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Clinical

PGE2-induced OSM expression-GSE36995

Purpose

The objective of the study was to compare the wound macs with corresponding macs derived from peripheral blood monocytes (MDMs). Wound site macrophage (wound macs were isolated from human subjects with chronic wounds. Matching blood monocyte derived macrophages (MDM) were obtained from same subjects. Transcriptome profiling (GeneChip, Affymetrix) was performed.The expression values of genes were normalized using global scaling approach.

Hypothesis

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Experimental Design

Blood monocyte derived macrophages or human wound macrophages were isolated and transcriptome analysis was performed using affymetrix gene chip analysis.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Ganesh K, Das A, Dickerson R, Khanna S et al. Prostaglandin E? induces oncostatin M expression in human chronic wound macrophages through Axl receptor tyrosine kinase pathway. J Immunol 2012 Sep 1;189(5):2563-73. PMID: 22844123
  • Microarray
    Affymetrix HG-U133_Plus_2
    6 Samples Loaded: 6
    Human (Homo sapiens)
    Monocyte-derived macrophages (MDMs) , Macrophages
    Inflammation
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title cell type tissue source
    GSM907880 mac-1 blood monocyte derived macrophage peripheral blood monocytes
    GSM907974 mac-2 blood monocyte derived macrophage peripheral blood monocytes
    GSM907975 mac-3 blood monocyte derived macrophage peripheral blood monocytes
    GSM908231 WMAC-1 wound macrophages human subjects with chronic wounds
    GSM908232 WMAC-2 wound macrophages human subjects with chronic wounds
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    Log2 Transformed
    Raw Signal
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    Sample ID !Sample Title Cell type Tissue source
    GSM907880
    mac-1
    blood monocyte derived macrophage
    peripheral blood monocytes
    GSM907974
    mac-2
    blood monocyte derived macrophage
    peripheral blood monocytes
    GSM907975
    mac-3
    blood monocyte derived macrophage
    peripheral blood monocytes
    GSM908231
    WMAC-1
    wound macrophages
    human subjects with chronic wounds
    GSM908232
    WMAC-2
    wound macrophages
    human subjects with chronic wounds
    GSM908233
    WMAC-3
    wound macrophages
    human subjects with chronic wounds

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