Sample Set Annotation Tool: Direct Cell Conversion of Human Fibroblasts to Monocytic phagocytes by Forced Expression of Monocytic Regulatory Network Elements-GSE27304

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Identifying the key TFs of human monocyte by combining comparative gene expression analysis and literature based text-mining

Hypothesis

Experimental Design

Total RNA obtained from human dermal fibroblasts(FIB), human CD14+ monocytes(MON), mock lentivirus vector transduced fibroblasts (FIB-mock), SPI1 transduced fibroblasts (FIB-SPI1), and SPI1, CEBPA, MNDA, IRF8 transduced fibroblasts(FIB-4Fs). The fold change was computed compared with fibroblasts or FIB-mock.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

20 Samples Loaded: 15

Species

Human (Homo sapiens)

Sample Source

Fibroblast , Monocytes

Disease

Dermatomyositis Polymyositis

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

Please log in in order to upload files.

Samples Preview

Sample ID	Gender	!Sample_title	cell line	tissue derived
GSM675105	Male	FIB_rep1	fibroblast	skin
GSM675106	Male	FIB_rep2	fibroblast	skin
GSM675107	Male	FIB_rep3	fibroblast	skin
GSM675108	Male	MON_rep1	CD14+ cells	Peripheral blood
GSM675109	Male	MON_rep2	CD14+ cells	Peripheral blood

Please log in in order to upload files.

File

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	Gender	!Sample Title	Cell line	Tissue derived
GSM675105	Male	FIB_rep1	fibroblast	skin
GSM675106	Male	FIB_rep2	fibroblast	skin
GSM675107	Male	FIB_rep3	fibroblast	skin
GSM675108	Male	MON_rep1	CD14+ cells	Peripheral blood
GSM675109	Male	MON_rep2	CD14+ cells	Peripheral blood
GSM675110	Male	MON_rep3	CD14+ cells	Peripheral blood
GSM675111	Male	FIB_mock_rep1	fibroblast	skin
GSM675112	Male	FIB_mock_rep2	fibroblast	skin
GSM675113	Male	FIB_mock_rep3	fibroblast	skin
GSM675114	Male	FIB_SPI1_rep1	fibroblast	skin
GSM675115	Male	FIB_SPI1_rep2	fibroblast	skin
GSM675116	Male	FIB_SPI1_rep3	fibroblast	skin
GSM675117	Male	FIB-4Fs_1	fibroblast	skin
GSM675118	Male	FIB-4Fs_2	fibroblast	skin
GSM675119	Male	FIB-4Fs_3	fibroblast	skin

Group Sets View in Gene Expression Browser

Name
All Samples	Default	View
In order to make new Group Sets or change settings, please log in.

Module Analysis

Group Set
All Samples

SIDRA