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Clinical

Comparative analysis of monocytes from healthy donors, patients with metastatic breast cancer, sepsis or tuberculosis-GSE65517

Purpose

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Hypothesis

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Experimental Design

Peripheral blood monocytes were isolated using magnetic cell sorting from 4 patients with metastatic breast cancer, 3 healthy controls, 3 patients with sepsis and 3 patients with active tuberculosis were immediately frozen at -80°C in TRIZOL.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Bergenfelz C, Larsson AM, von Stedingk K, Gruvberger-Saal S et al. Systemic Monocytic-MDSCs Are Generated from Monocytes and Correlate with Disease Progression in Breast Cancer Patients. PLoS One 2015;10(5):e0127028. PMID: 25992611
  • Microarray
    Illumina HumanHT-12 v4
    13 Samples Loaded: 13
    Human (Homo sapiens)
    Monocytes
    Breast Cancer & Bacterial infection
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title cell type disease state
    GSM1599177 Primary human monocytes from patient with metastatic breast cancer #1 primary monocyte Metastatic breast cancer
    GSM1599178 Primary human monocytes from patient with metastatic breast cancer #2 primary monocyte Metastatic breast cancer
    GSM1599179 Primary human monocytes from patient with metastatic breast cancer #3 primary monocyte Metastatic breast cancer
    GSM1599180 Primary human monocytes from patient with metastatic breast cancer #4 primary monocyte Metastatic breast cancer
    GSM1599181 Primary human monocytes from healthy control #1 primary monocyte Healthy control
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    Raw Signal
    Raw Signal
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    Sample ID !Sample Title Cell type Disease state
    GSM1599177
    Primary human monocytes from patient with metastatic breast cancer #1
    primary monocyte
    Metastatic breast cancer
    GSM1599178
    Primary human monocytes from patient with metastatic breast cancer #2
    primary monocyte
    Metastatic breast cancer
    GSM1599179
    Primary human monocytes from patient with metastatic breast cancer #3
    primary monocyte
    Metastatic breast cancer
    GSM1599180
    Primary human monocytes from patient with metastatic breast cancer #4
    primary monocyte
    Metastatic breast cancer
    GSM1599181
    Primary human monocytes from healthy control #1
    primary monocyte
    Healthy control
    GSM1599182
    Primary human monocytes from healthy control #2
    primary monocyte
    Healthy control
    GSM1599183
    Primary human monocytes from healthy control #3
    primary monocyte
    Healthy control
    GSM1599184
    Primary human monocytes from patient with gram-negative sepsis #1
    primary monocyte
    Gram negative sepsis
    GSM1599185
    Primary human monocytes from patient with gram-negative sepsis #2
    primary monocyte
    Gram negative sepsis
    GSM1599186
    Primary human monocytes from patient with gram-negative sepsis #3
    primary monocyte
    Gram negative sepsis
    GSM1599187
    Primary human monocytes from patient with tuberculosis #1
    primary monocyte
    Tuberculosis
    GSM1599188
    Primary human monocytes from patient with tuberculosis #2
    primary monocyte
    Tuberculosis
    GSM1599189
    Primary human monocytes from patient with tuberculosis #3
    primary monocyte
    Tuberculosis

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