Deconvoluting Early Post-Transplant Immunity Using Purified Cell Subsets Reveals Functional Networks Not Evident by Whole Blood Analysis-GSE24223
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We employed Affymetrix HG-U133 Plus 2.0 GeneChips for mRNA profiling of whole blood and purified CD4, CD8 T cells, B cells and monocytes in tandem with high-throughput flow cytometry in 10 kidney transplant patients sampled serially pre-transplant, 1, 2, 4, 8 and 12 weeks. We then mechanistically deconvoluted the early post-transplant immune response.
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Sample ID | !Sample_title | time point | cell subtype | donor number |
---|---|---|---|---|
GSM595693 | 00704_4 | Week 4 | CD4 | 7 |
GSM595694 | 0101-Pax | Pre-Tx | Whole Blood | 1 |
GSM595695 | 0102-Pax | Week 1 | Whole Blood | 1 |
GSM595697 | 0103-CD14_ | Week 2 | CD14 | 1 |
GSM595698 | 0103-CD19_ | Week 2 | CD19 | 1 |
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Sample ID | !Sample Title | Time point | Cell subtype | Donor number |
---|---|---|---|---|
GSM595693 | 00704_4 |
Week 4 |
CD4 |
7 |
GSM595694 | 0101-Pax |
Pre-Tx |
Whole Blood |
1 |
GSM595695 | 0102-Pax |
Week 1 |
Whole Blood |
1 |
GSM595697 | 0103-CD14_ |
Week 2 |
CD14 |
1 |
GSM595698 | 0103-CD19_ |
Week 2 |
CD19 |
1 |
GSM595700 | 0103-CD4_ |
Week 2 |
CD4 |
1 |
GSM595701 | 0103-CD8_ |
Week 2 |
CD8 |
1 |
GSM595702 | 0103-Pax |
Week 2 |
Whole Blood |
1 |
GSM595703 | 0104-Pax |
Week 4 |
Whole Blood |
1 |
GSM595704 | 0106-Pax |
Week 12 |
Whole Blood |
1 |
GSM595705 | 201 |
Pre-Tx |
Whole Blood |
2 |
GSM595706 | 0202-Pax |
Week 1 |
Whole Blood |
2 |
GSM595707 | 0203-CD14_ |
Week 2 |
CD14 |
2 |
GSM595708 | 0203-CD19_ |
Week 2 |
CD19 |
2 |
GSM595709 | 0203-CD4_ |
Week 2 |
CD4 |
2 |
GSM595710 | 204 |
Week 4 |
Whole Blood |
2 |
GSM595711 | 0205-Pax |
Week 8 |
Whole Blood |
2 |
GSM595712 | 0205_PBMC |
Week 12 |
Whole blood |
2 |
GSM595713 | 0206-CD14_ |
Week 12 |
CD14 |
2 |
GSM595714 | 0206-CD19_ |
Week 12 |
CD19 |
2 |
GSM595715 | 0206-CD4_ |
Week 12 |
CD4 |
2 |
GSM595716 | 0206-CD8_ |
Week 12 |
CD8 |
2 |
GSM595717 | 206 |
Week 12 |
Whole Blood |
2 |
GSM595718 | 0301-Pax |
Pre-Tx |
Whole Blood |
3 |
GSM595719 | 0303-CD14_ |
Week 2 |
CD14 |
3 |
GSM595720 | 0303-CD19_ |
Week 2 |
CD19 |
3 |
GSM595721 | 0303-CD4_ |
Week 2 |
CD4 |
3 |
GSM595722 | 0303-Pax |
Week 2 |
Whole Blood |
3 |
GSM595723 | 304 |
