Human blood monocyte response to IL-17A in culture
The levels of IL-17 are elevated in the serum of psoriasis patients. However, the effects of IL-17 on circulating leukocytes bearing the IL-17 receptors are not fully understood. Of particular interest are monocytes, as the activation and recruitment of effector monocytes underlies the pathobiology of a number systemic inflammatory diseases, including psoriasis co-morbidities, such as atherosclerosis, metabolic regulation in adipose tissue and psoriatic arthritis. Human monocytes highly express both IL-17RA and IL-17RC and chemotraffick in response to IL-17. We explored the impact of IL-17 on blood monocytes. Using cDNA microarray, we molecularly characterized how human blood monocytes respond to IL-17A in vitro.
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Total RNA was extratced from CD14+ monocytes (isolated from human blood) after 24hr culture with or without IL-17A (200ng/mL)
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Suárez-Fariñas M, Wang CQ, Nograles KE, Krueger JG
| Sample ID | !Sample_title | cell type | tissue | treated with | subject |
|---|---|---|---|---|---|
| GSM1326112 | Sample 1_B584 | CD14+ monocytes | blood (buffy coat) | IL-17A (200ng/ml) for 24hrs in culture | healthy volunteer |
| GSM1326113 | Sample 2_B584 | CD14+ monocytes | blood (buffy coat) | none (control) | healthy volunteer |
| GSM1326114 | Sample 3_B585 | CD14+ monocytes | blood (buffy coat) | IL-17A (200ng/ml) for 24hrs in culture | healthy volunteer |
| GSM1326115 | Sample 4_B585 | CD14+ monocytes | blood (buffy coat) | none (control) | healthy volunteer |
| GSM1326116 | Sample 5_B586 | CD14+ monocytes | blood (buffy coat) | IL-17A (200ng/ml) for 24hrs in culture | healthy volunteer |
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| Sample ID | !Sample Title | Cell type | Tissue | Treated with | Subject |
|---|---|---|---|---|---|
| GSM1326112 | Sample 1_B584 |
CD14+ monocytes |
blood (buffy coat) |
IL-17A (200ng/ml) for 24hrs in culture |
healthy volunteer |
| GSM1326113 | Sample 2_B584 |
CD14+ monocytes |
blood (buffy coat) |
none (control) |
healthy volunteer |
| GSM1326114 | Sample 3_B585 |
CD14+ monocytes |
blood (buffy coat) |
IL-17A (200ng/ml) for 24hrs in culture |
healthy volunteer |
| GSM1326115 | Sample 4_B585 |
CD14+ monocytes |
blood (buffy coat) |
none (control) |
healthy volunteer |
| GSM1326116 | Sample 5_B586 |
CD14+ monocytes |
blood (buffy coat) |
IL-17A (200ng/ml) for 24hrs in culture |
healthy volunteer |
| GSM1326117 | Sample 6_B586 |
CD14+ monocytes |
blood (buffy coat) |
none (control) |
healthy volunteer |
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