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Clinical

Gene expression of Peg-interferon alfa-2b plus ribavirin in hepatitis C patients during the first 10 weeks of treatment-GSE11342

Purpose

To study the kinetics of gene expression change in PBMC of HCV patients treated with Peg-IFN-alpha2 and ribavirin.

Hypothesis

Study of PBMC gene expression during the first 10 weeks of therapy with Pegylated-interferon-alfa2b (PegIntronTM) and ribavirin (administered by weight) in HCV patients.

Experimental Design

RNA was isolated from PBMC from 20 treatment-naïve patients just before treatment (day 0,) and at days 3, 6, 10, 13, 27, 42 and 70 days after treatment. Gene expression at each time was compared to that of day 0 using Affymetrix DNA-micro-arrays.

Experimental Variables

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Controls

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Methods

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Additional Information

Focusing upon genes that changed at least 1.5-fold and p<0.001, the numbers were high at day 3 (300 probes) and 10 (255) but dropped at days 6 (128) and 13 (142) but were steady at later time points. A large number of genes continued to be up regulated throughout the trial period. A second group of genes, including CXCL10, chemokine receptor 1, TRAIL, IL1 R and genes associated with complement and lipid metabolism, were transiently induced. CDKN1C (cyclin kinase inhibitor 1) was induced early but repressed at later times. Genes induced at later times were mostly related to blood chemistry and oxygen transport. Genes down regulated were related to ribosomal proteins and eukaryotic translation complexes. By week 10, 50% of the patients demonstrated a positive response to therapy. Conclusions: The response to pegintron/ribavirin was similar to that reported for Pegasys/ribavirin, although the extent of gene induction was less. No differences were found between patients responding to treatment or non-responders during this period. Our data suggest that more than once a week dosing might be desirable early on during treatment.

Microarray
Affymetrix HG-U133A
152 Samples Loaded: 152
Human (Homo sapiens)
PBMC
HCV
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample_title Patient id Caucasian African American Female
GSM286439 Patient_1_day_0 1 True
GSM286440 Patient_1_day_3 1 True
GSM286441 Patient_1_day_6 1 True
GSM286442 Patient_1_day_10 1 True
GSM286443 Patient_1_day_27 1 True
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All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID !Sample Title Patient id Caucasian African American Female Male Time Point (day)
GSM286439
Patient_1_day_0
1
True
 
