Expression data from human macrophages -GSE16385
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Human CD14 positive monocytes were purified from healthy volunteers’ blood and cultured in vitro for 4, 12, 24, 72 hours. While culturing, macrophages were activated alternatively with interleukin-4 (IL-4 100 ng/ml) or classically with interferon-gamma (IFNg 100 ng/ml)+tumor necrosis factor (TNF 50 ng/ml) or left without activation. Simultaneously, macrophages were also treated with vehicle (DMSO:ethanol) or 1mM synthetic PPARg agonist, Rosiglitazone. We used Affymetrix microarrays (U133Plus 2.0) to analyze activation and PPARg-induced gene expression changes.
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Sample ID | !Sample_title | donor | treatment | time | tissue |
---|---|---|---|---|---|
GSM411183 | 1 Monocyte | 1 | None | 0h | monocyte |
GSM411184 | 1 Control macrophage, 4h | 1 | Control | 4h | macrophage |
GSM411185 | 1 Control macrophage+Rosigl., 4h | 1 | Rosiglitazone | 4h | macrophage |
GSM411186 | 1 IL-4 macrophage, 4h | 1 | Control | 4h | macrophage |
GSM411187 | 1 IL-4 macrophage+Rosigl., 4h | 1 | Rosiglitazone | 4h | macrophage |
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Sample ID | !Sample Title | Donor | Treatment | Time | Tissue | Activation |
---|---|---|---|---|---|---|
GSM411183 | 1 Monocyte |
1 |
None |
0h |
monocyte |
None |
GSM411184 | 1 Control macrophage, 4h |
1 |
Control |
4h |
macrophage |
Control |
GSM411185 | 1 Control macrophage+Rosigl., 4h |
1 |
Rosiglitazone |
4h |
macrophage |
Control |
GSM411186 | 1 IL-4 macrophage, 4h |
1 |
Control |
4h |
macrophage |
IL-4 |
GSM411187 | 1 IL-4 macrophage+Rosigl., 4h |
1 |
Rosiglitazone |
4h |
macrophage |
IL-4 |
GSM411188 | 1 IFNg+TNF macrophage, 4h |
1 |
Control |
4h |
macrophage |
IFNg+TNF |
GSM411189 | 1 IFNg+TNF macrophage+Rosigl., 4h |
1 |
Rosiglitazone |
4h |
macrophage |
IFNg+TNF |
GSM411190 | 1 Control macrophage, 12h |
1 |
Control |
12h |
macrophage |
Control |
GSM411191 | 1 Control macrophage+Rosigl., 12h |
1 |
Rosiglitazone |
12h |
macrophage |
Control |
GSM411192 | 1 IL-4 macrophage, 12h |
1 |
Control |
12h |
macrophage |
IL-4 |
GSM411193 | 1 IL-4 macrophage+Rosigl., 12h |
1 |
Rosiglitazone |
12h |
macrophage |
IL-4 |
GSM411194 | 1 IFNg+TNF macrophage,12h |
1 |
Control |
12h |
macrophage |
IFNg+TNF |
GSM411195 | 1 IFNg+TNF macrophage+Rosigl., 12h |
1 |
Rosiglitazone |
12h |
macrophage |
IFNg+TNF |
GSM411196 | 2 Monocyte |
2 |
None |
0h |
monocyte |
None |
GSM411197 | 2 Control macrophage, 12h |
2 |
Control |
12h |
macrophage |
Control |
GSM411198 | 2 Control macrophage+Rosigl., 12h |
2 |
Rosiglitazone |
12h |
macrophage |
Control |
GSM411199 | 2 IL-4 macrophage, 12h |
2 |
Control |
12h |
macrophage |
IL-4 |
GSM411200 | 2 IL-4 macrophage+Rosigl., 12h |
2 |
Rosiglitazone |
12h |
macrophage |
IL-4 |
GSM411201 | 2 IFNg+TNF macrophage, 12h |
2 |
Control |
12h |
macrophage |
IFNg+TNF |
GSM411202 | 2 IFNg+TNF macrophage+Rosigl., 12h |
2 |
Rosiglitazone |
12h |
macrophage |
IFNg+TNF |
GSM411203 | 2 Control macrophage, 24h |
2 |
Control |
24h |
macrophage |
Control |
GSM411204 | 2 Control macrophage+Rosigl., 24h |
2 |
Rosiglitazone |
24h |
macrophage |
Control |
GSM411205 | 2 IL-4 macrophage, 24h |
2 |
Control |
24h |
macrophage |
IL-4 |
GSM411206 | 2 IL-4 macrophage+Rosigl., 24h |
2 |
Rosiglitazone |
24h |
macrophage |
IL-4 |
GSM411207 | 2 IFNg+TNF macrophage, 24h |
2 |
Control |
24h |
macrophage |
IFNg+TNF |
GSM411208 | 2 IFNg+TNF macrophage+Rosigl., 24h |
2 |
Rosiglitazone |
24h |
macrophage |
IFNg+TNF |
GSM411209 | 3 Control macrophage, 12h |
3 |
Control |
12h |
macrophage |
Control |
GSM411210 | 3 Control macrophage+Rosigl., 12h |
3 |
Rosiglitazone |
12h |
macrophage |
Control |
GSM411211 | 3 IL-4 macrophage, 12h |
3 |
Control |
12h |
macrophage |
IL-4 |
GSM411212 | 3 IL-4 macrophage+Rosigl., 12h |
3 |
Rosiglitazone |
12h |
macrophage |
IL-4 |
GSM411213 | 3 IFNg+TNF macrophage, 12h |
3 |
Control |
12h |
macrophage |
IFNg+TNF |
GSM411214 | 3 IFNg+TNF macrophage+Rosigl., 12h |
3 |
Rosiglitazone |
12h |
macrophage |
IFNg+TNF |
GSM411215 | 3 Control macrophage, 72h |
3 |
Control |
72h |
macrophage |
Control |
GSM411216 | 3 Control macrophage+Rosigl., 72h |
3 |
Rosiglitazone |
72h |
macrophage |
Control |
GSM411217 | 3 IL-4 macrophage, 72h |
3 |
Control |
72h |
macrophage |
IL-4 |
GSM411218 | 3 IL-4 macrophage+Rosigl., 72h |
3 |
Rosiglitazone |
72h |
macrophage |
IL-4 |
GSM411219 | 3 IFNg+TNF macrophage, 72h |
3 |
Control |
72h |
macrophage |
IFNg+TNF |
GSM411220 | 3 IFNg+TNF macrophage+Rosigl., 72h |
3 |
Rosiglitazone |
72h |
macrophage |
IFNg+TNF |
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