Week 4 |
Whole Blood |
3 |
GSM595724 | 305 |
Week 8 |
Whole Blood |
3 |
GSM595725 | 0306-CD14_ |
Week 12 |
CD14 |
3 |
GSM595726 | 0306-CD4_ |
Week 12 |
CD4 |
3 |
GSM595727 | 0306-CD8_ |
Week 12 |
CD8 |
3 |
GSM595728 | 0306-Pax |
Week 12 |
Whole Blood |
3 |
GSM595729 | 402 |
Week 1 |
Whole Blood |
4 |
GSM595730 | 0403-CD14_ |
Week 2 |
CD14 |
4 |
GSM595731 | 0403-CD8_ |
Week 2 |
CD8 |
4 |
GSM595732 | 403 |
Week 2 |
Whole Blood |
4 |
GSM595733 | 0404-Pax |
Week 4 |
Whole Blood |
4 |
GSM595734 | 0404_PBL |
Week 4 |
Whole blood |
4 |
GSM595735 | 405 |
Week 8 |
Whole Blood |
4 |
GSM595736 | 0405_14 |
Week 8 |
CD14 |
4 |
GSM595737 | 0405_8 |
Week 8 |
CD8 |
4 |
GSM595738 | 0405_PBL |
Week 8 |
Whole blood |
4 |
GSM595739 | 0406-CD14_ |
Week 12 |
CD14 |
4 |
GSM595740 | 0406-CD4_ |
Week 12 |
CD4 |
4 |
GSM595741 | 0406-CD8_ |
Week 12 |
CD8 |
4 |
GSM595742 | 406 |
Week 12 |
Whole Blood |
4 |
GSM595743 | 0406_14 |
Week 12 |
CD14 |
4 |
GSM595744 | 0406_4 |
Week 12 |
CD4 |
4 |
GSM595745 | 0501_14 |
Pre-Tx |
CD14 |
5 |
GSM595746 | 0501_PBMC |
Pre-Tx |
Whole blood |
5 |
GSM595747 | 502 |
Week 1 |
Whole Blood |
5 |
GSM595748 | 0503 CD14 |
Week 2 |
CD14 |
5 |
GSM595749 | 0503 CD4 |
Week 2 |
CD4 |
5 |
GSM595750 | 0503 CD56 |
Week 2 |
CD56 |
5 |
GSM595751 | 0503 CD8 |
Week 2 |
CD8 |
5 |
GSM595752 | 0503-CD14_ |
Week 2 |
CD14 |
5 |
GSM595753 | 0503-CD4_ |
Week 2 |
CD4 |
5 |
GSM595754 | 0503-CD8_ |
Week 2 |
CD8 |
5 |
GSM595755 | 0503-Pax |
Week 2 |
Whole Blood |
5 |
GSM595756 | 0504-Pax |
Week 4 |
Whole Blood |
5 |
GSM595757 | 506 |
Week 12 |
Whole Blood |
5 |
GSM595758 | 0601_14 |
Pre-Tx |
CD14 |
6 |
GSM595759 | 0601_4 |
Pre-Tx |
CD4 |
6 |
GSM595760 | 0601_PBL |
Pre-Tx |
Whole blood |
6 |
GSM595761 | 0602_14 |
Week 1 |
CD14 |
6 |
GSM595762 | 0602_PBL |
Week 1 |
Whole blood |
6 |
GSM595763 | 701 |
Pre-Tx |
Whole Blood |
7 |
GSM595764 | 0701_4 |
Pre-Tx |
CD4 |
7 |
GSM595765 | 0701_8 |
Pre-Tx |
CD8 |
7 |
GSM595766 | 702 |
Week 1 |
Whole Blood |
7 |
GSM595767 | 0702_14 |
Week 1 |
CD14 |
7 |
GSM595768 | 0702_4 |
Week 1 |
CD4 |
7 |
GSM595769 | 0702_PBL |
Week 1 |
Whole blood |
7 |
GSM595770 | 0703 CD14 |
Week 2 |
CD14 |
7 |
GSM595771 | 0703 CD4 |
Week 2 |
CD4 |
7 |
GSM595772 | 0703 CD8 |
Week 2 |
CD8 |
7 |
GSM595773 | 703 |
Week 2 |
Whole Blood |
7 |
GSM595774 | 704 |
Week 4 |
Whole Blood |
7 |
GSM595775 | 705 |
Week 8 |
Whole Blood |
7 |
GSM595776 | 0705_4 |
Week 8 |