 
True
0
GSM286440
Patient_1_day_3
1
True
 
 
True
3
GSM286441
Patient_1_day_6
1
True
 
 
True
6
GSM286442
Patient_1_day_10
1
True
 
 
True
10
GSM286443
Patient_1_day_27
1
True
 
 
True
27
GSM286444
Patient_1_day_42
1
True
 
 
True
42
GSM286445
Patient_1_day_70
1
True
 
 
True
70
GSM286446
Patient_2_day_0
2
True
 
True
 
0
GSM286447
Patient_2_day_3
2
True
 
True
 
3
GSM286448
Patient_2_day_6
2
True
 
True
 
6
GSM286449
Patient_2_day_10
2
True
 
True
 
10
GSM286450
Patient_2_day_27
2
True
 
True
 
27
GSM286451
Patient_2_day_42
2
True
 
True
 
42
GSM286452
Patient_2_day_70
2
True
 
True
 
70
GSM286453
Patient_3_day_0
3
True
 
 
True
0
GSM286454
Patient_3_day_3
3
True
 
 
True
3
GSM286455
Patient_3_day_6
3
True
 
 
True
6
GSM286456
Patient_3_day_10
3
True
 
 
True
10
GSM286457
Patient_3_day_27
3
True
 
 
True
27
GSM286458
Patient_3_day_42
3
True
 
 
True
42
GSM286459
Patient_3_day_70
3
True
 
 
True
70
GSM286460
Patient_4_day_0
4
True
 
 
True
0
GSM286461
Patient_4_day_3
4
True
 
 
True
3
GSM286462
Patient_4_day_13
4
True
 
 
True
13
GSM286463
Patient_4_day_27
4
True
 
 
True
27
GSM286464
Patient_4_day_42
4
True
 
 
True
42
GSM286465
Patient_4_day_70
4
True
 
 
True
70
GSM286466
Patient_5_day_0
5
True
 
 
True
0
GSM286467
Patient_5_day_3
5
True
 
 
True
3
GSM286468
Patient_5_day_10
5
True
 
 
True
10
GSM286469
Patient_5_day_13
5
True
 
 
True
13
GSM286470
Patient_5_day_27
5
True
 
 
True
27
GSM286471
Patient_5_day_42
5
True
 
 
True
42
GSM286472
Patient_5_day_70
5
True
 
 
True
70
GSM286473
Patient_6_day_0
6
True
 
 
True
0
GSM286474
Patient_6_day_3
6
True
 
 
True
3
GSM286475
Patient_6_day_10
6
True
 
 
True
10
GSM286476
Patient_6_day_13
6
True
 
 
True
13
GSM286477
Patient_6_day_27
6
True
 
 
True
27
GSM286478
Patient_6_day_42
6
True
 
 
True
42
GSM286479
Patient_6_day_70
6
True
 
 
True
70
GSM286480
Patient_7_day_0
7
True
 
 
True
0
GSM286481
Patient_7_day_3
7
True
 
 
True
3
GSM286482
Patient_7_day_6
7
True
 
 
True
6
GSM286483
Patient_7_day_10
7
True
 
 
True
10
GSM286484
Patient_7_day_13
7
True
 
 
True
13
GSM286485
Patient_7_day_27
7
True
 
 
True
27
GSM286486
Patient_7_day_42
7
True
 
 
True
42
GSM286487
Patient_7_day_70
7
True
 
 
True
70
GSM286488
Patient_8_day_0
8
True
 
 
True
0
GSM286489
Patient_8_day_3
8
True
 
 
True
3
GSM286490
Patient_8_day_6
8
True
 
 
True
6
GSM286491
Patient_8_day_10
8
True
 
 
True
10
GSM286492
Patient_8_day_13
8
True
 
 
True
13
GSM286493
Patient_8_day_27
8
True
 
 
True
27
GSM286494
Patient_8_day_42
8
True
 
 
True
42
GSM286495
Patient_8_day_70
8
True
 
 
True
70
GSM286496
Patient_9_day_0
9
True
 
 
True
0
GSM286497
Patient_9_day_3
9
True
 
 
True
3
GSM286498
Patient_9_day_6
9
True
 
 
True
6
GSM286499
Patient_9_day_10
9
True
 
 
True
10
GSM286500
Patient_9_day_13
9
True
 
 
True
13
GSM286501
Patient_9_day_27
9
True
 
 
True
27
GSM286502
Patient_9_day_42
9
True
 
 
True
42
GSM286503
Patient_9_day_70
9
True
 
 
True
70
GSM286504
Patient_10_day_0
10
True
 
 
True
0
GSM286505
Patient_10_day_3
10
True
 
 
True
3
GSM286506
Patient_10_day_6
10
True
 
 
True
6
GSM286507
Patient_10_day_10
10
True
 
 
True
10
GSM286508
Patient_10_day_13
10
True
 
 
True
13
GSM286509
Patient_10_day_27
10
True
 
 
True
27
GSM286510
Patient_10_day_42
10
True
 
 
True
42
GSM286511
Patient_10_day_70
10
True
 
 
True
70
GSM286512
Patient_11_day_0
11
True
 
True
 
0
GSM286513
Patient_11_day_6
11
True
 
True
 
6
GSM286514
Patient_11_day_10
11
True
 
True
 
10
GSM286515
Patient_11_day_13
11
True
 
True
 
13
GSM286516
Patient_11_day_27
11
True
 
True
 
27
GSM286517
