mRNA expression profiling of human immune cell subsets (HUG)-GSE28491
Blood consists of different cell populations with distinct functions and correspondingly, distinct gene expression profiles. In this study, global miRNA expression profiling was performed across a panel of nine human immune cell subsets (neutrophils, eosinophils, monocytes, B cells, NK cells, CD4 T cells, CD8 T cells, mDCs and pDCs) to identify cell-type specific miRNAs. mRNA expression profiling was performed on the same samples, to determine if miRNAs specific to certain cell types down-regulated expression levels of their target genes.
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Nine cell subsets (CD16+CD66b+ Neutrophils, CD16-CD66b+ Eosinophils, CD14+ Monocytes, CD4+ T cells, CD8+ T cells, CD56+ NK cells, CD19+ B cells, CD123+ pDCs and CD11c+ mDCs) were isolated from healthy human blood and assessed for cell type purity by flow cytometry. Cell types were isolated from 5 pools of 5 healthy donors each, with the exception for monocytes, where 10 pools were used. RNA obtained from these purified populations were subsequently used for miRNA and mRNA expression profiling by Affymetrix GeneChip miRNA and HG-U133Plus2.0 microarrays.
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Sample ID | !Sample_title | cell type |
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GSM705402 | B cells rep1 mRNA (HUG) | CD19+ B cells |
GSM705403 | B cells rep2 mRNA (HUG) | CD19+ B cells |
GSM705404 | B cells rep3 mRNA (HUG) | CD19+ B cells |
GSM705405 | B cells rep4 mRNA (HUG) | CD19+ B cells |
GSM705406 | B cells rep5 mRNA (HUG) | CD19+ B cells |
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Sample ID | !Sample Title | Cell type |
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GSM705402 | B cells rep1 mRNA (HUG) |
CD19+ B cells |
GSM705403 | B cells rep2 mRNA (HUG) |
CD19+ B cells |
GSM705404 | B cells rep3 mRNA (HUG) |
CD19+ B cells |
GSM705405 | B cells rep4 mRNA (HUG) |
CD19+ B cells |
GSM705406 | B cells rep5 mRNA (HUG) |
CD19+ B cells |
GSM705407 | Monocytes rep1 mRNA (HUG) |
CD14+ monocytes |
GSM705408 | Monocytes rep2 mRNA (HUG) |
CD14+ monocytes |
GSM705409 | Monocytes rep3 mRNA (HUG) |
CD14+ monocytes |
GSM705410 | Monocytes rep4 mRNA (HUG) |
CD14+ monocytes |
GSM705411 | Monocytes rep5 mRNA (HUG) |
CD14+ monocytes |
GSM705412 | CD4+ T cells rep1 mRNA (HUG) |
CD4+ T cells |
GSM705413 | CD4+ T cells rep2 mRNA (HUG) |
CD4+ T cells |
GSM705414 | CD4+ T cells rep3 mRNA (HUG) |
CD4+ T cells |
GSM705415 | CD4+ T cells rep4 mRNA (HUG) |
CD4+ T cells |
GSM705416 | CD4+ T cells rep5 mRNA (HUG) |
CD4+ T cells |
GSM705417 | CD8+ T cells rep1 mRNA (HUG) |
CD8+ T cells |
GSM705418 | CD8+ T cells rep2 mRNA (HUG) |
CD8+ T cells |
GSM705419 | CD8+ T cells rep3 mRNA (HUG) |
CD8+ T cells |
GSM705420 | CD8+ T cells rep4 mRNA (HUG) |
CD8+ T cells |
GSM705421 | CD8+ T cells rep5 mRNA (HUG) |
CD8+ T cells |
GSM705422 | Eosinophils rep1 mRNA (HUG) |
Eosinophils |
GSM705423 | Eosinophils rep2 mRNA (HUG) |
Eosinophils |
GSM705424 | Eosinophils rep3 mRNA (HUG) |
Eosinophils |
GSM705425 | NK cells rep1 mRNA (HUG) |
NK cells |
GSM705426 | NK cells rep2 mRNA (HUG) |
NK cells |
GSM705427 | NK cells rep3 mRNA (HUG) |
NK cells |
GSM705428 | NK cells rep4 mRNA (HUG) |
NK cells |
GSM705429 | NK cells rep5 mRNA (HUG) |
NK cells |
GSM705430 | Neutrophils rep1 mRNA (HUG) |
Neutrophils |
GSM705431 | Neutrophils rep2 mRNA (HUG) |
Neutrophils |
GSM705432 | Neutrophils rep3 mRNA (HUG) |
Neutrophils |
GSM705433 | Neutrophils rep4 mRNA (HUG) |
Neutrophils |
GSM705434 | Neutrophils rep5 mRNA (HUG) |
Neutrophils |
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