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Clinical

Nanotoxicogenomic study of ZnO and TiO2 responses-GSE39316

Purpose

To identify signaling pathways underlying the cytotoxicity caused by ZnO nanoparticles.

Hypothesis

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Experimental Design

Altogether 90 samples were analyzed originating from three biological replicates of HMDM, MDDC or Jurkat cells containg untreated control cells, ZnO or TiO2 treated cells with doses 1µg/ml and 10µg/ml. The sample timepoints were 6 hours and 24 hours.

Experimental Variables

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Controls

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Methods

HMDM and Jurkat cells were seeded in 24-well plates at a density of 1×106 cells/well and 1.5×105 cells/well respectively in a final volume of 1 ml and treated with the indicated concentrations of nanoparticles for the indicated times [7]. Immature MDDC were seeded in 6-well plates at a density of 4×105 cells/ml in a final volume of 3 ml [23]. Nanoparticle exposures were done in the culturing mediums without any cytokines.

Additional Information
  • Tuomela S, Autio R, Buerki-Thurnherr T, Arslan O et al. Gene expression profiling of immune-competent human cells exposed to engineered zinc oxide or titanium dioxide nanoparticles. PLoS One 2013;8(7):e68415. PMID: 23894303
  • Microarray
    Illumina HumanHT-12 v3
    90 Samples Loaded: 90
    Human (Homo sapiens)
    Monocyte-derived Dendritic cells (MDCs) , Monocyte-derived macrophages (MDMs)
    Healthy
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title timepoint treatment cell type
    GSM960767 MDDC_rep1_ctr_6h_0 6h untreated MDDC
    GSM960768 MDDC_rep1_ctr_24h_0 24h untreated MDDC
    GSM960769 MDDC_rep1_TiO2_6h_1 6h TiO2 1µg/ml MDDC
    GSM960770 MDDC_rep1_TiO2_6h_10 6h TiO2 10µg/ml MDDC
    GSM960771 MDDC_rep1_TiO2_24h_1 24h TiO2 1µg/ml MDDC
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    File
    Log2 Transformed
    Raw Signal
    •   |  View in GXB »
    Clinical Datasource
    Links

