Sample Set Annotation Tool: Direct Cell Conversion of Human Fibroblasts to Monocytic phagocytes by Forced Expression of Monocytic Regulatory Network Elements-GSE27304

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Identifying the key TFs of human monocyte by combining comparative gene expression analysis and literature based text-mining

Hypothesis

Experimental Design

Total RNA obtained from human dermal fibroblasts(FIB), human CD14+ monocytes(MON), mock lentivirus vector transduced fibroblasts (FIB-mock), SPI1 transduced fibroblasts (FIB-SPI1), and SPI1, CEBPA, MNDA, IRF8 transduced fibroblasts(FIB-4Fs). The fold change was computed compared with fibroblasts or FIB-mock.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

20 Samples Loaded: 15

Species

Human (Homo sapiens)

Sample Source

Monocytes , Fibroblast

Disease

Dermatomyositis Polymyositis

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	Gender	!Sample_title	cell line	tissue derived
GSM675105	Male	FIB_rep1	fibroblast	skin
GSM675106	Male	FIB_rep2	fibroblast	skin
GSM675107	Male	FIB_rep3	fibroblast	skin
GSM675108	Male	MON_rep1	CD14+ cells	Peripheral blood
GSM675109	Male	MON_rep2	CD14+ cells	Peripheral blood

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File

Samples Viewer / Editor

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Sample ID	Gender	!Sample Title	Cell line	Tissue derived
GSM675105	Male	FIB_rep1	fibroblast	skin
GSM675106	Male	FIB_rep2	fibroblast	skin
GSM675107	Male	FIB_rep3	fibroblast	skin
GSM675108	Male	MON_rep1	CD14+ cells	Peripheral blood
GSM675109	Male	MON_rep2	CD14+ cells	Peripheral blood
GSM675110	Male	MON_rep3	CD14+ cells	Peripheral blood
GSM675111	Male	FIB_mock_rep1	fibroblast	skin
GSM675112	Male	FIB_mock_rep2	fibroblast	skin
GSM675113	Male	FIB_mock_rep3	fibroblast	skin
GSM675114	Male	FIB_SPI1_rep1	fibroblast	skin
GSM675115	Male	FIB_SPI1_rep2	fibroblast	skin
GSM675116	Male	FIB_SPI1_rep3	fibroblast	skin
GSM675117	Male	FIB-4Fs_1	fibroblast	skin
GSM675118	Male	FIB-4Fs_2	fibroblast	skin
GSM675119	Male	FIB-4Fs_3	fibroblast	skin

Group Sets View in Gene Expression Browser

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Module Analysis

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SIDRA