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Clinical

Comparison study between Uremic patient with Healthy control-GSE43484

Purpose

To explore the gene expression profile and to identify differences in activated pathways in monocytes relevant to the pathophysiology of atherosclerosis and increased susceptibility to infections.

Hypothesis

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Experimental Design

Monocytes from CKD patients (stages 4 and 5, eGFR <20 ml/min x 1.73 m2) and healthy donors were collected from peripheral blood. Microarray gene expression profile was done and data were interpreted by Genespring software and Panther tools website.

Experimental Variables

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Controls

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Methods

Six gene expression Affymetrix chip were used. Three for each group

Additional Information

Al-Chaqmaqchi H, Moshfegh A, Dadfar E, Paulsson J, Hassan M, Jacobson SH,Lundahl J

Microarray
Affymetrix HG-U133A
6 Samples Loaded: 6
Human (Homo sapiens)
Monocytes
Uremic
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample_title cell type subject
GSM1063568 Uremic Monocyte rep1 CD14 purified perpheral blood monocytes Uremic patient
GSM1063569 Uremic Monocyte rep2 CD14 purified perpheral blood monocytes Uremic patient
GSM1063570 Uremic Monocyte rep3 CD14 purified perpheral blood monocytes Uremic patient
GSM1063571 Healthy Monocyte rep1 CD14 purified perpheral blood monocytes healthy control
GSM1063572 Healthy Monocyte rep2 CD14 purified perpheral blood monocytes healthy control
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Raw Signal
Raw Signal
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Sample ID !Sample Title Cell type Subject
GSM1063568
Uremic Monocyte rep1
CD14 purified perpheral blood monocytes
Uremic patient
GSM1063569
Uremic Monocyte rep2
CD14 purified perpheral blood monocytes
Uremic patient
GSM1063570
Uremic Monocyte rep3
CD14 purified perpheral blood monocytes
Uremic patient
GSM1063571
Healthy Monocyte rep1
CD14 purified perpheral blood monocytes
healthy control
GSM1063572
Healthy Monocyte rep2
CD14 purified perpheral blood monocytes
healthy control
GSM1063573
Healthy Monocyte rep3
CD14 purified perpheral blood monocytes
healthy control

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