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Clinical

mRNA expression profiling of human immune cell subset (Roche)-GSE28490

Purpose

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Hypothesis

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Experimental Design

Nine cell subsets (CD16+CD66b+ Neutrophils, CD16-CD66b+ Eosinophils, CD14+ Monocytes, CD4+ T cells, CD8+ T cells, CD56+ NK cells, CD19+ B cells, CD123+ pDCs and CD11c+ mDCs) were isolated from healthy human blood and assessed for cell type purity by flow cytometry. Cell types were isolated from 5 pools of 5 healthy donors each, with the exception for monocytes, where 10 pools were used. RNA obtained from these purified populations were subsequently used for miRNA and mRNA expression profiling by Affymetrix GeneChip miRNA and HG-U133Plus2.0 microarrays.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Allantaz F, Cheng DT, Bergauer T, Ravindran P et al. Expression profiling of human immune cell subsets identifies miRNA-mRNA regulatory relationships correlated with cell type specific expression. PLoS One 2012;7(1):e29979. PMID: 22276136
  • Microarray
    Affymetrix HG-U133_Plus_2
    47 Samples Loaded: 47
    Human (Homo sapiens)
    mDC , Neutrophils , Monocytes , CD4 T cells , pDC , CD8 T cells , Eosinophils , T cells , NK Cells , Dendritic Cells
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title cell type
    GSM705287 Monocytes rep1 mRNA (Roche) Monocytes
    GSM705288 Monocytes rep2 mRNA (Roche) Monocytes
    GSM705289 Monocytes rep3 mRNA (Roche) Monocytes
    GSM705290 Monocytes rep4 mRNA (Roche) Monocytes
    GSM705291 Monocytes rep5 mRNA (Roche) Monocytes
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    File
    Log2 Transformed
    Raw Signal
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    Sample ID !Sample Title Cell type
    GSM705287
    Monocytes rep1 mRNA (Roche)
    Monocytes
    GSM705288
    Monocytes rep2 mRNA (Roche)
    Monocytes
    GSM705289
    Monocytes rep3 mRNA (Roche)
    Monocytes
    GSM705290
    Monocytes rep4 mRNA (Roche)
    Monocytes
    GSM705291
    Monocytes rep5 mRNA (Roche)
    Monocytes
    GSM705292
    Monocytes rep6 mRNA (Roche)
    Monocytes
    GSM705293
    Monocytes rep7 mRNA (Roche)
    Monocytes
    GSM705294
    Monocytes rep8 mRNA (Roche)
    Monocytes
    GSM705295
    Monocytes rep9 mRNA (Roche)
    Monocytes
    GSM705296
    Monocytes rep10 mRNA (Roche)
    Monocytes
    GSM705297
    B cells rep1 mRNA (Roche)
    B cells
    GSM705298
    B cells rep2 mRNA (Roche)
    B cells
    GSM705299
    B cells rep3 mRNA (Roche)
    B cells
    GSM705300
    B cells rep4 mRNA (Roche)
    B cells
    GSM705301
    B cells rep5 mRNA (Roche)
    B cells
    GSM705302
    CD4+ T cells rep1 mRNA (Roche)
    CD4+ T cells
    GSM705303
    CD4+ T cells rep2 mRNA (Roche)
    CD4+ T cells
    GSM705304
    CD4+ T cells rep3 mRNA (Roche)
    CD4+ T cells
    GSM705305
    CD4+ T cells rep4 mRNA (Roche)
    CD4+ T cells
    GSM705306
    CD4+ T cells rep5 mRNA (Roche)
    CD4+ T cells
    GSM705307
    NK cells rep1 mRNA (Roche)
    NK cells
    GSM705308
    NK cells rep2 mRNA (Roche)
    NK cells
    GSM705309
    NK cells rep3 mRNA (Roche)
    NK cells
    GSM705310
    NK cells rep4 mRNA (Roche)
    NK cells
    GSM705311
    NK cells rep5 mRNA (Roche)
    NK cells
    GSM705312
    CD8+ T cells rep1 mRNA (Roche)
    CD8+ T cells
    GSM705313
    CD8+ T cells rep2 mRNA (Roche)
    CD8+ T cells
    GSM705314
    CD8+ T cells rep3 mRNA (Roche)
    CD8+ T cells
    GSM705315
    CD8+ T cells rep4 mRNA (Roche)
    CD8+ T cells
    GSM705316
    CD8+ T cells rep5 mRNA (Roche)
    CD8+ T cells
    GSM705317
    Eosinophils rep1 mRNA (Roche)
    Eosinophils
    GSM705318
    Eosinophils rep2 mRNA (Roche)
    Eosinophils
    GSM705319
    Eosinophils rep3 mRNA (Roche)
    Eosinophils
    GSM705320
    Eosinophils rep4 mRNA (Roche)
    Eosinophils
    GSM705321
    mDC rep1 mRNA (Roche)
    mDCs
    GSM705322
    mDC rep2 mRNA (Roche)
    mDCs
    GSM705323
    mDC rep3 mRNA (Roche)
    mDCs
    GSM705324
    mDC rep4 mRNA (Roche)
    mDCs
    GSM705325
    mDC rep5 mRNA (Roche)
    mDCs
    GSM705326
    Neutrophils rep1 mRNA (Roche)
    Neutrophils
    GSM705327
    Neutrophils rep2 mRNA (Roche)
    Neutrophils
    GSM705328
    Neutrophils rep3 mRNA (Roche)
    Neutrophils
    GSM705329
    pDC rep1 mRNA (Roche)
    pDCs
    GSM705330
    pDC rep2 mRNA (Roche)
    pDCs
    GSM705331
    pDC rep3 mRNA (Roche)
    pDCs
    GSM705332
    pDC rep4 mRNA (Roche)
    pDCs
    GSM705333
    pDC rep5 mRNA (Roche)
    pDCs

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