Gene expression profiling in pediatric meningococcal sepsis reveals dynamic changes in NK-cell and cytotoxic molecules-GSE11755
Meningococcal sepsis remains an important cause of childhood morbidity and mortality. Largely due to logistic complexities of research in young children with acute life-threatening disease, very little is known regarding differential expression kinetics and molecular regulation of immune response genes in leukocyte subsets.
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In this prospective case-control study, six children with meningococcal sepsis were included. Blood was drawn at four time points (t=0, t=8, t=24 and t=72 h after admission to the paediatric intensive care unit). Blood was also collected from matched controls. Detailed immunophenotyping of leukocytes was performed; RNA isolated from whole blood, lymphocytes, monocytes, and granulocytes was used to perform Affymetrix micro-array gene expression analysis.No replicates were performed.Table 4. Results of between-subject analyses.Source Time (h) ca co/ca at t=TBlood t0: ca:2 3, co: a b c; t8: 2; t24 :ca:1 2 3; t72: ca:1 2.Lym t0: ca:2 3, co:a b c d;t8: ca:1 3 4;t24: ca:1 2 3 4 6; t72: ca: 1 2.Mono t0:ca: 1 3 4 6, co: a b d; t8: ca:1 2 3; t24:ca: 1 2 3; t72: ca:1.Numbers represent the patient identification numbers.Characters represent the individual controls.
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In this prospective case-control study, six children with meningococcal sepsis were included. Blood was drawn at four time points (t=0, t=8, t=24 and t=72 h after admission to the paediatric intensive care unit). Blood was also collected from matched controls. Detailed immunophenotyping of leukocytes was performed; RNA isolated from whole blood, lymphocytes, monocytes, and granulocytes was used to perform Affymetrix micro-array gene expression analysis.
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Sample ID | !Sample_title | T8 Patient 1 | T8 patient 2 | T0 Patient 2 | T8 patient 1 |
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GSM297760 | 002_P1_Lym_T8 | True | |||
GSM297761 | 003_P1_Lym_T24 | ||||
GSM297762 | 004_P1_Lym_T72 | ||||
GSM297763 | 005_P1_Mon_T0 | ||||
GSM297764 | 006_P1_Mon_T8 | True |
All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.
Sample ID | !Sample Title | T8 Patient 1 | T8 patient 2 | T0 Patient 2 | T8 patient 1 | T8 patient 3 | T72 patient1 | T72 patient 1 | T72 patient 2 | T24 Patient 2 | T24 patient 4 | T24 Patient 1 | T24 patient 3 | T24 patient 2 | T24 patient 1 | Controle c | Controle b | Controle a | T0 patient 2 | T0 patient 1 | T0 patient 4 | T0 patient 3 | T0 patient 6 | Controle d | Controle b | T24 patient 6 | Controle c | Grouping | Time point |
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GSM297760 | 002_P1_Lym_T8 |
True |
Lymphocyte 8 h |
8 |
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GSM297761 | 003_P1_Lym_T24 |
True |
Lymphocyte 24 h |
24 |
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GSM297762 | 004_P1_Lym_T72 |
True |
Lymphocyte 72 h |
72 |
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GSM297763 | 005_P1_Mon_T0 |
True |
Monocyte 0 h |
0 |
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GSM297764 | 006_P1_Mon_T8 |
True |
Monocyte 8 h |
8 |
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GSM297765 | 007_P1_Mon_T24 |
True |
Monocyte 24 h |
24 |
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GSM297766 | 008_P1_Mon_T72 |
True |
Monocyte 72 h |
72 |
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GSM297767 | 015_P1_Blo_T24 |
True |
Whole blood 24 h |
24 |
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GSM297768 | 016_P1_Blo_T72 |
True |
Whole blood 72 h |
72 |
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GSM297769 | 017_P2_Lym_T0 |
True |
Lymphocyte 0 h |
0 |
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GSM297770 | 019_P2_Lym_T24 |
True |
Lymphocyte 24 h |
24 |
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GSM297771 | 020_P2_Lym_T72 |
True |
Lymphocyte 72 h |
72 |
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GSM297772 | 022_P2_Mon_T8 |
True |
Monocyte 8 h |
8 |
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GSM297773 | 023_P2_Mon_T24 |
True |
Monocyte 24 h |
24 |
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GSM297774 | 029_P2_Blo_T0 |
True |
Whole blood 0 h |
0 |
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GSM297775 | 030_P2_Blo_T8 |
True |
Whole blood 8 h |
8 |
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GSM297776 | 031_P2_Blo_T24 |
True |
Whole blood 24 h |
24 |
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GSM297777 | 032_P2_Blo_T72 |
True |
Whole blood 72 h |
72 |
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GSM297778 | 033_P3_Lym_T0 |
True |
Lymphocyte 0 h |
0 |
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GSM297779 | 034_P3_Lym_T8 |
True |
Lymphocyte 8 h |
8 |
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GSM297780 | 035_P3_Lym_T24 |
True |
Lymphocyte 24 h |
24 |
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GSM297781 | 036_P3_Mon_T0 |
True |
Monocyte 0 h |
0 |
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GSM297782 | 037_P3_Mon_T8 |
True |
Monocyte 8 h |
8 |
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GSM297783 | 038_P3_Mon_T24 |
True |
Monocyte 24 h |
24 |
|||||||||||||||||||||||||
GSM297784 | 042_P3_Blo_T0 |
True |
Whole blood 0 h |
0 |
|||||||||||||||||||||||||
GSM297785 | 044_P3_Blo_T24 |
True |
Whole blood 24 h |
24 |
|||||||||||||||||||||||||
GSM297786 | 046_P4_Lym_T8 |
True |
Lymphocyte 8 h |
8 |
|||||||||||||||||||||||||
GSM297787 | 047_P4_Lym_T24 |
True |
Lymphocyte 24 h |
24 |
|||||||||||||||||||||||||
GSM297788 | 048_P4_Mon_T0 |
True |
Monocyte 0 h |
0 |
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GSM297789 | 057_Cb_Lym |
True |
Lymphocyte control |
Control |
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GSM297790 | 058_Cb_Mon |
True |
Monocyte control |
Control |
|||||||||||||||||||||||||
GSM297791 | 060_Cb_Blo |
True |
Whole blood control |
Control |
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GSM297792 | 061_Cc_Lym |
True |
Lymphocyte control |
Control |
|||||||||||||||||||||||||
GSM297793 | 064_Cc_Blo |
True |
Whole blood control |
Control |
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GSM297794 | 065_Cd_Lym |
True |
Lymphocyte control |
Control |
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GSM297795 | 066_Cd_Mon |
True |
Monocyte control |
Control |
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GSM297796 | 069_Ca_Lym |
True |
Lymphocyte control |
Control |
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GSM297797 | 070_Ca_Mon |
True |
Monocyte control |
Control |
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GSM297798 | 072_Ca_Blo |
True |
Whole blood control |
Control |
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GSM297799 | 092_P6_Lym_T24 |
True |
Lymphocyte 24 h |
24 |
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GSM297800 | 093_P6_Mon_T0 |
True |
Monocyte 0 h |
0 |
Name | ||
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