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Clinical

Human Dendritic Cell Subtype Gene Arrays-GSE11943

Purpose

To provide better insight into the biology of plasmacytoid DCs

Hypothesis

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Experimental Design

We isolated these cells from the peripheral blood of healthy donors in order to further characterize their gene expression. Using gene array technology we compared the genetic profiles of these cells to those of CD14+ monocytes isolated from the same donors.

Experimental Variables

4 replicates of BDCA cells and CD14 cells

Controls

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Methods

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Additional Information
  • Wrzesinski SH, Fisher JL, Ernstoff MS. Genetic profiles of plasmacytoid (BDCA-4 expressing) DC subtypes-clues to DC subtype function in vivo. Exp Hematol Oncol 2013 Mar 9;2(1):8. PMID: 23497451
  • Microarray
    Affymetrix HG-U133A
    8 Samples Loaded: 8
    Human (Homo sapiens)
    Dendritic Cells , Monocytes
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title unstimulated CD14 cells from same donor unstimulated plasmacytoid DCs Cell type
    GSM301723 BDCA-4 biologic rep1 True pDCs
    GSM301724 BDCA-4 biologic rep2 True pDCs
    GSM301725 BDCA-4 biologic rep3 True pDCs
    GSM301726 BDCA-4 biologic rep4 True pDCs
    GSM301727 CD14 biologic rep1 True Monocyte
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    File
    sampleset4000036_sampleannotations.csv
    Sample Set Spreadsheet
    Raw Signal
    Raw Signal
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    Samples Viewer / Editor

    All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

    Sample ID !Sample Title Unstimulated CD14 cells from same donor Unstimulated plasmacytoid DCs Cell type
    GSM301723
    BDCA-4 biologic rep1
     
    True
    pDCs
    GSM301724
    BDCA-4 biologic rep2
     
    True
    pDCs
    GSM301725
    BDCA-4 biologic rep3
     
    True
    pDCs
    GSM301726
    BDCA-4 biologic rep4
     
    True
    pDCs
    GSM301727
    CD14 biologic rep1
    True
     
    Monocyte
    GSM301728
    CD14 biologic rep2
    True
     
    Monocyte
    GSM301729
    CD14 biologic rep3
    True
     
    Monocyte
    GSM301730
    CD14 biologic rep4
    True
     
    Monocyte

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