CD4 |
7 |
GSM595777 | 0706 CD14 |
Week 12 |
CD14 |
7 |
GSM595778 | 0706 CD19 |
Week 12 |
CD19 |
7 |
GSM595779 | 0706 CD4 |
Week 12 |
CD4 |
7 |
GSM595780 | 0706 CD8 |
Week 12 |
CD8 |
7 |
GSM595781 | 0706-CD14_ |
Week 12 |
CD14 |
7 |
GSM595782 | 0706-CD4_ |
Week 12 |
CD4 |
7 |
GSM595783 | 0706-CD8_ |
Week 12 |
CD8 |
7 |
GSM595784 | 706 |
Week 12 |
Whole Blood |
7 |
GSM595785 | 0901_4 |
Pre-Tx |
CD4 |
9 |
GSM595786 | 1 CD14 |
Control Day 0 |
CD14 |
Control 1 |
GSM595787 | 1 CD19 |
Control Day 0 |
CD19 |
Control 1 |
GSM595788 | 1 CD4 |
Control Day 0 |
CD4 |
Control 1 |
GSM595789 | 1 CD56 |
Control Day 0 |
CD56 |
Control 1 |
GSM595790 | 1 CD8 |
Control Day 0 |
CD8 |
Control 1 |
GSM595791 | 1001 |
Pre-Tx |
Whole Blood |
10 |
GSM595792 | 1001_8 |
Pre-Tx |
CD8 |
10 |
GSM595793 | 1001_PBL |
Pre-Tx |
Whole blood |
10 |
GSM595794 | 1002 |
Week 1 |
Whole Blood |
10 |
GSM595795 | 1003 CD14 |
Week 2 |
CD14 |
10 |
GSM595796 | 1003 CD19 |
Week 2 |
CD19 |
10 |
GSM595797 | 1003 CD56 |
Week 2 |
CD56 |
10 |
GSM595798 | 1003 |
Week 2 |
Whole Blood |
10 |
GSM595799 | 1004 |
Week 4 |
Whole Blood |
10 |
GSM595800 | 1005 |
Week 8 |
Whole Blood |
10 |
GSM595801 | 1005_4 |
Week 8 |
CD4 |
10 |
GSM595802 | 1006 |
Week 12 |
Whole Blood |
10 |
GSM595803 | 1101 |
Pre-Tx |
Whole Blood |
11 |
GSM595804 | 1102 |
Week 1 |
Whole Blood |
11 |
GSM595805 | 1103 CD14 |
Week 2 |
CD14 |
11 |
GSM595806 | 1103 CD8 |
Week 2 |
CD8 |
11 |
GSM595807 | 1103 |
Week 2 |
Whole Blood |
11 |
GSM595808 | 1104a |
Week 4 |
Whole Blood |
11 |
GSM595809 | 1104b |
Week 4 |
Whole Blood |
11 |
GSM595810 | 1104_14 |
Week 4 |
CD14 |
11 |
GSM595811 | 1104_PBL |
Week 4 |
Whole blood |
11 |
GSM595812 | 1105_14 |
Week 8 |
CD14 |
11 |
GSM595813 | 1106 CD14 |
Week 12 |
CD14 |
11 |
GSM595814 | 1106 CD4 |
Week 12 |
CD4 |
11 |
GSM595815 | 1106 |
Week 12 |
Whole Blood |
11 |
GSM595816 | 1201 |
Pre-Tx |
Whole Blood |
12 |
GSM595817 | 1201_19 |
Pre-Tx |
CD19 |
12 |
GSM595818 | 1201_4 |
Pre-Tx |
CD4 |
12 |
GSM595819 | 1201_8 |
Pre-Tx |
CD8 |
12 |
GSM595820 | 1202 |
Week 1 |
Whole Blood |
12 |
GSM595821 | 1203 CD14 |
Week 2 |
CD14 |
12 |
GSM595822 | 1203 CD19 |
Week 2 |
CD19 |
12 |
GSM595823 | 1203 CD4 |
Week 2 |
CD4 |
12 |
GSM595824 | 1203 CD56 |
Week 2 |
CD56 |
12 |
GSM595825 | 1203 CD8 |
Week 2 |
CD8 |
12 |
GSM595826 | 1203 |
Week 2 |
Whole Blood |
12 |
GSM595827 | 1204 |
Week 4 |
Whole Blood |
12 |
GSM595828 | 1204_14 |