    Samples Viewer / Editor

    All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

    Sample ID !Sample Title Timepoint Treatment Cell type
    GSM960767
    MDDC_rep1_ctr_6h_0
    6h
    untreated
    MDDC
    GSM960768
    MDDC_rep1_ctr_24h_0
    24h
    untreated
    MDDC
    GSM960769
    MDDC_rep1_TiO2_6h_1
    6h
    TiO2 1µg/ml
    MDDC
    GSM960770
    MDDC_rep1_TiO2_6h_10
    6h
    TiO2 10µg/ml
    MDDC
    GSM960771
    MDDC_rep1_TiO2_24h_1
    24h
    TiO2 1µg/ml
    MDDC
    GSM960772
    MDDC_rep1_TiO2_24h_10
    24h
    TiO2 10µg/ml
    MDDC
    GSM960773
    MDDC_rep1_ZnO_6h_1
    6h
    ZnO 1µg/ml
    MDDC
    GSM960774
    MDDC_rep1_ZnO_6h_10
    6h
    ZnO 10µg/ml
    MDDC
    GSM960775
    MDDC_rep1_ZnO_24h_1
    24h
    ZnO 1µg/ml
    MDDC
    GSM960776
    MDDC_rep1_ZnO_24h_10
    24h
    ZnO 10µg/ml
    MDDC
    GSM960777
    MDDC_rep2_ctr_6h_0
    6h
    untreated
    MDDC
    GSM960778
    MDDC_rep2_ctr_24h_0
    24h
    untreated
    MDDC
    GSM960779
    MDDC_rep2_TiO2_6h_1
    6h
    TiO2 1µg/ml
    MDDC
    GSM960780
    MDDC_rep2_TiO2_6h_10
    6h
    TiO2 10µg/ml
    MDDC
    GSM960781
    MDDC_rep2_TiO2_24h_1
    24h
    TiO2 1µg/ml
    MDDC
    GSM960782
    MDDC_rep2_TiO2_24h_10
    24h
    TiO2 10µg/ml
    MDDC
    GSM960783
    MDDC_rep2_ZnO_6h_1
    6h
    ZnO 1µg/ml
    MDDC
    GSM960784
    MDDC_rep2_ZnO_6h_10
    6h
    ZnO 10µg/ml
    MDDC
    GSM960785
    MDDC_rep2_ZnO_24h_1
    24h
    ZnO 1µg/ml
    MDDC
    GSM960786
    MDDC_rep2_ZnO_24h_10
    24h
    ZnO 10µg/ml
    MDDC
    GSM960787
    MDDC_rep3_ctr_6h_0
    6h
    untreated
    MDDC
    GSM960788
    MDDC_rep3_ctr_24h_0
    24h
    untreated
    MDDC
    GSM960789
    MDDC_rep3_TiO2_6h_1
    6h
    TiO2 1µg/ml
    MDDC
    GSM960790
    MDDC_rep3_TiO2_6h_10
    6h
    TiO2 10µg/ml
    MDDC
    GSM960791
    MDDC_rep3_TiO2_24h_1
    24h
    TiO2 1µg/ml
    MDDC
    GSM960792
    MDDC_rep3_TiO2_24h_10
    24h
    TiO2 10µg/ml
    MDDC
    GSM960793
    MDDC_rep3_ZnO_6h_1
    6h
    ZnO 1µg/ml
    MDDC
    GSM960794
    MDDC_rep3_ZnO_6h_10
    6h
    ZnO 10µg/ml
    MDDC
    GSM960795
    MDDC_rep3_ZnO_24h_1
    24h
    ZnO 1µg/ml
    MDDC
    GSM960796
    MDDC_rep3_ZnO_24h_10
    24h
    ZnO 10µg/ml
    MDDC
    GSM960797
    HMDC_rep1_ctr_6h_0
    6h
    untreated
    HMDM
    GSM960798
    HMDC_rep1_ctr_24h_0
    24h
    untreated
    HMDM
    GSM960799
    HMDC_rep1_TiO2_6h_1
    6h
    TiO2 1µg/ml
    HMDM
    GSM960800
    HMDC_rep1_TiO2_6h_10
    6h
    TiO2 10µg/ml
    HMDM
    GSM960801
    HMDC_rep1_TiO2_24h_1
    24h
    TiO2 1µg/ml
    HMDM
    GSM960802
    HMDC_rep1_TiO2_24h_10
    24h
    TiO2 10µg/ml
    HMDM
    GSM960803
    HMDC_rep1_ZnO_6h_1
    6h
    ZnO 1µg/ml
    HMDM
    GSM960804
    HMDC_rep1_ZnO_6h_10
    6h
    ZnO 10µg/ml
    HMDM
    GSM960805
    HMDC_rep1_ZnO_24h_1
    24h
    ZnO 1µg/ml
    HMDM
    GSM960806
    HMDC_rep1_ZnO_24h_10
    24h
    ZnO 10µg/ml
    HMDM
    GSM960807
    HMDC_rep2_ctr_6h_0
    6h
    untreated
    HMDM
    GSM960808
    HMDC_rep2_ctr_24h_0
    24h
    untreated
    HMDM
    GSM960809
    HMDC_rep2_TiO2_6h_1
    6h
    TiO2 1µg/ml
    HMDM
    GSM960810
    HMDC_rep2_TiO2_6h_10
    6h
    TiO2 10µg/ml
    HMDM
    GSM960811
    HMDC_rep2_TiO2_24h_1
    24h
    TiO2 1µg/ml
    HMDM
    GSM960812
