Sample Set Annotation Tool: Subpopulations of CD163 positive macrophages are classically activated in psoriasis-GSE18686

Study Design
Assay
Sample Info
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Purpose

To determine the transcriptional profile associated with macrophage polarization to M1 or M2, we cultured macrophages derived from monocytes for 7 days and treated them with IFNg, IL-4, TNF, LPS, and LPS+IFNg (n=7). Psoriasis lesional and non-lesional skin biopsies were included (n=5 pairs).

Hypothesis

Experimental Design

Comparison of the transcriptome of psoriasis with cytokine-treated macrophages.

Experimental Variables

Controls

Methods

Additional Information

Fuentes-Duculan J, Suárez-Fariñas M, Zaba LC, Nograles KE et al. A subpopulation of CD163-positive macrophages is classically activated in psoriasis. J Invest Dermatol 2010 Oct;130(10):2412-22. PMID: 20555352

Expected Samples:

58 Samples Loaded: 58

Species

Human (Homo sapiens)

Sample Source

Monocyte-derived macrophages (MDMs) , Macrophages

Disease

Psoriasis

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Sample ID	!Sample_title	cell type	treatment group
GSM464201	Macrophage culture, ID 1, Control	cultured macrophages	control
GSM464202	Macrophage culture, ID 1, IL17	cultured macrophages	IL17
GSM464203	Macrophage culture, ID 1, IFNg	cultured macrophages	IFNg
GSM464204	Macrophage culture, ID 1, IL4	cultured macrophages	IL4
GSM464205	Macrophage culture, ID 1, TNFa	cultured macrophages	TNFa

