induced gene-regulation in human monocytes-GSE63662

Purpose

Human and murine studies showed that granulocyte macrophage colony-stimulating factor (GM-CSF) exerts beneficial effects in intestinal inflammation. To explore whether GM-CSF mediates its effects via monocytes, we analyzed effects of GM-CSF on monocytes in vitro and assessed the immunomodulatory potential of GM-CSF-activated monocytes (GMaM). We used microarray technology and functional assays to characterize GMaM in vitro and used a mouse model of colitis to study GMaM functions in vivo.

Hypothesis

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Experimental Design

Peripheral blood monocytes where cultured 16 h with media containing AB serum (control monocytes) or media containing 10 ng/mL GM-CSF and AB serum (GM-CSF activated monocytes). In three independent experiments, total RNA from GMaM and control monocytes was isolated and processed for microarray hybridization.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Däbritz J, Weinhage T, Varga G, Wirth T et al. Reprogramming of monocytes by GM-CSF contributes to regulatory immune functions during intestinal inflammation. J Immunol 2015 Mar 1;194(5):2424-38. PMID: 25653427
  • Microarray
    Affymetrix HG-U133_Plus_2
    6 Samples Loaded: 6
    Human (Homo sapiens)
    Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title cell type Culture conditions
    GSM1554899 Control monocytes 1 isolated monocytes Control
    GSM1554900 GM-CSF activated monocytes 1 isolated monocytes GM-CSF
    GSM1554901 Control monocytes 2 isolated monocytes Control
    GSM1554902 GM-CSF activated monocytes 2 isolated monocytes GM-CSF
    GSM1554903 Control monocytes 3 isolated monocytes Control
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    File
    sampleset4000062_sampleannotations.csv
    Sample Set Spreadsheet
    Raw Signal
    Raw Signal
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    Sample ID !Sample Title Cell type Culture conditions
    GSM1554899
    Control monocytes 1
    isolated monocytes
    Control
    GSM1554900
    GM-CSF activated monocytes 1
    isolated monocytes
    GM-CSF
    GSM1554901
    Control monocytes 2
    isolated monocytes
    Control
    GSM1554902
    GM-CSF activated monocytes 2
    isolated monocytes
    GM-CSF
    GSM1554903
    Control monocytes 3
    isolated monocytes
    Control
    GSM1554904
    GM-CSF activated monocytes 3
    isolated monocytes
    GM-CSF

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