    HMDC_rep2_TiO2_24h_10
    24h
    TiO2 10µg/ml
    HMDM
    GSM960813
    HMDC_rep2_ZnO_6h_1
    6h
    ZnO 1µg/ml
    HMDM
    GSM960814
    HMDC_rep2_ZnO_6h_10
    6h
    ZnO 10µg/ml
    HMDM
    GSM960815
    HMDC_rep2_ZnO_24h_1
    24h
    ZnO 1µg/ml
    HMDM
    GSM960816
    HMDC_rep2_ZnO_24h_10
    24h
    ZnO 10µg/ml
    HMDM
    GSM960817
    HMDC_rep3_ctr_6h_0
    6h
    untreated
    HMDM
    GSM960818
    HMDC_rep3_ctr_24h_0
    24h
    untreated
    HMDM
    GSM960819
    HMDC_rep3_TiO2_6h_1
    6h
    TiO2 1µg/ml
    HMDM
    GSM960820
    HMDC_rep3_TiO2_6h_10
    6h
    TiO2 10µg/ml
    HMDM
    GSM960821
    HMDC_rep3_TiO2_24h_1
    24h
    TiO2 1µg/ml
    HMDM
    GSM960822
    HMDC_rep3_TiO2_24h_10
    24h
    TiO2 10µg/ml
    HMDM
    GSM960823
    HMDC_rep3_ZnO_6h_1
    6h
    ZnO 1µg/ml
    HMDM
    GSM960824
    HMDC_rep3_ZnO_6h_10
    6h
    ZnO 10µg/ml
    HMDM
    GSM960825
    HMDC_rep3_ZnO_24h_1
    24h
    ZnO 1µg/ml
    HMDM
    GSM960826
    HMDC_rep3_ZnO_24h_10
    24h
    ZnO 10µg/ml
    HMDM
    GSM960827
    Jurkat_rep1_ctr_6h_0
    6h
    untreated
    Jurkat
    GSM960828
    Jurkat_rep1_ctr_24h_0
    24h
    untreated
    Jurkat
    GSM960829
    Jurkat_rep1_TiO2_6h_1
    6h
    TiO2 1µg/ml
    Jurkat
    GSM960830
    Jurkat_rep1_TiO2_6h_10
    6h
    TiO2 10µg/ml
    Jurkat
    GSM960831
    Jurkat_rep1_TiO2_24h_1
    24h
    TiO2 1µg/ml
    Jurkat
    GSM960832
    Jurkat_rep1_TiO2_24h_10
    24h
    TiO2 10µg/ml
    Jurkat
    GSM960833
    Jurkat_rep1_ZnO_6h_1
    6h
    ZnO 1µg/ml
    Jurkat
    GSM960834
    Jurkat_rep1_ZnO_6h_10
    6h
    ZnO 10µg/ml
    Jurkat
    GSM960835
    Jurkat_rep1_ZnO_24h_1
    24h
    ZnO 1µg/ml
    Jurkat
    GSM960836
    Jurkat_rep1_ZnO_24h_10
    24h
    ZnO 10µg/ml
    Jurkat
    GSM960837
    Jurkat_rep2_ctr_6h_0
    6h
    untreated
    Jurkat
    GSM960838
    Jurkat_rep2_ctr_24h_0
    24h
    untreated
    Jurkat
    GSM960839
    Jurkat_rep2_TiO2_6h_1
    6h
    TiO2 1µg/ml
    Jurkat
    GSM960840
    Jurkat_rep2_TiO2_6h_10
    6h
    TiO2 10µg/ml
    Jurkat
    GSM960841
    Jurkat_rep2_TiO2_24h_1
    24h
    TiO2 1µg/ml
    Jurkat
    GSM960842
    Jurkat_rep2_TiO2_24h_10
    24h
    TiO2 10µg/ml
    Jurkat
    GSM960843
    Jurkat_rep2_ZnO_6h_1
    6h
    ZnO 1µg/ml
    Jurkat
    GSM960844
    Jurkat_rep2_ZnO_6h_10
    6h
    ZnO 10µg/ml
    Jurkat
    GSM960845
    Jurkat_rep2_ZnO_24h_1
    24h
    ZnO 1µg/ml
    Jurkat
    GSM960846
    Jurkat_rep2_ZnO_24h_10
    24h
    ZnO 10µg/ml
    Jurkat
    GSM960847
    Jurkat_rep3_ctr_6h_0
    6h
    untreated
    Jurkat
    GSM960848
    Jurkat_rep3_ctr_24h_0
    24h
    untreated
    Jurkat
    GSM960849
    Jurkat_rep3_TiO2_6h_1
    6h
    TiO2 1µg/ml
    Jurkat
    GSM960850
    Jurkat_rep3_TiO2_6h_10
    6h
    TiO2 10µg/ml
    Jurkat
    GSM960851
    Jurkat_rep3_TiO2_24h_1
    24h
    TiO2 1µg/ml
    Jurkat
    GSM960852
    Jurkat_rep3_TiO2_24h_10
    24h
    TiO2 10µg/ml
    Jurkat
    GSM960853
    Jurkat_rep3_ZnO_6h_1
    6h
    ZnO 1µg/ml
    Jurkat
    GSM960854
    Jurkat_rep3_ZnO_6h_10
    6h
    ZnO 10µg/ml
    Jurkat
    GSM960855
    Jurkat_rep3_ZnO_24h_1
    24h
    ZnO 1µg/ml
    Jurkat
    GSM960856
    Jurkat_rep3_ZnO_24h_10
    24h
    ZnO 10µg/ml
    Jurkat

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