Microarray analysis of IL-10 stimulated adherent peripheral blood mononuclear cells-GSE43700
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Peripheral blood monuclear cells derived through Ficoll-Hypaque from the blood of healthy human donors (n=4). Cells adhered to tissue culture treated 6-well plates for 2 hours in 1% Fetal Bovine Serum (FBS) RPMI and then stimulated by IL-10 (R&D Systems) 10ng/ml or media alone in 10% FBS RPMI for 24 hours at 37°C, 5% CO2. After stimulation, cells harvested and monocytes isolated through CD14 positive selection (Miltenyi Biotec). RNA from purified monocytes extracted by Trizol and purified by Qiagen RNeasy Kit. RNA probe and microarray performed by UCLA Clinical Microarray Core using Ambion labeling kit and Affymetrix Human U133 Plus 2.0 array.
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Sample ID | Donor ID | !Sample_title | cell type | stimulated with |
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GSM1068613 | 1 | A6_media | Peripheral blood monuclear cells | media alone for 24hr |
GSM1068614 | 1 | A7_IL-10 | Peripheral blood monuclear cells | IL-10 (10ng/ml) for 24hr |
GSM1068615 | 2 | B6_media | Peripheral blood monuclear cells | media alone for 24hr |
GSM1068616 | 2 | B7_IL-10 | Peripheral blood monuclear cells | IL-10 (10ng/ml) for 24hr |
GSM1068617 | 3 | C6_media | Peripheral blood monuclear cells | media alone for 24hr |
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Sample ID | Donor ID | !Sample Title | Cell type | Stimulated with |
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GSM1068613 | 1 |
A6_media |
Peripheral blood monuclear cells |
media alone for 24hr |
GSM1068614 | 1 |
A7_IL-10 |
Peripheral blood monuclear cells |
IL-10 (10ng/ml) for 24hr |
GSM1068615 | 2 |
B6_media |
Peripheral blood monuclear cells |
media alone for 24hr |
GSM1068616 | 2 |
B7_IL-10 |
Peripheral blood monuclear cells |
IL-10 (10ng/ml) for 24hr |
GSM1068617 | 3 |
C6_media |
Peripheral blood monuclear cells |
media alone for 24hr |
GSM1068618 | 3 |
C7_IL-10 |
Peripheral blood monuclear cells |
IL-10 (10ng/ml) for 24hr |
GSM1068619 | 4 |
D6_media |
Peripheral blood monuclear cells |
media alone for 24hr |
GSM1068620 | 4 |
D7_IL-10 |
Peripheral blood monuclear cells |
IL-10 (10ng/ml) for 24hr |
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