Sample Set Annotation Tool: Expression data from intermediate monocytes from healthy donors and autoimmune uveitis patients-GSE66936

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

To compare the gene expression profiling 1) on intermediate monocytes CD14++CD16+ monocytes between healthy donors and autoimmune uveitis patients and 2) among 3 monocyte subsets in health donors

Hypothesis

Experimental Design

Pheripharal blood from 5 autoimmune uveitis patients and 4 healthy donors were collected. Immediately after collection, we purified circulating intermediate CD14++CD16+ monocytes from 5 patients with autoimmune uveitis ( Sample 13-17; titled as P intermediate 1-5) and 4 healthy donors (Sample 1, 4, 7, 10; titled as HD intermediate 1-4) by flow cytometry and isolated total RNA to proceed microarray assay. In addition, we also purified CD14+CD16++ (Sample 3, 6, 9, 12; titled HD non-classical 1-4) and CD14++CD16- (Sample 2, 5, 8, 11; titled HD classical 1-4) from 4 healthy donors to do microarray.

Experimental Variables

Please note that data processing was performed in two groups;[1] HD intermediate, classical and non-classical samples (total 12 samples)[2] HD intermediate samples reanalyzed with P intermediate samples (total 9 samples).

Controls

Methods

Additional Information

Liu B, Dhanda A, Hirani S, Williams EL et al. CD14++CD16+ Monocytes Are Enriched by Glucocorticoid Treatment and Are Functionally Attenuated in Driving Effector T Cell Responses. J Immunol 2015 Jun 1;194(11):5150-60. PMID: 25911752

Expected Samples:

21 Samples Loaded: 21

Species

Human (Homo sapiens)

Sample Source

Monocytes

Disease

Autoimmune uveitis patient

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	tissue	tissue subtype	subject id	subject status
GSM1634594	HD intermediate 1	human peripheral monocytes	intermediate monocyte subset	donor1	healthy donor
GSM1634595	HD classical 1	human peripheral monocytes	classical monocyte subset	donor1	healthy donor
GSM1634596	HD non-classical 1	human peripheral monocytes	non-classical monocyte subset	donor1	healthy donor
GSM1634597	HD intermediate 2	human peripheral monocytes	intermediate monocyte subset	donor2	healthy donor
GSM1634598	HD classical 2	human peripheral monocytes	classical monocyte subset	donor2	healthy donor

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File
sampleset4000056_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	!Sample Title	Tissue	Tissue subtype	Subject id	Subject status	Monocyte subsets
GSM1634594	HD intermediate 1	human peripheral monocytes	intermediate monocyte subset	donor1	healthy donor	HD intermediate
GSM1634595	HD classical 1	human peripheral monocytes	classical monocyte subset	donor1	healthy donor	HD classical
GSM1634596	HD non-classical 1	human peripheral monocytes	non-classical monocyte subset	donor1	healthy donor	HD non-classical
GSM1634597	HD intermediate 2	human peripheral monocytes	intermediate monocyte subset	donor2	healthy donor	HD intermediate
GSM1634598	HD classical 2	human peripheral monocytes	classical monocyte subset	donor2	healthy donor	HD classical
GSM1634599	HD non-classical 2	human peripheral monocytes	non-classical monocyte subset	donor2	healthy donor	HD non-classical
GSM1634600	HD intermediate 3	human peripheral monocytes	intermediate monocyte subset	donor3	healthy donor	HD intermediate
GSM1634601	HD classical 3	human peripheral monocytes	classical monocyte subset	donor3	healthy donor	HD classical
GSM1634602	HD non-classical 3	human peripheral monocytes	non-classical monocyte subset	donor3	healthy donor	HD non-classical
GSM1634603	HD intermediate 4	human peripheral monocytes	intermediate monocyte subset	donor4	healthy donor	HD intermediate
GSM1634604	HD classical 4	human peripheral monocytes	classical monocyte subset	donor4	healthy donor	HD classical
GSM1634605	HD non-classical 4	human peripheral monocytes	non-classical monocyte subset	donor4	healthy donor	HD non-classical
GSM1634606	HD intermediate 1 [re-analysis]	human peripheral monocytes	intermediate monocyte subset	donor1	healthy donor	HD intermediate
GSM1634607	HD intermediate 2 [re-analysis]	human peripheral monocytes	intermediate monocyte subset	donor2	healthy donor	HD intermediate
GSM1634608	HD intermediate 3 [re-analysis]	human peripheral monocytes	intermediate monocyte subset	donor3	healthy donor	HD intermediate
GSM1634609	HD intermediate 4 [re-analysis]	human peripheral monocytes	intermediate monocyte subset	donor4	healthy donor	HD intermediate
GSM1634610	P intermediate 1	human peripheral monocytes	intermediate monocyte subset	patient 1	autoimmune uveitis patient	P intermediate
GSM1634611	P intermediate 2	human peripheral monocytes	intermediate monocyte subset	patient 2	autoimmune uveitis patient	P intermediate
GSM1634612	P intermediate 3	human peripheral monocytes	intermediate monocyte subset	patient 3	autoimmune uveitis patient	P intermediate
GSM1634613	P intermediate 4	human peripheral monocytes	intermediate monocyte subset	patient 4	autoimmune uveitis patient	P intermediate
GSM1634614	P intermediate 5	human peripheral monocytes	intermediate monocyte subset	patient 5	autoimmune uveitis patient	P intermediate

Group Sets View in Gene Expression Browser

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Monocyte subsets	Default	View
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Module Analysis

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Monocyte subsets

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