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Clinical

mRNA expression profiling of human immune cell subsets (HUG)-GSE28491

Purpose

Blood consists of different cell populations with distinct functions and correspondingly, distinct gene expression profiles. In this study, global miRNA expression profiling was performed across a panel of nine human immune cell subsets (neutrophils, eosinophils, monocytes, B cells, NK cells, CD4 T cells, CD8 T cells, mDCs and pDCs) to identify cell-type specific miRNAs. mRNA expression profiling was performed on the same samples, to determine if miRNAs specific to certain cell types down-regulated expression levels of their target genes.

Hypothesis

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Experimental Design

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Experimental Variables

Nine cell subsets (CD16+CD66b+ Neutrophils, CD16-CD66b+ Eosinophils, CD14+ Monocytes, CD4+ T cells, CD8+ T cells, CD56+ NK cells, CD19+ B cells, CD123+ pDCs and CD11c+ mDCs) were isolated from healthy human blood and assessed for cell type purity by flow cytometry. Cell types were isolated from 5 pools of 5 healthy donors each, with the exception for monocytes, where 10 pools were used. RNA obtained from these purified populations were subsequently used for miRNA and mRNA expression profiling by Affymetrix GeneChip miRNA and HG-U133Plus2.0 microarrays.

Controls

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Methods

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Additional Information
  • Allantaz F, Cheng DT, Bergauer T, Ravindran P et al. Expression profiling of human immune cell subsets identifies miRNA-mRNA regulatory relationships correlated with cell type specific expression. PLoS One 2012;7(1):e29979. PMID: 22276136
  • Microarray
    Affymetrix HG-U133_Plus_2
    33 Samples Loaded: 33
    Human (Homo sapiens)
    CD4 T cells , NK Cells , CD8 T cells , Eosinophils , B cells , Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title cell type
    GSM705402 B cells rep1 mRNA (HUG) CD19+ B cells
    GSM705403 B cells rep2 mRNA (HUG) CD19+ B cells
    GSM705404 B cells rep3 mRNA (HUG) CD19+ B cells
    GSM705405 B cells rep4 mRNA (HUG) CD19+ B cells
    GSM705406 B cells rep5 mRNA (HUG) CD19+ B cells
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    Log2 Transformed
    Raw Signal
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    Sample ID !Sample Title Cell type
    GSM705402
    B cells rep1 mRNA (HUG)
    CD19+ B cells
    GSM705403
    B cells rep2 mRNA (HUG)
    CD19+ B cells
    GSM705404
    B cells rep3 mRNA (HUG)
    CD19+ B cells
    GSM705405
    B cells rep4 mRNA (HUG)
    CD19+ B cells
    GSM705406
    B cells rep5 mRNA (HUG)
    CD19+ B cells
    GSM705407
    Monocytes rep1 mRNA (HUG)
    CD14+ monocytes
    GSM705408
    Monocytes rep2 mRNA (HUG)
    CD14+ monocytes
    GSM705409
    Monocytes rep3 mRNA (HUG)
    CD14+ monocytes
    GSM705410
    Monocytes rep4 mRNA (HUG)
    CD14+ monocytes
    GSM705411
    Monocytes rep5 mRNA (HUG)
    CD14+ monocytes
    GSM705412
    CD4+ T cells rep1 mRNA (HUG)
    CD4+ T cells
    GSM705413
    CD4+ T cells rep2 mRNA (HUG)
    CD4+ T cells
    GSM705414
    CD4+ T cells rep3 mRNA (HUG)
    CD4+ T cells
    GSM705415
    CD4+ T cells rep4 mRNA (HUG)
    CD4+ T cells
    GSM705416
    CD4+ T cells rep5 mRNA (HUG)
    CD4+ T cells
    GSM705417
    CD8+ T cells rep1 mRNA (HUG)
    CD8+ T cells
    GSM705418
    CD8+ T cells rep2 mRNA (HUG)
    CD8+ T cells
    GSM705419
    CD8+ T cells rep3 mRNA (HUG)
    CD8+ T cells
    GSM705420
    CD8+ T cells rep4 mRNA (HUG)
    CD8+ T cells
    GSM705421
    CD8+ T cells rep5 mRNA (HUG)
    CD8+ T cells
    GSM705422
    Eosinophils rep1 mRNA (HUG)
    Eosinophils
    GSM705423
    Eosinophils rep2 mRNA (HUG)
    Eosinophils
    GSM705424
    Eosinophils rep3 mRNA (HUG)
    Eosinophils
    GSM705425
    NK cells rep1 mRNA (HUG)
    NK cells
    GSM705426
    NK cells rep2 mRNA (HUG)
    NK cells
    GSM705427
    NK cells rep3 mRNA (HUG)
    NK cells
    GSM705428
    NK cells rep4 mRNA (HUG)
    NK cells
    GSM705429
    NK cells rep5 mRNA (HUG)
    NK cells
    GSM705430
    Neutrophils rep1 mRNA (HUG)
    Neutrophils
    GSM705431
    Neutrophils rep2 mRNA (HUG)
    Neutrophils
    GSM705432
    Neutrophils rep3 mRNA (HUG)
    Neutrophils
    GSM705433
    Neutrophils rep4 mRNA (HUG)
    Neutrophils
    GSM705434
    Neutrophils rep5 mRNA (HUG)
    Neutrophils

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