Week 4 |
CD14 |
12 |
GSM595829 | 1204_4 |
Week 4 |
CD4 |
12 |
GSM595830 | 1204_PBL |
Week 4 |
Whole blood |
12 |
GSM595831 | 1205 |
Week 8 |
Whole Blood |
12 |
GSM595832 | 1206 CD14 |
Week 12 |
CD14 |
12 |
GSM595833 | 1206 CD19 |
Week 12 |
CD19 |
12 |
GSM595834 | 1206 CD4 |
Week 12 |
CD4 |
12 |
GSM595835 | 1206 CD56 |
Week 12 |
CD56 |
12 |
GSM595836 | 1206 CD8 |
Week 12 |
CD8 |
12 |
GSM595837 | 1206-CD14_ |
Week 12 |
CD14 |
12 |
GSM595838 | 1206-CD19_ |
Week 12 |
CD19 |
12 |
GSM595839 | 1206-CD4_ |
Week 12 |
CD4 |
12 |
GSM595840 | 1206-CD8_ |
Week 12 |
CD8 |
12 |
GSM595841 | 1206 |
Week 12 |
Whole Blood |
12 |
GSM595842 | 1301 |
Pre-Tx |
Whole Blood |
13 |
GSM595843 | 1301_8 |
Pre-Tx |
CD8 |
13 |
GSM595844 | 1301_CD4 |
Pre-Tx |
CD4 |
13 |
GSM595845 | 1301_PBL |
Pre-Tx |
Whole blood |
13 |
GSM595846 | 1302 |
Week 1 |
Whole Blood |
13 |
GSM595847 | 1303 |
Week 2 |
Whole Blood |
13 |
GSM595848 | 1304 |
Week 4 |
Whole Blood |
13 |
GSM595849 | 1305 |
Week 8 |
Whole Blood |
13 |
GSM595850 | 1306 CD14 |
Week 12 |
CD14 |
13 |
GSM595851 | 1855 |
Control Day 0 |
Whole Blood |
Control 1 |
GSM595852 | 2 CD14 |
Control Day 0 |
CD14 |
Control 2 |
GSM595853 | 2 CD19 |
Control Day 0 |
CD19 |
Control 2 |
GSM595854 | 2 CD4 |
Control Day 0 |
CD4 |
Control 2 |
GSM595855 | 2 CD56 |
Control Day 0 |
CD56 |
Control 2 |
GSM595856 | 2 CD8 |
Control Day 0 |
CD8 |
Control 2 |
GSM595857 | 3 CD14 |
Control Day 0 |
CD14 |
Control 3 |
GSM595858 | 3 CD19 |
Control Day 0 |
CD19 |
Control 3 |
GSM595859 | 3 CD4 |
Control Day 0 |
CD4 |
Control 3 |
GSM595860 | 3 CD56 |
Control Day 0 |
CD56 |
Control 3 |
GSM595861 | 3 CD8 |
Control Day 0 |
CD8 |
Control 3 |
GSM595862 | 4 CD14 |
Control Day 0 |
CD14 |
Control 4 |
GSM595863 | 4 CD19 |
Control Day 0 |
CD19 |
Control 4 |
GSM595864 | 4 CD4 |
Control Day 0 |
CD4 |
Control 4 |
GSM595865 | 4 CD8 |
Control Day 0 |
CD8 |
Control 4 |
GSM595866 | 4559 |
Control Day 0 |
Whole Blood |
Control 2 |
GSM595867 | 4651 |
Control Day 0 |
Whole Blood |
Control 3 |
GSM595868 | 4674 |
Control Day 0 |
Whole Blood |
Control 4 |
GSM595869 | 5 CD14 |
Control Day 0 |
CD14 |
Control 5 |
GSM595870 | 5 CD19 |
Control Day 0 |
CD19 |
Control 5 |
GSM595871 | 5 CD4 |
Control Day 0 |
CD4 |
Control 5 |
GSM595872 | 5 CD8 |
Control Day 0 |
CD8 |
Control 5 |
GSM595873 | 94 |
Control Day 0 |
Whole Blood |
Control 5 |
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