Patient_11_day_42
11
True
 
True
 
42
GSM286518
Patient_11_day_70
11
True
 
True
 
70
GSM286519
Patient_12_day_0
12
 
True
True
 
0
GSM286520
Patient_12_day_3
12
 
True
True
 
3
GSM286521
Patient_12_day_6
12
 
True
True
 
6
GSM286522
Patient_12_day_10
12
 
True
True
 
10
GSM286523
Patient_12_day_13
12
 
True
True
 
13
GSM286524
Patient_12_day_27
12
 
True
True
 
27
GSM286525
Patient_12_day_42
12
 
True
True
 
42
GSM286526
Patient_12_day_70
12
 
True
True
 
70
GSM286527
Patient_13_day_0
13
True
 
 
True
0
GSM286528
Patient_13_day_3
13
True
 
 
True
3
GSM286529
Patient_13_day_6
13
True
 
 
True
6
GSM286530
Patient_13_day_10
13
True
 
 
True
10
GSM286531
Patient_13_day_13
13
True
 
 
True
13
GSM286532
Patient_13_day_27
13
True
 
 
True
27
GSM286533
Patient_13_day_42
13
True
 
 
True
42
GSM286534
Patient_13_day_70
13
True
 
 
True
70
GSM286535
Patient_14_day_0
14
True
 
 
True
0
GSM286536
Patient_14_day_3
14
True
 
 
True
3
GSM286537
Patient_14_day_6
14
True
 
 
True
6
GSM286538
Patient_14_day_10
14
True
 
 
True
10
GSM286539
Patient_14_day_13
14
True
 
 
True
13
GSM286540
Patient_14_day_27
14
True
 
 
True
27
GSM286541
Patient_14_day_42
14
True
 
 
True
42
GSM286542
Patient_14_day_70
14
True
 
 
True
70
GSM286543
Patient_15_day_0
15
True
 
True
 
0
GSM286544
Patient_15_day_3
15
True
 
True
 
3
GSM286545
Patient_15_day_6
15
True
 
True
 
6
GSM286546
Patient_15_day_10
15
True
 
True
 
10
GSM286547
Patient_15_day_13
15
True
 
True
 
13
GSM286548
Patient_15_day_27
15
True
 
True
 
27
GSM286549
Patient_15_day_42
15
True
 
True
 
42
GSM286550
Patient_15_day_70
15
True
 
True
 
70
GSM286551
Patient_16_day_0
16
True
 
 
True
0
GSM286552
Patient_16_day_3
16
True
 
 
True
3
GSM286553
Patient_16_day_6
16
True
 
 
True
6
GSM286554
Patient_16_day_10
16
True
 
 
True
10
GSM286555
Patient_16_day_13
16
True
 
 
True
13
GSM286556
Patient_16_day_27
16
True
 
 
True
27
GSM286557
Patient_16_day_42
16
True
 
 
True
42
GSM286558
Patient_16_day_70
16
True
 
 
True
70
GSM286559
Patient_17_day_0
17
True
 
 
True
0
GSM286560
Patient_17_day_3
17
True
 
 
True
3
GSM286561
Patient_17_day_6
17
True
 
 
True
6
GSM286562
Patient_17_day_10
17
True
 
 
True
10
GSM286563
Patient_17_day_13
17
True
 
 
True
13
GSM286564
Patient_17_day_27
17
True
 
 
True
27
GSM286565
Patient_17_day_42
17
True
 
 
True
42
GSM286566
Patient_17_day_70
17
True
 
 
True
70
GSM286567
Patient_18_day_0
18
True
 
 
True
0
GSM286568
Patient_18_day_3
18
True
 
 
True
3
GSM286569
Patient_18_day_6
18
True
 
 
True
6
GSM286570
Patient_18_day_10
18
True
 
 
True
10
GSM286571
Patient_18_day_13
18
True
 
 
True
13
GSM286572
Patient_18_day_27
18
True
 
 
True
27
GSM286573
Patient_18_day_42
18
True
 
 
True
42
GSM286574
Patient_18_day_70
18
True
 
 
True
70
GSM286575
Patient_19_day_0
19
True
 
 
True
0
GSM286576
Patient_19_day_3
19
True
 
 
True
3
GSM286577
Patient_19_day_6
19
True
 
 
True
6
GSM286578
Patient_19_day_10
19
True
 
 
True
10
GSM286579
Patient_19_day_13
19
True
 
 
True
13
GSM286580
Patient_19_day_27
19
True
 
 
True
27
GSM286581
Patient_19_day_42
19
True
 
 
True
42
GSM286582
Patient_19_day_70
19
True
 
 
True
70
GSM286583
Patient_20_day_0
20
True
 
 
True
0
GSM286584
Patient_20_day_3
20
True
 
 
True
3
GSM286585
Patient_20_day_6
20
True
 
 
True
6
GSM286586
Patient_20_day_10
20
True
 
 
True
10
GSM286587
Patient_20_day_13
20
True
 
 
True
13
GSM286588
Patient_20_day_27
20
True
 
 
True
27
GSM286589
Patient_20_day_42
20
True
 
 
True
42
GSM286590
Patient_20_day_70
20
True
 
 
True
70

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