Sample Set Annotation Tool: PGE2-induced OSM expression-GSE36995

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

The objective of the study was to compare the wound macs with corresponding macs derived from peripheral blood monocytes (MDMs). Wound site macrophage (wound macs were isolated from human subjects with chronic wounds. Matching blood monocyte derived macrophages (MDM) were obtained from same subjects. Transcriptome profiling (GeneChip, Affymetrix) was performed.The expression values of genes were normalized using global scaling approach.

Hypothesis

Experimental Design

Blood monocyte derived macrophages or human wound macrophages were isolated and transcriptome analysis was performed using affymetrix gene chip analysis.

Experimental Variables

Controls

Methods

Additional Information

Ganesh K, Das A, Dickerson R, Khanna S et al. Prostaglandin E? induces oncostatin M expression in human chronic wound macrophages through Axl receptor tyrosine kinase pathway. J Immunol 2012 Sep 1;189(5):2563-73. PMID: 22844123

Expected Samples:

6 Samples Loaded: 6

Species

Human (Homo sapiens)

Sample Source

Monocyte-derived macrophages (MDMs) , Macrophages

Disease

Inflammation

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	cell type	tissue source
GSM907880	mac-1	blood monocyte derived macrophage	peripheral blood monocytes
GSM907974	mac-2	blood monocyte derived macrophage	peripheral blood monocytes
GSM907975	mac-3	blood monocyte derived macrophage	peripheral blood monocytes
GSM908231	WMAC-1	wound macrophages	human subjects with chronic wounds
GSM908232	WMAC-2	wound macrophages	human subjects with chronic wounds

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Sample ID	!Sample Title	Cell type	Tissue source
GSM907880	mac-1	blood monocyte derived macrophage	peripheral blood monocytes
GSM907974	mac-2	blood monocyte derived macrophage	peripheral blood monocytes
GSM907975	mac-3	blood monocyte derived macrophage	peripheral blood monocytes
GSM908231	WMAC-1	wound macrophages	human subjects with chronic wounds
GSM908232	WMAC-2	wound macrophages	human subjects with chronic wounds
GSM908233	WMAC-3	wound macrophages	human subjects with chronic wounds

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Module Analysis

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SIDRA