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Clinical

Gene expression profiling in pediatric meningococcal sepsis reveals dynamic changes in NK-cell and cytotoxic molecules-GSE11755

Purpose

Meningococcal sepsis remains an important cause of childhood morbidity and mortality. Largely due to logistic complexities of research in young children with acute life-threatening disease, very little is known regarding differential expression kinetics and molecular regulation of immune response genes in leukocyte subsets.

Hypothesis

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Experimental Design

In this prospective case-control study, six children with meningococcal sepsis were included. Blood was drawn at four time points (t=0, t=8, t=24 and t=72 h after admission to the paediatric intensive care unit). Blood was also collected from matched controls. Detailed immunophenotyping of leukocytes was performed; RNA isolated from whole blood, lymphocytes, monocytes, and granulocytes was used to perform Affymetrix micro-array gene expression analysis.No replicates were performed.Table 4. Results of between-subject analyses.Source Time (h) ca co/ca at t=TBlood t0: ca:2 3, co: a b c; t8: 2; t24 :ca:1 2 3; t72: ca:1 2.Lym t0: ca:2 3, co:a b c d;t8: ca:1 3 4;t24: ca:1 2 3 4 6; t72: ca: 1 2.Mono t0:ca: 1 3 4 6, co: a b d; t8: ca:1 2 3; t24:ca: 1 2 3; t72: ca:1.Numbers represent the patient identification numbers.Characters represent the individual controls.

Experimental Variables

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Controls

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Methods

In this prospective case-control study, six children with meningococcal sepsis were included. Blood was drawn at four time points (t=0, t=8, t=24 and t=72 h after admission to the paediatric intensive care unit). Blood was also collected from matched controls. Detailed immunophenotyping of leukocytes was performed; RNA isolated from whole blood, lymphocytes, monocytes, and granulocytes was used to perform Affymetrix micro-array gene expression analysis.

Additional Information

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Microarray
Affymetrix HG-U133_Plus_2
41 Samples Loaded: 41
Human (Homo sapiens)
Lymphocytes , Whole Blood , Monocytes
Sepsis
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample_title T8 Patient 1 T8 patient 2 T0 Patient 2 T8 patient 1
GSM297760 002_P1_Lym_T8 True
GSM297761 003_P1_Lym_T24
GSM297762 004_P1_Lym_T72
GSM297763 005_P1_Mon_T0
GSM297764 006_P1_Mon_T8 True
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File
sampleset4000035_sampleannotations.csv
Sample Set Spreadsheet
Raw Signal
Raw Signal
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Clinical Datasource
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Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID !Sample Title T8 Patient 1 T8 patient 2 T0 Patient 2 T8 patient 1 T8 patient 3 T72 patient1 T72 patient 1 T72 patient 2 T24 Patient 2 T24 patient 4 T24 Patient 1 T24 patient 3 T24 patient 2 T24 patient 1 Controle c Controle b Controle a T0 patient 2 T0 patient 1 T0 patient 4 T0 patient 3 T0 patient 6 Controle d Controle b T24 patient 6 Controle c Grouping Time point
GSM297760
002_P1_Lym_T8
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Lymphocyte 8 h
8
GSM297761
003_P1_Lym_T24
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
Lymphocyte 24 h
24
GSM297762
004_P1_Lym_T72
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Lymphocyte 72 h
72
GSM297763
005_P1_Mon_T0
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
Monocyte 0 h
0
GSM297764
006_P1_Mon_T8
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Monocyte 8 h
8
GSM297765
007_P1_Mon_T24
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Monocyte 24 h
24
GSM297766
008_P1_Mon_T72
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Monocyte 72 h
72
GSM297767
015_P1_Blo_T24
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Whole blood 24 h
24
GSM297768
016_P1_Blo_T72
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Whole blood 72 h
72
GSM297769
017_P2_Lym_T0
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Lymphocyte 0 h
0
GSM297770
019_P2_Lym_T24
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Lymphocyte 24 h
24
GSM297771
020_P2_Lym_T72
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Lymphocyte 72 h
72
GSM297772
022_P2_Mon_T8
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Monocyte 8 h
8
GSM297773
023_P2_Mon_T24
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Monocyte 24 h
24
GSM297774
029_P2_Blo_T0
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
Whole blood 0 h
0
GSM297775
030_P2_Blo_T8
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Whole blood 8 h
8
GSM297776
031_P2_Blo_T24
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
Whole blood 24 h
24
GSM297777
032_P2_Blo_T72
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Whole blood 72 h
72
GSM297778
033_P3_Lym_T0
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
Lymphocyte 0 h
0
GSM297779
034_P3_Lym_T8
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Lymphocyte 8 h
8
GSM297780
035_P3_Lym_T24
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Lymphocyte 24 h
24
GSM297781
036_P3_Mon_T0
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
Monocyte 0 h
0
GSM297782
037_P3_Mon_T8
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Monocyte 8 h
8
GSM297783
038_P3_Mon_T24
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Monocyte 24 h
24
GSM297784
042_P3_Blo_T0
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
Whole blood 0 h
0
GSM297785
044_P3_Blo_T24
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Whole blood 24 h
24
GSM297786
046_P4_Lym_T8
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
Lymphocyte 8 h
8
GSM297787
047_P4_Lym_T24
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Lymphocyte 24 h
24
GSM297788
048_P4_Mon_T0
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
Monocyte 0 h
0
GSM297789
057_Cb_Lym
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
Lymphocyte control
Control
GSM297790
058_Cb_Mon
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
Monocyte control
Control
GSM297791
060_Cb_Blo
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
Whole blood control
Control
GSM297792
061_Cc_Lym
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
 
 
Lymphocyte control
Control
GSM297793
064_Cc_Blo
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
Whole blood control
Control
GSM297794
065_Cd_Lym
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
Lymphocyte control
Control
GSM297795
066_Cd_Mon
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
Monocyte control
Control
GSM297796
069_Ca_Lym
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
Lymphocyte control
Control
GSM297797
070_Ca_Mon
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
Monocyte control
Control
GSM297798
072_Ca_Blo
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
 
 
 
 
 
Whole blood control
Control
GSM297799
092_P6_Lym_T24
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
Lymphocyte 24 h
24
GSM297800
093_P6_Mon_T0
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
True
 
 
 
 
Monocyte 0 h
0

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