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Sample ID	!Sample Title	Cell type	Tissue	Treatment group	Disease status	Batch	ID	Line
GSM464201	Macrophage culture, ID 1, Control	cultured macrophages		control		14	1	A
GSM464202	Macrophage culture, ID 1, IL17	cultured macrophages		IL17		14	1	B
GSM464203	Macrophage culture, ID 1, IFNg	cultured macrophages		IFNg		14	1	C
GSM464204	Macrophage culture, ID 1, IL4	cultured macrophages		IL4		14	1	D
GSM464205	Macrophage culture, ID 1, TNFa	cultured macrophages		TNFa		14	1	E
GSM464206	Macrophage culture, ID 1, LPS	cultured macrophages		LPS		14	1	F
GSM464207	Macrophage culture, ID 1, LPSandIFNg	cultured macrophages		LPS and IFNg		14	1	G
GSM464208	Macrophage culture, ID 2, Control	cultured macrophages		control		14	2	H
GSM464209	Macrophage culture, ID 2, IL17	cultured macrophages		IL17		14	2	I
GSM464210	Macrophage culture, ID 2, IFNg	cultured macrophages		IFNg		14	2	J
GSM464211	Macrophage culture, ID 2, IL4	cultured macrophages		IL4		14	2	K
GSM464212	Macrophage culture, ID 2, TNFa	cultured macrophages		TNFa		14	2	L
GSM464213	Macrophage culture, ID 2, LPS	cultured macrophages		LPS		16	2	A
GSM464214	Macrophage culture, ID 2, LPSandIFNg	cultured macrophages		LPS and IFNg		16	2	B
GSM464215	Macrophage culture, ID 3, Control	cultured macrophages		control		16	3	C
GSM464216	Macrophage culture, ID 3, IL17	cultured macrophages		IL17		16	3	D
GSM464217	Macrophage culture, ID 3, IFNg	cultured macrophages		IFNg		16	3	E
GSM464218	Macrophage culture, ID 3, IL4	cultured macrophages		IL4		16	3	F
GSM464219	Macrophage culture, ID 3, TNFa	cultured macrophages		TNFa		16	3	G
GSM464220	Macrophage culture, ID 3, LPS	cultured macrophages		LPS		16	3	H
GSM464221	Macrophage culture, ID 3, LPSandIFNg	cultured macrophages		LPS and IFNg		16	3	I
GSM464222	Macrophage culture, ID 4, Control	cultured macrophages		control		16	4	J
GSM464223	Macrophage culture, ID 4, IL17	cultured macrophages		IL17		16	4	K
GSM464224	Macrophage culture, ID 4, IFNg	cultured macrophages		IFNg		16	4	L
GSM464225	Macrophage culture, ID 4, IL4	cultured macrophages		IL4		69	4	A
GSM464226	Macrophage culture, ID 4, TNFa	cultured macrophages		TNFa		69	4	B
GSM464227	Macrophage culture, ID 4, LPS	cultured macrophages		LPS		69	4	C
GSM464228	Macrophage culture, ID 4, LPSandIFNg	cultured macrophages		LPS and IFNg		69	4	D
GSM464229	Macrophage culture, ID 4, Control (rep)	cultured macrophages		control		69	4	E
GSM464230	Macrophage culture, ID 4, IL17 (rep)	cultured macrophages		IL17		69	4	F
GSM464231	Macrophage culture, ID 4, IFNg (rep)	cultured macrophages		IFNg		69	4	G
GSM464232	Macrophage culture, ID 4, IL4 (rep)	cultured macrophages		IL4		69	4	H
GSM464233	Macrophage culture, ID 4, TNFa (rep)	cultured macrophages		TNFa		69	4	I
GSM464234	Macrophage culture, ID 4, LPS (rep)	cultured macrophages		LPS		69	4	J
GSM464235	Macrophage culture, ID 4, LPSandIFNg (rep)	cultured macrophages		LPS and IFNg		69	4	K
GSM464236	Macrophage culture, ID 5, Control	cultured macrophages		control		71	5	A
GSM464237	Macrophage culture, ID 5, IL17	cultured macrophages		IL17		71	5	B
GSM464238	Macrophage culture, ID 5, IFNg	cultured macrophages		IFNg		71	5	C
GSM464239	Macrophage culture, ID 5, IL4	cultured macrophages		IL4		71	5	D
GSM464240	Macrophage culture, ID 5, TNFa	cultured macrophages		TNFa		71	5	E
GSM464241	Macrophage culture, ID 5, LPS	cultured macrophages		LPS		71	5	F
GSM464242	Macrophage culture, ID 5, LPSandIFNg	cultured macrophages		LPS and IFNg		71	5	G
GSM464243	Macrophage culture, ID 6, IL17	cultured macrophages		IL17		71	6	I
GSM464244	Macrophage culture, ID 6, IFNg	cultured macrophages		IFNg		71	6	J
GSM464245	Macrophage culture, ID 6, IL4	cultured macrophages		IL4		71	6	K
GSM464246	Macrophage culture, ID 6, TNFa	cultured macrophages		TNFa		71	6	L
GSM464247	Macrophage culture, ID 6, LPS	cultured macrophages		LPS		71	6	A
GSM464248	Macrophage culture, ID 6, LPSandIFNg	cultured macrophages		LPS and IFNg		35	6	B
GSM464249	Psoriasis skin, ID P 001, NL		skin	Non-Lesional	non-lesional psoriasis	35	P 001	C
GSM464250	Psoriasis skin, ID P 002, LS		skin	Lesional	lesional psoriasis	35	P 001	D
GSM464251	Psoriasis skin, ID P 003, NL		skin	Non-Lesional	non-lesional psoriasis	35	P 002	E
GSM464252	Psoriasis skin, ID P 004, LS		skin	Lesional	lesional psoriasis	35	P 002	F
GSM464253	Psoriasis skin, ID P 005, NL		skin	Non-Lesional	non-lesional psoriasis	35	P 003	G
GSM464254	Psoriasis skin, ID P 006, LS		skin	Lesional	lesional psoriasis	35	P 003	H
GSM464255	Psoriasis skin, ID P 007, NL		skin	Non-Lesional	non-lesional psoriasis	35	P 004	I
GSM464256	Psoriasis skin, ID P 008, LS		skin	Lesional	lesional psoriasis	35	P 004	J
GSM464257	Psoriasis skin, ID P 009, NL		skin	Non-Lesional	non-lesional psoriasis	35	P 005	K
GSM464258	Psoriasis skin, ID P 010, LS		skin	Lesional	lesional psoriasis	35	P 005	L

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